First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2020.105335 http://hdl.handle.net/11449/201104 |
Resumo: | The protozoan parasite Toxoplasma gondii can infect virtually all warm-blooded animals worldwide but little is known of its infection in the endangered giant anteaters (Myrmecophaga tridactyla). The present study found antibodies to T. gondii in 13 of 23 free-living M. tridactyla from the northwest region of São Paulo state, Brazil, by the Modified Agglutination Test (MAT, cut-off titer 1:25). Unfrozen tissues of seven M. tridactyla were bioassayed in mice and viable T. gondii (strain designated TgMytrBrSP1) isolated from one seropositive giant anteater. To our knowledge, this is a new host record for T. gondii. Genotyping using PCR-RFLP revealed the Brazilian clonal Type BrIII genotype, and a unique non-archetypal genotype was revealed by microsatellite analysis. |
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First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotypeGenotypingGiant anteaterIsolationMicrosatellitesPCR-RFLPToxoplasmosisThe protozoan parasite Toxoplasma gondii can infect virtually all warm-blooded animals worldwide but little is known of its infection in the endangered giant anteaters (Myrmecophaga tridactyla). The present study found antibodies to T. gondii in 13 of 23 free-living M. tridactyla from the northwest region of São Paulo state, Brazil, by the Modified Agglutination Test (MAT, cut-off titer 1:25). Unfrozen tissues of seven M. tridactyla were bioassayed in mice and viable T. gondii (strain designated TgMytrBrSP1) isolated from one seropositive giant anteater. To our knowledge, this is a new host record for T. gondii. Genotyping using PCR-RFLP revealed the Brazilian clonal Type BrIII genotype, and a unique non-archetypal genotype was revealed by microsatellite analysis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Doenças Parasitárias Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS) Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87Universidade Estadual Paulista “Júlio de Mesquita” Filho (UNESP) Instituto de Biociências Letras e Ciências Exatas (IBILCE), Campus de São José do Rio PretoFAMERP Toxoplasma Research Group Departamento de Biologia Molecular Faculdade de Medicina de São José do Rio Preto (FAMERP)Centro Universitário de Rio Preto UNIRPAnimal Parasitic Diseases Laboratory Beltsville Agricultural Research Center United States Department of AgricultureDepartamento de Epidemiologia e Saúde Coletiva Faculdade de Medicina de São José do Rio Preto (FAMERP)Universidade Estadual Paulista “Júlio de Mesquita” Filho (UNESP) Instituto de Biociências Letras e Ciências Exatas (IBILCE), Campus de São José do Rio PretoCAPES: 001FAPESP: 14/25872-3FAPESP: 2014/05302-8Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Faculdade de Medicina de São José do Rio Preto (FAMERP)UNIRPUnited States Department of AgriculturePena, Hilda Fátima JesusFerrari, Vinícius Matheus [UNESP]Aires, Luiz Paulo NogueiraSoares, Herbert SousaOliveira, SolangeAlves, Bruna FariasGennari, Solange MariaDubey, Jitender P.de Mattos, Luiz Carlosde Mattos, Cinara Cássia BrandãoCastiglioni, Lilian [UNESP]2020-12-12T02:24:13Z2020-12-12T02:24:13Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2020.105335Acta Tropica, v. 204.1873-62540001-706Xhttp://hdl.handle.net/11449/20110410.1016/j.actatropica.2020.1053352-s2.0-85079234901Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2021-10-23T16:01:11Zoai:repositorio.unesp.br:11449/201104Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:01:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
title |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
spellingShingle |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype Pena, Hilda Fátima Jesus Genotyping Giant anteater Isolation Microsatellites PCR-RFLP Toxoplasmosis |
title_short |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
title_full |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
title_fullStr |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
title_full_unstemmed |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
title_sort |
First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype |
author |
Pena, Hilda Fátima Jesus |
author_facet |
Pena, Hilda Fátima Jesus Ferrari, Vinícius Matheus [UNESP] Aires, Luiz Paulo Nogueira Soares, Herbert Sousa Oliveira, Solange Alves, Bruna Farias Gennari, Solange Maria Dubey, Jitender P. de Mattos, Luiz Carlos de Mattos, Cinara Cássia Brandão Castiglioni, Lilian [UNESP] |
author_role |
author |
author2 |
Ferrari, Vinícius Matheus [UNESP] Aires, Luiz Paulo Nogueira Soares, Herbert Sousa Oliveira, Solange Alves, Bruna Farias Gennari, Solange Maria Dubey, Jitender P. de Mattos, Luiz Carlos de Mattos, Cinara Cássia Brandão Castiglioni, Lilian [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Faculdade de Medicina de São José do Rio Preto (FAMERP) UNIRP United States Department of Agriculture |
dc.contributor.author.fl_str_mv |
Pena, Hilda Fátima Jesus Ferrari, Vinícius Matheus [UNESP] Aires, Luiz Paulo Nogueira Soares, Herbert Sousa Oliveira, Solange Alves, Bruna Farias Gennari, Solange Maria Dubey, Jitender P. de Mattos, Luiz Carlos de Mattos, Cinara Cássia Brandão Castiglioni, Lilian [UNESP] |
dc.subject.por.fl_str_mv |
Genotyping Giant anteater Isolation Microsatellites PCR-RFLP Toxoplasmosis |
topic |
Genotyping Giant anteater Isolation Microsatellites PCR-RFLP Toxoplasmosis |
description |
The protozoan parasite Toxoplasma gondii can infect virtually all warm-blooded animals worldwide but little is known of its infection in the endangered giant anteaters (Myrmecophaga tridactyla). The present study found antibodies to T. gondii in 13 of 23 free-living M. tridactyla from the northwest region of São Paulo state, Brazil, by the Modified Agglutination Test (MAT, cut-off titer 1:25). Unfrozen tissues of seven M. tridactyla were bioassayed in mice and viable T. gondii (strain designated TgMytrBrSP1) isolated from one seropositive giant anteater. To our knowledge, this is a new host record for T. gondii. Genotyping using PCR-RFLP revealed the Brazilian clonal Type BrIII genotype, and a unique non-archetypal genotype was revealed by microsatellite analysis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:24:13Z 2020-12-12T02:24:13Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2020.105335 Acta Tropica, v. 204. 1873-6254 0001-706X http://hdl.handle.net/11449/201104 10.1016/j.actatropica.2020.105335 2-s2.0-85079234901 |
url |
http://dx.doi.org/10.1016/j.actatropica.2020.105335 http://hdl.handle.net/11449/201104 |
identifier_str_mv |
Acta Tropica, v. 204. 1873-6254 0001-706X 10.1016/j.actatropica.2020.105335 2-s2.0-85079234901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965550970929152 |