Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle

Detalhes bibliográficos
Autor(a) principal: Arnold, Daniel R. [UNESP]
Data de Publicação: 2017
Outros Autores: Gaspar, Roberta C. [UNESP], Rocha, Carlos V. da [UNESP], Sangalli, Juliano R., Bem, Tiago H. C. de, Correa, Carolina A. P. [UNESP], Penteado, Joao C. T. [UNESP], Meirelles, Flavio V., Lopes, Flavia L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1071/RD15132
http://hdl.handle.net/11449/159412
Resumo: Abnormal placental development is frequent in nuclear transfer (NT) pregnancies and is likely to be associated with altered epigenetic reprogramming. In the present study, fetal and placental measurements were taken on Day 60 of gestation in cows with pregnancies produced by AI, IVF and NT. Placentas were collected and subjected to histological evaluation, the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2), as well as chromatin immunoprecipitation (ChIP) for histone marks within the promoter of PHLDA2. Fewer binucleated cells were observed in NT cotyledons, followed by IVF and AI cotyledons (P<0.05). Expression of heart and neural crest derivatives expressed 1 (HAND1), placental lactogen (PL), pregnancy-associated glycoprotein 9 (PAG-9) and PHLDA2 was elevated in NT cotyledons compared with AI cotyledons. Expression of PHLDA2 was higher in IVF than AI samples (P<0.05). ChIP revealed an increase in the permissive mark dimethylation of lysine 4 on histone H3 (H3K4me2), surprisingly associated with the silent allele of PHLDA2, and a decrease in the inhibitory mark H3K9me2 in NT samples. Thus, genes critical for placental development were altered in NT placentas, including an imprinted gene. Allele-specific changes in the permissive histone mark in the PHLDA2 promoter indicate misregulation of imprinting in clones. Abnormal trophoblast differentiation could have resulted in lower numbers of binucleated cells following NT. These results suggest that the altered expression of imprinted genes associated with NT are also caused by changes in histone modifications.
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spelling Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattleassisted reproductive technologiesbovinecotyledonsH3K4me2H3K9me2mRNAAbnormal placental development is frequent in nuclear transfer (NT) pregnancies and is likely to be associated with altered epigenetic reprogramming. In the present study, fetal and placental measurements were taken on Day 60 of gestation in cows with pregnancies produced by AI, IVF and NT. Placentas were collected and subjected to histological evaluation, the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2), as well as chromatin immunoprecipitation (ChIP) for histone marks within the promoter of PHLDA2. Fewer binucleated cells were observed in NT cotyledons, followed by IVF and AI cotyledons (P<0.05). Expression of heart and neural crest derivatives expressed 1 (HAND1), placental lactogen (PL), pregnancy-associated glycoprotein 9 (PAG-9) and PHLDA2 was elevated in NT cotyledons compared with AI cotyledons. Expression of PHLDA2 was higher in IVF than AI samples (P<0.05). ChIP revealed an increase in the permissive mark dimethylation of lysine 4 on histone H3 (H3K4me2), surprisingly associated with the silent allele of PHLDA2, and a decrease in the inhibitory mark H3K9me2 in NT samples. Thus, genes critical for placental development were altered in NT placentas, including an imprinted gene. Allele-specific changes in the permissive histone mark in the PHLDA2 promoter indicate misregulation of imprinting in clones. Abnormal trophoblast differentiation could have resulted in lower numbers of binucleated cells following NT. These results suggest that the altered expression of imprinted genes associated with NT are also caused by changes in histone modifications.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Sao Paulo State Univ, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, BrazilUniv Sao Paulo, Fac Zootecnia & Engn Alimentos, BR-13635900 Sao Paulo, BrazilUniv Estadual Paulista, Sao Paulo State Univ, Fac Med Vet, BR-16050680 Sao Paulo, BrazilIn Vitro Brasil SA, BR-13800970 Sao Paulo, BrazilUniv Estadual Paulista, Sao Paulo State Univ, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, BrazilUniv Estadual Paulista, Sao Paulo State Univ, Fac Med Vet, BR-16050680 Sao Paulo, BrazilFAPESP: 2009/50381-5Csiro PublishingUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)In Vitro Brasil SAArnold, Daniel R. [UNESP]Gaspar, Roberta C. [UNESP]Rocha, Carlos V. da [UNESP]Sangalli, Juliano R.Bem, Tiago H. C. deCorrea, Carolina A. P. [UNESP]Penteado, Joao C. T. [UNESP]Meirelles, Flavio V.Lopes, Flavia L. [UNESP]2018-11-26T15:43:40Z2018-11-26T15:43:40Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article458-467http://dx.doi.org/10.1071/RD15132Reproduction Fertility And Development. Clayton: Csiro Publishing, v. 29, n. 3, p. 458-467, 2017.1031-3613http://hdl.handle.net/11449/15941210.1071/RD15132WOS:000395555500003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction Fertility And Development0,681info:eu-repo/semantics/openAccess2021-10-23T15:48:43Zoai:repositorio.unesp.br:11449/159412Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:26:29.506251Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
title Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
spellingShingle Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
Arnold, Daniel R. [UNESP]
assisted reproductive technologies
bovine
cotyledons
H3K4me2
H3K9me2
mRNA
title_short Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
title_full Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
title_fullStr Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
title_full_unstemmed Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
title_sort Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle
author Arnold, Daniel R. [UNESP]
author_facet Arnold, Daniel R. [UNESP]
Gaspar, Roberta C. [UNESP]
Rocha, Carlos V. da [UNESP]
Sangalli, Juliano R.
