New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes

Detalhes bibliográficos
Autor(a) principal: Alves, Leticia Rodrigues [UNESP]
Data de Publicação: 2019
Outros Autores: Rodrigues dos Reis, André [UNESP], Prado, Emilaine Rocha [UNESP], Lavres, José, Pompeu, Georgia Bertoni, Azevedo, Ricardo Antunes, Gratão, Priscila Lupino [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ecoenv.2019.109747
http://hdl.handle.net/11449/199505
Resumo: Cadmium (Cd) contamination has generated an environmental problem worldwide, leading to harmful effects on human health and damages to plant metabolism. Selenium (Se) is non essential for plants, however it can improve plant growth and reduce the adverse effects of abiotic stress. In addition, ethylene may interplay the positive effects of Se in plants. In order to investigate the role of ethylene in Se-modulation of antioxidant defence system in response to Cd-stress, we tested the hormonal mutant Epinastic (epi) with a subset of constitutive activation of the ethylene response and Micro-Tom (MT) plants. For this purpose, Se mineral uptake, Cd and Se concentrations, pigments, malondialdeyde (MDA) and hydrogen peroxide (H2O2) contents, ethylene production, glutathione (GSH) compound, and superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) activities were analysed in MT and epi plants submitted to 0.5 mM CdCl2 and 1 μM of selenate or selenite. MT plants treated with both Se forms increased growth in the presence or not of 0.5 mM CdCl2, but not change epi growth. Both Se forms reduced Cd uptake in MT plants and cause reverse effect in epi plants. P, Mg, S, K and Zn uptake increased in epi plants with Se application, irrespective to Cd exposure. Chlorophylls and carotenoids contents decreased in both genotypes under Cd exposure, in contrast to what was observed in epi leaves in the presence of Se. When antioxidant enzymes activities were concerned, Se application increased Mn-SOD, Fe-SOD and APX activities. In the presence of Cd, MT and epi plants exhibited decreased SOD activity and increased CAT, APX and GR activities. MT and epi plants with Se supply exhibited increased APX and GR activities in the presence of Cd. Overall, these results suggest that ethylene may be involved in Se induced-defence responses, that triggers a positive response of the antioxidant system and improve growth under Cd stress. These results showed integrative roles of ethylene and Se in regulating the cell responses to stressful-conditions and, the cross-tolerance to stress could be used to manipulate ethylene regulated gene expression to induce heavy metal tolerance.
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spelling New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymesAntioxidant systemEthyleneMutantsReactive oxygen speciesTomatoCadmium (Cd) contamination has generated an environmental problem worldwide, leading to harmful effects on human health and damages to plant metabolism. Selenium (Se) is non essential for plants, however it can improve plant growth and reduce the adverse effects of abiotic stress. In addition, ethylene may interplay the positive effects of Se in plants. In order to investigate the role of ethylene in Se-modulation of antioxidant defence system in response to Cd-stress, we tested the hormonal mutant Epinastic (epi) with a subset of constitutive activation of the ethylene response and Micro-Tom (MT) plants. For this purpose, Se mineral uptake, Cd and Se concentrations, pigments, malondialdeyde (MDA) and hydrogen peroxide (H2O2) contents, ethylene production, glutathione (GSH) compound, and superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) activities were analysed in MT and epi plants submitted to 0.5 mM CdCl2 and 1 μM of selenate or selenite. MT plants treated with both Se forms increased growth in the presence or not of 0.5 mM CdCl2, but not change epi growth. Both Se forms reduced Cd uptake in MT plants and cause reverse effect in epi plants. P, Mg, S, K and Zn uptake increased in epi plants with Se application, irrespective to Cd exposure. Chlorophylls and carotenoids contents decreased in both genotypes under Cd exposure, in contrast to what was observed in epi leaves in the presence of Se. When antioxidant enzymes activities were concerned, Se application increased Mn-SOD, Fe-SOD and APX activities. In the presence of Cd, MT and epi plants exhibited decreased SOD activity and increased CAT, APX and GR activities. MT and epi plants with Se supply exhibited increased APX and GR activities in the presence of Cd. Overall, these results suggest that ethylene may be involved in Se induced-defence responses, that triggers a positive response of the antioxidant system and improve growth under Cd stress. These results showed integrative roles of ethylene and Se in regulating the cell responses to stressful-conditions and, the cross-tolerance to stress could be used to manipulate ethylene regulated gene expression to induce heavy metal tolerance.