The transcriptome of Cryptosporidium oocysts and intracellular stages
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-019-44289-x http://hdl.handle.net/11449/187703 |
Resumo: | Human cryptosporidiosis is caused primarily by two species of apicomplexan parasites, Cryptosporidium parvum and C. hominis. Although infection of cell monolayers with sporozoites does not support the complete parasite life cycle, the in vitro system is used to study the asexual phase of multiplication, which consists of two generations of merogony. To better understand host-parasite interaction and to gain insight into gene regulatory processes driving the complex life cycle of Cryptosporidium parasites, we analyzed the transcriptome of C. parvum in oocysts, sporozoites and infected cell monolayers 2–48 h post-infection. Analysis of RNA-Seq data from replicate oocyst, sporozoite and intracellular samples revealed significant differences between transcriptomes expressed outside and inside the host cell. Compared to the transcriptome found in the host cell, the oocyst transcriptome is less diverse. Biological processes significantly over-represented intracellularly relate to biosynthetic processes. Genes significantly overexpressed in oocysts show evidence of specialized functions not found in other Apicomplexa. A more comprehensive view of gene regulation during the Cryptosporidium life cycle will require the analysis of later time points during the infection, particularly of the poorly studied sexual phase of the life cycle. |
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The transcriptome of Cryptosporidium oocysts and intracellular stagesHuman cryptosporidiosis is caused primarily by two species of apicomplexan parasites, Cryptosporidium parvum and C. hominis. Although infection of cell monolayers with sporozoites does not support the complete parasite life cycle, the in vitro system is used to study the asexual phase of multiplication, which consists of two generations of merogony. To better understand host-parasite interaction and to gain insight into gene regulatory processes driving the complex life cycle of Cryptosporidium parasites, we analyzed the transcriptome of C. parvum in oocysts, sporozoites and infected cell monolayers 2–48 h post-infection. Analysis of RNA-Seq data from replicate oocyst, sporozoite and intracellular samples revealed significant differences between transcriptomes expressed outside and inside the host cell. Compared to the transcriptome found in the host cell, the oocyst transcriptome is less diverse. Biological processes significantly over-represented intracellularly relate to biosynthetic processes. Genes significantly overexpressed in oocysts show evidence of specialized functions not found in other Apicomplexa. A more comprehensive view of gene regulation during the Cryptosporidium life cycle will require the analysis of later time points during the infection, particularly of the poorly studied sexual phase of the life cycle.National Institute of Allergy and Infectious DiseasesDivision of Intramural Research, National Institute of Allergy and Infectious DiseasesDepartment of Infectious Disease & Global Health Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias JaboticabalDepartment of Microbiological Sciences North Dakota State UniversityUniversidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária AraçatubaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias JaboticabalUniversidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária AraçatubaNational Institute of Allergy and Infectious Diseases: 1 R15 AI122152-01A1Division of Intramural Research, National Institute of Allergy and Infectious Diseases: R15 AI122152Division of Intramural Research, National Institute of Allergy and Infectious Diseases: U19 AI09776Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual Paulista (Unesp)North Dakota State UniversityMatos, Lucas V. S. [UNESP]McEvoy, JohnTzipori, SaulBresciani, Katia D. S. [UNESP]Widmer, Giovanni2019-10-06T15:44:41Z2019-10-06T15:44:41Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-019-44289-xScientific Reports, v. 9, n. 1, 2019.2045-2322http://hdl.handle.net/11449/18770310.1038/s41598-019-44289-x2-s2.0-85066280450Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T09:20:06Zoai:repositorio.unesp.br:11449/187703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:58:40.922014Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
title |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
spellingShingle |
The transcriptome of Cryptosporidium oocysts and intracellular stages Matos, Lucas V. S. [UNESP] |
title_short |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
title_full |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
title_fullStr |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
title_full_unstemmed |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
title_sort |
The transcriptome of Cryptosporidium oocysts and intracellular stages |
author |
Matos, Lucas V. S. [UNESP] |
author_facet |
Matos, Lucas V. S. [UNESP] McEvoy, John Tzipori, Saul Bresciani, Katia D. S. [UNESP] Widmer, Giovanni |
author_role |
author |
author2 |
McEvoy, John Tzipori, Saul Bresciani, Katia D. S. [UNESP] Widmer, Giovanni |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Cummings School of Veterinary Medicine at Tufts University Universidade Estadual Paulista (Unesp) North Dakota State University |
dc.contributor.author.fl_str_mv |
Matos, Lucas V. S. [UNESP] McEvoy, John Tzipori, Saul Bresciani, Katia D. S. [UNESP] Widmer, Giovanni |
description |
Human cryptosporidiosis is caused primarily by two species of apicomplexan parasites, Cryptosporidium parvum and C. hominis. Although infection of cell monolayers with sporozoites does not support the complete parasite life cycle, the in vitro system is used to study the asexual phase of multiplication, which consists of two generations of merogony. To better understand host-parasite interaction and to gain insight into gene regulatory processes driving the complex life cycle of Cryptosporidium parasites, we analyzed the transcriptome of C. parvum in oocysts, sporozoites and infected cell monolayers 2–48 h post-infection. Analysis of RNA-Seq data from replicate oocyst, sporozoite and intracellular samples revealed significant differences between transcriptomes expressed outside and inside the host cell. Compared to the transcriptome found in the host cell, the oocyst transcriptome is less diverse. Biological processes significantly over-represented intracellularly relate to biosynthetic processes. Genes significantly overexpressed in oocysts show evidence of specialized functions not found in other Apicomplexa. A more comprehensive view of gene regulation during the Cryptosporidium life cycle will require the analysis of later time points during the infection, particularly of the poorly studied sexual phase of the life cycle. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:44:41Z 2019-10-06T15:44:41Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-019-44289-x Scientific Reports, v. 9, n. 1, 2019. 2045-2322 http://hdl.handle.net/11449/187703 10.1038/s41598-019-44289-x 2-s2.0-85066280450 |
url |
http://dx.doi.org/10.1038/s41598-019-44289-x http://hdl.handle.net/11449/187703 |
identifier_str_mv |
Scientific Reports, v. 9, n. 1, 2019. 2045-2322 10.1038/s41598-019-44289-x 2-s2.0-85066280450 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129568792379392 |