Bem, Tiago H. C. de
Correa, Carolina A. P. [UNESP]
Penteado, Joao C. T. [UNESP]
Meirelles, Flavio V.
Lopes, Flavia L. [UNESP]
author_role author
author2 Gaspar, Roberta C. [UNESP]
Rocha, Carlos V. da [UNESP]
Sangalli, Juliano R.
Bem, Tiago H. C. de
Correa, Carolina A. P. [UNESP]
Penteado, Joao C. T. [UNESP]
Meirelles, Flavio V.
Lopes, Flavia L. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
In Vitro Brasil SA
dc.contributor.author.fl_str_mv Arnold, Daniel R. [UNESP]
Gaspar, Roberta C. [UNESP]
Rocha, Carlos V. da [UNESP]
Sangalli, Juliano R.
Bem, Tiago H. C. de
Correa, Carolina A. P. [UNESP]
Penteado, Joao C. T. [UNESP]
Meirelles, Flavio V.
Lopes, Flavia L. [UNESP]
dc.subject.por.fl_str_mv assisted reproductive technologies
bovine
cotyledons
H3K4me2
H3K9me2
mRNA
topic assisted reproductive technologies
bovine
cotyledons
H3K4me2
H3K9me2
mRNA
description Abnormal placental development is frequent in nuclear transfer (NT) pregnancies and is likely to be associated with altered epigenetic reprogramming. In the present study, fetal and placental measurements were taken on Day 60 of gestation in cows with pregnancies produced by AI, IVF and NT. Placentas were collected and subjected to histological evaluation, the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2), as well as chromatin immunoprecipitation (ChIP) for histone marks within the promoter of PHLDA2. Fewer binucleated cells were observed in NT cotyledons, followed by IVF and AI cotyledons (P<0.05). Expression of heart and neural crest derivatives expressed 1 (HAND1), placental lactogen (PL), pregnancy-associated glycoprotein 9 (PAG-9) and PHLDA2 was elevated in NT cotyledons compared with AI cotyledons. Expression of PHLDA2 was higher in IVF than AI samples (P<0.05). ChIP revealed an increase in the permissive mark dimethylation of lysine 4 on histone H3 (H3K4me2), surprisingly associated with the silent allele of PHLDA2, and a decrease in the inhibitory mark H3K9me2 in NT samples. Thus, genes critical for placental development were altered in NT placentas, including an imprinted gene. Allele-specific changes in the permissive histone mark in the PHLDA2 promoter indicate misregulation of imprinting in clones. Abnormal trophoblast differentiation could have resulted in lower numbers of binucleated cells following NT. These results suggest that the altered expression of imprinted genes associated with NT are also caused by changes in histone modifications.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-11-26T15:43:40Z
2018-11-26T15:43:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1071/RD15132
Reproduction Fertility And Development. Clayton: Csiro Publishing, v. 29, n. 3, p. 458-467, 2017.
1031-3613
http://hdl.handle.net/11449/159412
10.1071/RD15132
WOS:000395555500003
url http://dx.doi.org/10.1071/RD15132
http://hdl.handle.net/11449/159412
identifier_str_mv Reproduction Fertility And Development. Clayton: Csiro Publishing, v. 29, n. 3, p. 458-467, 2017.
1031-3613
10.1071/RD15132
WOS:000395555500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproduction Fertility And Development
0,681
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 458-467
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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