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias. Departamento de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências e Engenharia Laboratório de BiologiaUniversidade de São Paulo (USP) Centro de Energia Nuclear na Agricultura Laboratório de Nutrição Mineral de PlantasUSP - Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz-ESALQ Depto. de Ciencia do SoloUniversidade de São Paulo (USP) Escola Superior de Agricultura Luiz de Queiroz (ESALQ)Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias. Departamento de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências e Engenharia Laboratório de BiologiaCAPES: 001FAPESP: 2017/04787-6CNPq: 303749/2016-4CNPq: 309380/2017-0CNPq: 314380/2018-3CNPq: 448783/2014-2Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Alves, Leticia Rodrigues [UNESP]Rodrigues dos Reis, André [UNESP]Prado, Emilaine Rocha [UNESP]Lavres, JoséPompeu, Georgia BertoniAzevedo, Ricardo AntunesGratão, Priscila Lupino [UNESP]2020-12-12T01:41:46Z2020-12-12T01:41:46Z2019-12-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2019.109747Ecotoxicology and Environmental Safety, v. 186.1090-24140147-6513http://hdl.handle.net/11449/19950510.1016/j.ecoenv.2019.1097472-s2.0-85073433180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2024-06-06T13:04:11Zoai:repositorio.unesp.br:11449/199505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:28:36.970495Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
title New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
spellingShingle New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
Alves, Leticia Rodrigues [UNESP]
Antioxidant system
Ethylene
Mutants
Reactive oxygen species
Tomato
title_short New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
title_full New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
title_fullStr New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
title_full_unstemmed New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
title_sort New insights into cadmium stressful-conditions: Role of ethylene on selenium-mediated antioxidant enzymes
author Alves, Leticia Rodrigues [UNESP]
author_facet Alves, Leticia Rodrigues [UNESP]
Rodrigues dos Reis, André [UNESP]
Prado, Emilaine Rocha [UNESP]
Lavres, José
Pompeu, Georgia Bertoni
Azevedo, Ricardo Antunes
Gratão, Priscila Lupino [UNESP]
author_role author
author2 Rodrigues dos Reis, André [UNESP]
Prado, Emilaine Rocha [UNESP]
Lavres, José
Pompeu, Georgia Bertoni
Azevedo, Ricardo Antunes
Gratão, Priscila Lupino [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Alves, Leticia Rodrigues [UNESP]
Rodrigues dos Reis, André [UNESP]
Prado, Emilaine Rocha [UNESP]
Lavres, José
Pompeu, Georgia Bertoni
Azevedo, Ricardo Antunes
Gratão, Priscila Lupino [UNESP]
dc.subject.por.fl_str_mv Antioxidant system
Ethylene
Mutants
Reactive oxygen species
Tomato
topic Antioxidant system
Ethylene
Mutants
Reactive oxygen species
Tomato
description Cadmium (Cd) contamination has generated an environmental problem worldwide, leading to harmful effects on human health and damages to plant metabolism. Selenium (Se) is non essential for plants, however it can improve plant growth and reduce the adverse effects of abiotic stress. In addition, ethylene may interplay the positive effects of Se in plants. In order to investigate the role of ethylene in Se-modulation of antioxidant defence system in response to Cd-stress, we tested the hormonal mutant Epinastic (epi) with a subset of constitutive activation of the ethylene response and Micro-Tom (MT) plants. For this purpose, Se mineral uptake, Cd and Se concentrations, pigments, malondialdeyde (MDA) and hydrogen peroxide (H2O2) contents, ethylene production, glutathione (GSH) compound, and superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) activities were analysed in MT and epi plants submitted to 0.5 mM CdCl2 and 1 μM of selenate or selenite. MT plants treated with both Se forms increased growth in the presence or not of 0.5 mM CdCl2, but not change epi growth. Both Se forms reduced Cd uptake in MT plants and cause reverse effect in epi plants. P, Mg, S, K and Zn uptake increased in epi plants with Se application, irrespective to Cd exposure. Chlorophylls and carotenoids contents decreased in both genotypes under Cd exposure, in contrast to what was observed in epi leaves in the presence of Se. When antioxidant enzymes activities were concerned, Se application increased Mn-SOD, Fe-SOD and APX activities. In the presence of Cd, MT and epi plants exhibited decreased SOD activity and increased CAT, APX and GR activities. MT and epi plants with Se supply exhibited increased APX and GR activities in the presence of Cd. Overall, these results suggest that ethylene may be involved in Se induced-defence responses, that triggers a positive response of the antioxidant system and improve growth under Cd stress. These results showed integrative roles of ethylene and Se in regulating the cell responses to stressful-conditions and, the cross-tolerance to stress could be used to manipulate ethylene regulated gene expression to induce heavy metal tolerance.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-30
2020-12-12T01:41:46Z
2020-12-12T01:41:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ecoenv.2019.109747
Ecotoxicology and Environmental Safety, v. 186.
1090-2414
0147-6513
http://hdl.handle.net/11449/199505
10.1016/j.ecoenv.2019.109747
2-s2.0-85073433180
url http://dx.doi.org/10.1016/j.ecoenv.2019.109747
http://hdl.handle.net/11449/199505
identifier_str_mv Ecotoxicology and Environmental Safety, v. 186.
1090-2414
0147-6513
10.1016/j.ecoenv.2019.109747
2-s2.0-85073433180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ecotoxicology and Environmental Safety
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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