Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle

Detalhes bibliográficos
Autor(a) principal: Pedraza, Francisco J. [UNESP]
Data de Publicação: 2010
Outros Autores: Alessi, Antonio C. [UNESP], Barbosa-Stancioli, Edel F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.org.co/scielo.php?pid=S0120-06902010000300004&script=sci_arttext
http://hdl.handle.net/11449/3028
Resumo: Fifteen cases of viral meningoencephalitis in Colombian cattle were tested by nested PCR analysis for the detection of bovine herpesvirus 5 (BoHV-5). All fatal cases had shown severe neurological signs and had occurred following natural outbreaks of the disease. The neurological infection was histologically characterized by mild to moderate inflammatory changes in the brain and cerebellum, including meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and the presence of Gitter cells (macrophages) accompanying large areas of malacia. No intranuclear inclusion bodies were seen in any of the cases. Results from BoHV-5 molecular extraction analyses showed there were five positive cases thus confirming the presence of the virus in Colombia.
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spelling Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattleDetecção de herpesvírus bovino 5 (BoHV-5) em cérebro incluído em parafina e fixado em formalina, pela PCR, em bovinos colombianosbovine herpesviruspolioencephalomalaciaViral encephalitisFifteen cases of viral meningoencephalitis in Colombian cattle were tested by nested PCR analysis for the detection of bovine herpesvirus 5 (BoHV-5). All fatal cases had shown severe neurological signs and had occurred following natural outbreaks of the disease. The neurological infection was histologically characterized by mild to moderate inflammatory changes in the brain and cerebellum, including meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and the presence of Gitter cells (macrophages) accompanying large areas of malacia. No intranuclear inclusion bodies were seen in any of the cases. Results from BoHV-5 molecular extraction analyses showed there were five positive cases thus confirming the presence of the virus in Colombia.Quinze casos de meningoencefalite viral em gado bovino Colombiano foi analisado pela reação em cadeia da polimerasa (PCR) para a detecção do herpesvírus bovino 5 (BoHV-5). Todos os casos mostraram graves sinais neurológicos antes de morrer y aconteceram como surtos naturais da doença. A infecção neurológica foi caracterizada histologicamente por leves a moderados câmbios inflamatórios no cérebro e cerebelo, incluindo meningite, manguitos perivasculares, gliose, hemorragia e a presencia de células gitter (macrófagos) acompanhando grandes áreas de malacia. Não se encontraram corpos de inclusão intranucleares em nenhum dos casos. Os resultados da análise da extração molecular do BoHV-5 por PCR revelaram a existência de cinco casos positivos, os quais confirmam a presença do vírus na Colômbia.Univ Caldas, Dept Salud Anim, Manizales, ColombiaFCAV UNESP, Jaboticabal, SP, BrazilUniv Estadual Paulista FCAV UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilUniversidade Federal de Minas Gerais (UFMG), Dept Microbiol, Belo Horizonte, MG, BrazilFCAV UNESP, Jaboticabal, SP, BrazilUniv Estadual Paulista FCAV UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilUniv Antioquia, Fac Ciencias AgrariasUniv CaldasUniversidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Pedraza, Francisco J. [UNESP]Alessi, Antonio C. [UNESP]Barbosa-Stancioli, Edel F.2014-05-20T13:16:03Z2014-05-20T13:16:03Z2010-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article292-298http://www.scielo.org.co/scielo.php?pid=S0120-06902010000300004&script=sci_arttextRevista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 23, n. 3, p. 292-298, 2010.0120-0690http://hdl.handle.net/11449/3028S0120-06902010000300004WOS:000284091100004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Colombiana de Ciências Pecuarias0.5160,192info:eu-repo/semantics/openAccess2024-06-07T13:03:18Zoai:repositorio.unesp.br:11449/3028Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:48:56.059045Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
Detecção de herpesvírus bovino 5 (BoHV-5) em cérebro incluído em parafina e fixado em formalina, pela PCR, em bovinos colombianos
title Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
spellingShingle Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
Pedraza, Francisco J. [UNESP]
bovine herpesvirus
polioencephalomalacia
Viral encephalitis
title_short Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
title_full Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
title_fullStr Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
title_full_unstemmed Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
title_sort Detection of bovine herpesvirus 5 (BoHV-5) in formalin-fixed, paraffin-embedded bovine brain by nested PCR in Colombian cattle
author Pedraza, Francisco J. [UNESP]
author_facet Pedraza, Francisco J. [UNESP]
Alessi, Antonio C. [UNESP]
Barbosa-Stancioli, Edel F.
author_role author
author2 Alessi, Antonio C. [UNESP]
Barbosa-Stancioli, Edel F.
author2_role author
author
dc.contributor.none.fl_str_mv Univ Caldas
Universidade Estadual Paulista (Unesp)
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Pedraza, Francisco J. [UNESP]
Alessi, Antonio C. [UNESP]
Barbosa-Stancioli, Edel F.
dc.subject.por.fl_str_mv bovine herpesvirus
polioencephalomalacia
Viral encephalitis
topic bovine herpesvirus
polioencephalomalacia
Viral encephalitis
description Fifteen cases of viral meningoencephalitis in Colombian cattle were tested by nested PCR analysis for the detection of bovine herpesvirus 5 (BoHV-5). All fatal cases had shown severe neurological signs and had occurred following natural outbreaks of the disease. The neurological infection was histologically characterized by mild to moderate inflammatory changes in the brain and cerebellum, including meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and the presence of Gitter cells (macrophages) accompanying large areas of malacia. No intranuclear inclusion bodies were seen in any of the cases. Results from BoHV-5 molecular extraction analyses showed there were five positive cases thus confirming the presence of the virus in Colombia.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
2014-05-20T13:16:03Z
2014-05-20T13:16:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.org.co/scielo.php?pid=S0120-06902010000300004&script=sci_arttext
Revista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 23, n. 3, p. 292-298, 2010.
0120-0690
http://hdl.handle.net/11449/3028
S0120-06902010000300004
WOS:000284091100004
url http://www.scielo.org.co/scielo.php?pid=S0120-06902010000300004&script=sci_arttext
http://hdl.handle.net/11449/3028
identifier_str_mv Revista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 23, n. 3, p. 292-298, 2010.
0120-0690
S0120-06902010000300004
WOS:000284091100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Colombiana de Ciências Pecuarias
0.516
0,192
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 292-298
dc.publisher.none.fl_str_mv Univ Antioquia, Fac Ciencias Agrarias
publisher.none.fl_str_mv Univ Antioquia, Fac Ciencias Agrarias
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129554485608448

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