Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016001300014 http://hdl.handle.net/11449/179738 |
Resumo: | This study aimed to evaluate the proteinogram of the colostrum of ewes submitted to administration of propylene glycol and cobalt associated with vitamin B12 in late pregnancy. Eighteen pregnant Santa Inês ewes 18 months to 5 years old were distributed by probabilistic sampling into three experimental groups, about 30 days before the expected delivery date. In group 1 (G1/n=6), daily oral doses of 30ml propylene glycol PA were administered; Group 2 (G2/n=2) received a daily oral dosage of 1mg cobalt chloride in 1% solution and 2mg of vitamin B12 intramuscularly once a week, and Group 3 (G3/n=6) was the control group. Soon after delivery 30mL of colostrum was harvested from each ewe, which were stored in appropriate containers and sent to the laboratory. After homogenization, we added to each 1000μL of colostrum 75μL solution of rennin, which was kept in a water bath at 37°C for about 20 minutes and centrifuged at 21.000G for 10 minutes in a refrigerated centrifuge. Later, the intermediate fraction, corresponding to colostrum whey, was aliquoted and kept in a -80oC ultrafreezer for subsequent determination of proteins. The determination of the total colostral protein whey was performed using a commercial reagent, observing the linearity test for colostrum. The separation of proteins was performed using the technique of electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). Lactoferrin, IgA, albumin, IgG heavy chain (IgGCP), β-casein, IgG light chain (IgGCL), β-lactoglobulin and α-lactalbumin proteins were identified. There was no influence of the administration of supplements during late pregnancy on the concentration of proteins identified in the colostrum of the ewes. |
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Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestaçãoElectrophoretic profile of the colostrum of ewes supplemented with propylene glycol and cobalt associated with vitamin B12 in late pregnancyColostrumGluconeogenic supplementsPeripartumSDS-PAGESheepWhey proteinsThis study aimed to evaluate the proteinogram of the colostrum of ewes submitted to administration of propylene glycol and cobalt associated with vitamin B12 in late pregnancy. Eighteen pregnant Santa Inês ewes 18 months to 5 years old were distributed by probabilistic sampling into three experimental groups, about 30 days before the expected delivery date. In group 1 (G1/n=6), daily oral doses of 30ml propylene glycol PA were administered; Group 2 (G2/n=2) received a daily oral dosage of 1mg cobalt chloride in 1% solution and 2mg of vitamin B12 intramuscularly once a week, and Group 3 (G3/n=6) was the control group. Soon after delivery 30mL of colostrum was harvested from each ewe, which were stored in appropriate containers and sent to the laboratory. After homogenization, we added to each 1000μL of colostrum 75μL solution of rennin, which was kept in a water bath at 37°C for about 20 minutes and centrifuged at 21.000G for 10 minutes in a refrigerated centrifuge. Later, the intermediate fraction, corresponding to colostrum whey, was aliquoted and kept in a -80oC ultrafreezer for subsequent determination of proteins. The determination of the total colostral protein whey was performed using a commercial reagent, observing the linearity test for colostrum. The separation of proteins was performed using the technique of electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). Lactoferrin, IgA, albumin, IgG heavy chain (IgGCP), β-casein, IgG light chain (IgGCL), β-lactoglobulin and α-lactalbumin proteins were identified. There was no influence of the administration of supplements during late pregnancy on the concentration of proteins identified in the colostrum of the ewes.Universidade Federal Rural de Pernambuco (UFRPE), Av. Dom Manuel de Medeiros s/n, Dois IrmãosClínica de Bovinos UFRPE, Campus Garanhuns, Av. Bom Pastor s/n, Boa Vista, Cx. Postal 152Departamento de Medicina Veterinária UFRPE, Av. Dom Manuel de Medeiros s/n, Dois IrmãosFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Campus de Jaboticabal, Via de acesso Prof. Paulo Donato Castellane s/nFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Campus de Jaboticabal, Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Federal Rural de Pernambuco (UFRPE)UFRPEUniversidade Estadual Paulista (Unesp)Campos, Anne Grace S.S.dos Santos, Rogério A.Afonso, José Augusto B.Soares, Pierre C.Fagliari, José J. [UNESP]Silva, Paulo C. [UNESP]de Mendonça, Carla L.2018-12-11T17:36:33Z2018-12-11T17:36:33Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-100application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016001300014Pesquisa Veterinaria Brasileira, v. 36, p. 95-100.1678-51500100-736Xhttp://hdl.handle.net/11449/17973810.1590/S0100-736X2016001300014S0100-736X20160013000952-s2.0-85044849542S0100-736X2016001300095.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-10-05T06:06:35Zoai:repositorio.unesp.br:11449/179738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-05T06:06:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação Electrophoretic profile of the colostrum of ewes supplemented with propylene glycol and cobalt associated with vitamin B12 in late pregnancy |
