Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba
Autor(a) principal: | |
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Data de Publicação: | 1980 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jez.1402140110 http://hdl.handle.net/11449/230795 |
Resumo: | Respiratory properties of whole blood and hemoglobin solutions have been studied in the burrowing reptile, Amphisbaena alba. Whole blood is distinguished from that of other squamate reptiles by an extraordinary high O2 affinity (P50 = 12 mmHg at pH 7.60 and 25°C). The Bohr factor,ϕ, was large at −0.85 and the n‐value was 1.80. O2 capacity averaged 12.0 vol%. The molar concentration of erythrocyte ATP was high and twice that of hemoglobin. Stripped Amphisbaena hemoglobin shows an extremely high O2 affinity and reduced pH sensitivity compared to whole blood (P50 = 1 mmHg at pH 7.60 and 25°C, ϕ = −0.35, n‐value = 2.0). The hemoglobin O2 affinity was much more sensitive to ATP than for other poikilotherm vertebrates. Isoelectric focusing revealed a multicomponent hemoglobin with the major components showing similar O2 affinities and Bohr shifts. The data obtained are discussed in relation to the burrowing habits of Amphisbaena and found to be adaptive to a fossorial mode of life. Copyright © 1980 Wiley‐Liss, Inc., A Wiley Company |
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Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena albaRespiratory properties of whole blood and hemoglobin solutions have been studied in the burrowing reptile, Amphisbaena alba. Whole blood is distinguished from that of other squamate reptiles by an extraordinary high O2 affinity (P50 = 12 mmHg at pH 7.60 and 25°C). The Bohr factor,ϕ, was large at −0.85 and the n‐value was 1.80. O2 capacity averaged 12.0 vol%. The molar concentration of erythrocyte ATP was high and twice that of hemoglobin. Stripped Amphisbaena hemoglobin shows an extremely high O2 affinity and reduced pH sensitivity compared to whole blood (P50 = 1 mmHg at pH 7.60 and 25°C, ϕ = −0.35, n‐value = 2.0). The hemoglobin O2 affinity was much more sensitive to ATP than for other poikilotherm vertebrates. Isoelectric focusing revealed a multicomponent hemoglobin with the major components showing similar O2 affinities and Bohr shifts. The data obtained are discussed in relation to the burrowing habits of Amphisbaena and found to be adaptive to a fossorial mode of life. Copyright © 1980 Wiley‐Liss, Inc., A Wiley CompanyDepartment of Zoophysiology University of Aarhus, Aarhus C, DK-8000Department of Zoologia Instituto de Biociências, Rio Claro, São Paulo, CEP 13.500Biology Institute University of Odense, Odense M, DK-5230University of AarhusInstituto de BiociênciasUniversity of OdenseJohansen, K.Abe, A. S.Weber, R. E.2022-04-29T08:42:01Z2022-04-29T08:42:01Z1980-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article71-77http://dx.doi.org/10.1002/jez.1402140110Journal of Experimental Zoology, v. 214, n. 1, p. 71-77, 1980.1097-010X0022-104Xhttp://hdl.handle.net/11449/23079510.1002/jez.14021401102-s2.0-0019075748Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Experimental Zoologyinfo:eu-repo/semantics/openAccess2022-04-29T08:42:01Zoai:repositorio.unesp.br:11449/230795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:22:17.235574Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
title |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
spellingShingle |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba Johansen, K. |
title_short |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
title_full |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
title_fullStr |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
title_full_unstemmed |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
title_sort |
Respiratory properties of whole blood and hemoglobin from the burrowing reptile, Amphisbaena alba |
author |
Johansen, K. |
author_facet |
Johansen, K. Abe, A. S. Weber, R. E. |
author_role |
author |
author2 |
Abe, A. S. Weber, R. E. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
University of Aarhus Instituto de Biociências University of Odense |
dc.contributor.author.fl_str_mv |
Johansen, K. Abe, A. S. Weber, R. E. |
description |
Respiratory properties of whole blood and hemoglobin solutions have been studied in the burrowing reptile, Amphisbaena alba. Whole blood is distinguished from that of other squamate reptiles by an extraordinary high O2 affinity (P50 = 12 mmHg at pH 7.60 and 25°C). The Bohr factor,ϕ, was large at −0.85 and the n‐value was 1.80. O2 capacity averaged 12.0 vol%. The molar concentration of erythrocyte ATP was high and twice that of hemoglobin. Stripped Amphisbaena hemoglobin shows an extremely high O2 affinity and reduced pH sensitivity compared to whole blood (P50 = 1 mmHg at pH 7.60 and 25°C, ϕ = −0.35, n‐value = 2.0). The hemoglobin O2 affinity was much more sensitive to ATP than for other poikilotherm vertebrates. Isoelectric focusing revealed a multicomponent hemoglobin with the major components showing similar O2 affinities and Bohr shifts. The data obtained are discussed in relation to the burrowing habits of Amphisbaena and found to be adaptive to a fossorial mode of life. Copyright © 1980 Wiley‐Liss, Inc., A Wiley Company |
publishDate |
1980 |
dc.date.none.fl_str_mv |
1980-01-01 2022-04-29T08:42:01Z 2022-04-29T08:42:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jez.1402140110 Journal of Experimental Zoology, v. 214, n. 1, p. 71-77, 1980. 1097-010X 0022-104X http://hdl.handle.net/11449/230795 10.1002/jez.1402140110 2-s2.0-0019075748 |
url |
http://dx.doi.org/10.1002/jez.1402140110 http://hdl.handle.net/11449/230795 |
identifier_str_mv |
Journal of Experimental Zoology, v. 214, n. 1, p. 71-77, 1980. 1097-010X 0022-104X 10.1002/jez.1402140110 2-s2.0-0019075748 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Experimental Zoology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
71-77 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128221350199296 |