Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.syapm.2016.11.002 http://hdl.handle.net/11449/169304 |
Resumo: | Infection by Wolbachia was described previously in eleven species of Anastrepha fruit flies some of which are important pests of fruticulture. One such species is the nominal Anastrepha fraterculus, the South American fruit fly, which actually comprises a complex of cryptic species. The suggestions of using Wolbachia for the control of these pest species, make imperative a more precise characterization of the existing strains of the bacteria. In this study, population samples of the A. fraterculus complex from Brazil, Argentina, Peru, Ecuador, Colombia, Guatemala and Mexico were analyzed for Wolbachia infection. The bacteria were genotyped by the MLST and WSP Typing methodologies. All samples were infected with Wolbachia of supergroup “A”. For each of the five MLST genes, unique as well as already known alleles were detected. Nineteen sequence types for the concatenated sequences of the five MLST genes, and twenty wsp alleles were found in the samples. Host-specific haplotypes, shared strains among distinct hosts, and more than one strain of Wolbachia were found in some population samples. Recombination among the MLST genes and intragenic recombination between wsp haplotypes was rare. Phylogenetic analysis showed a great similarity among the Wolbachia strains in the A. fraterculus complex. However, some strains of Wolbachia are found throughout the Neotropical Region and there are specific strains in determined geographical areas. |
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Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical RegionBacteriaFruit fliesMLSTPhylogenyRecombinationWSP TypingInfection by Wolbachia was described previously in eleven species of Anastrepha fruit flies some of which are important pests of fruticulture. One such species is the nominal Anastrepha fraterculus, the South American fruit fly, which actually comprises a complex of cryptic species. The suggestions of using Wolbachia for the control of these pest species, make imperative a more precise characterization of the existing strains of the bacteria. In this study, population samples of the A. fraterculus complex from Brazil, Argentina, Peru, Ecuador, Colombia, Guatemala and Mexico were analyzed for Wolbachia infection. The bacteria were genotyped by the MLST and WSP Typing methodologies. All samples were infected with Wolbachia of supergroup “A”. For each of the five MLST genes, unique as well as already known alleles were detected. Nineteen sequence types for the concatenated sequences of the five MLST genes, and twenty wsp alleles were found in the samples. Host-specific haplotypes, shared strains among distinct hosts, and more than one strain of Wolbachia were found in some population samples. Recombination among the MLST genes and intragenic recombination between wsp haplotypes was rare. Phylogenetic analysis showed a great similarity among the Wolbachia strains in the A. fraterculus complex. However, some strains of Wolbachia are found throughout the Neotropical Region and there are specific strains in determined geographical areas.Departamento de Genética e Biologia Evolutiva Instituto de Biociências Universidade de São PauloRed de Interacciones Multitróficas Instituto de Ecología A.C.Departamento de Genética Instituto de Biociências UNESPDepartamento de Genética Instituto de Biociências UNESPUniversidade de São Paulo (USP)Instituto de Ecología A.C.Universidade Estadual Paulista (Unesp)Prezotto, Leandro F.Perondini, André L.P.Hernández-Ortiz, VicenteMarino, Celso L. [UNESP]Selivon, Denise2018-12-11T16:45:17Z2018-12-11T16:45:17Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article59-67application/pdfhttp://dx.doi.org/10.1016/j.syapm.2016.11.002Systematic and Applied Microbiology, v. 40, n. 1, p. 59-67, 2017.1618-09840723-2020http://hdl.handle.net/11449/16930410.1016/j.syapm.2016.11.0022-s2.0-850075084922-s2.0-85007508492.pdf01653487382083190000-0003-4524-954XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSystematic and Applied Microbiology1,367info:eu-repo/semantics/openAccess2023-10-09T06:08:18Zoai:repositorio.unesp.br:11449/169304Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:28:52.263829Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
title |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
spellingShingle |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region Prezotto, Leandro F. Bacteria Fruit flies MLST Phylogeny Recombination WSP Typing |
title_short |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
title_full |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
title_fullStr |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
title_full_unstemmed |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
title_sort |
Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region |
author |
Prezotto, Leandro F. |
author_facet |
Prezotto, Leandro F. Perondini, André L.P. Hernández-Ortiz, Vicente Marino, Celso L. [UNESP] Selivon, Denise |
author_role |
author |
author2 |
Perondini, André L.P. Hernández-Ortiz, Vicente Marino, Celso L. [UNESP] Selivon, Denise |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Instituto de Ecología A.C. Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Prezotto, Leandro F. Perondini, André L.P. Hernández-Ortiz, Vicente Marino, Celso L. [UNESP] Selivon, Denise |
dc.subject.por.fl_str_mv |
Bacteria Fruit flies MLST Phylogeny Recombination WSP Typing |
topic |
Bacteria Fruit flies MLST Phylogeny Recombination WSP Typing |
description |
Infection by Wolbachia was described previously in eleven species of Anastrepha fruit flies some of which are important pests of fruticulture. One such species is the nominal Anastrepha fraterculus, the South American fruit fly, which actually comprises a complex of cryptic species. The suggestions of using Wolbachia for the control of these pest species, make imperative a more precise characterization of the existing strains of the bacteria. In this study, population samples of the A. fraterculus complex from Brazil, Argentina, Peru, Ecuador, Colombia, Guatemala and Mexico were analyzed for Wolbachia infection. The bacteria were genotyped by the MLST and WSP Typing methodologies. All samples were infected with Wolbachia of supergroup “A”. For each of the five MLST genes, unique as well as already known alleles were detected. Nineteen sequence types for the concatenated sequences of the five MLST genes, and twenty wsp alleles were found in the samples. Host-specific haplotypes, shared strains among distinct hosts, and more than one strain of Wolbachia were found in some population samples. Recombination among the MLST genes and intragenic recombination between wsp haplotypes was rare. Phylogenetic analysis showed a great similarity among the Wolbachia strains in the A. fraterculus complex. However, some strains of Wolbachia are found throughout the Neotropical Region and there are specific strains in determined geographical areas. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T16:45:17Z 2018-12-11T16:45:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.syapm.2016.11.002 Systematic and Applied Microbiology, v. 40, n. 1, p. 59-67, 2017. 1618-0984 0723-2020 http://hdl.handle.net/11449/169304 10.1016/j.syapm.2016.11.002 2-s2.0-85007508492 2-s2.0-85007508492.pdf 0165348738208319 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1016/j.syapm.2016.11.002 http://hdl.handle.net/11449/169304 |
identifier_str_mv |
Systematic and Applied Microbiology, v. 40, n. 1, p. 59-67, 2017. 1618-0984 0723-2020 10.1016/j.syapm.2016.11.002 2-s2.0-85007508492 2-s2.0-85007508492.pdf 0165348738208319 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Systematic and Applied Microbiology 1,367 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
59-67 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128365751697408 |