title |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
spellingShingle |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação Campos, Anne Grace S.S. Colostrum Gluconeogenic supplements Peripartum SDS-PAGE Sheep Whey proteins |
title_short |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
title_full |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
title_fullStr |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
title_full_unstemmed |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
title_sort |
Perfil eletroforético do colostro de ovelhas suplementadas com propileno glicol e cobalto associado à vitamina B12 no final da gestação |
author |
Campos, Anne Grace S.S. |
author_facet |
Campos, Anne Grace S.S. dos Santos, Rogério A. Afonso, José Augusto B. Soares, Pierre C. Fagliari, José J. [UNESP] Silva, Paulo C. [UNESP] de Mendonça, Carla L. |
author_role |
author |
author2 |
dos Santos, Rogério A. Afonso, José Augusto B. Soares, Pierre C. Fagliari, José J. [UNESP] Silva, Paulo C. [UNESP] de Mendonça, Carla L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Rural de Pernambuco (UFRPE) UFRPE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Campos, Anne Grace S.S. dos Santos, Rogério A. Afonso, José Augusto B. Soares, Pierre C. Fagliari, José J. [UNESP] Silva, Paulo C. [UNESP] de Mendonça, Carla L. |
dc.subject.por.fl_str_mv |
Colostrum Gluconeogenic supplements Peripartum SDS-PAGE Sheep Whey proteins |
topic |
Colostrum Gluconeogenic supplements Peripartum SDS-PAGE Sheep Whey proteins |
description |
This study aimed to evaluate the proteinogram of the colostrum of ewes submitted to administration of propylene glycol and cobalt associated with vitamin B12 in late pregnancy. Eighteen pregnant Santa Inês ewes 18 months to 5 years old were distributed by probabilistic sampling into three experimental groups, about 30 days before the expected delivery date. In group 1 (G1/n=6), daily oral doses of 30ml propylene glycol PA were administered; Group 2 (G2/n=2) received a daily oral dosage of 1mg cobalt chloride in 1% solution and 2mg of vitamin B12 intramuscularly once a week, and Group 3 (G3/n=6) was the control group. Soon after delivery 30mL of colostrum was harvested from each ewe, which were stored in appropriate containers and sent to the laboratory. After homogenization, we added to each 1000μL of colostrum 75μL solution of rennin, which was kept in a water bath at 37°C for about 20 minutes and centrifuged at 21.000G for 10 minutes in a refrigerated centrifuge. Later, the intermediate fraction, corresponding to colostrum whey, was aliquoted and kept in a -80oC ultrafreezer for subsequent determination of proteins. The determination of the total colostral protein whey was performed using a commercial reagent, observing the linearity test for colostrum. The separation of proteins was performed using the technique of electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). Lactoferrin, IgA, albumin, IgG heavy chain (IgGCP), β-casein, IgG light chain (IgGCL), β-lactoglobulin and α-lactalbumin proteins were identified. There was no influence of the administration of supplements during late pregnancy on the concentration of proteins identified in the colostrum of the ewes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:36:33Z 2018-12-11T17:36:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016001300014 Pesquisa Veterinaria Brasileira, v. 36, p. 95-100. 1678-5150 0100-736X http://hdl.handle.net/11449/179738 10.1590/S0100-736X2016001300014 S0100-736X2016001300095 2-s2.0-85044849542 S0100-736X2016001300095.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016001300014 http://hdl.handle.net/11449/179738 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, p. 95-100. 1678-5150 0100-736X 10.1590/S0100-736X2016001300014 S0100-736X2016001300095 2-s2.0-85044849542 S0100-736X2016001300095.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
95-100 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964444215738368 |