Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Detalhes bibliográficos
Autor(a) principal: Porfirio, Grasiela Edith de Oliveira
Data de Publicação: 2018
Outros Autores: Santos, Filipe Martins, de Macedo, Gabriel Carvalho, Barreto, Wanessa Teixeira Gomes, Campos, João Bosco Vilela, Meyers, Alyssa C., André, Marcos Rogério [UNESP], Perles, Lívia [UNESP], de Oliveira, Carina Elisei, Xavier, Samanta Cristina das Chagas, Andrade, Gisele Braziliano de, Jansen, Ana Maria, Herrera, Heitor Miraglia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ijppaw.2018.10.004
http://hdl.handle.net/11449/186983
Resumo: Domestic dogs are considered reservoirs hosts for several vector-borne parasites. This study aimed to evaluate the role of domestic dogs as hosts for Trypanosoma cruzi, Trypanosoma evansi and Leishmania spp. in single and co-infections in the Urucum settlement, near the Brazil-Bolivian border. Additionally, we evaluated the involvement of wild mammals’ in the maintenance of these parasites in the study area. Blood samples of dogs (n = 62) and six species of wild mammals (n = 36) were collected in July and August of 2015. The infections were assessed using parasitological, serological and molecular tests. Clinical examination of dogs was performed and their feeding habits were noted. Overall, 87% (54/62) of sampled dogs were positive for at least one trypanosomatid species, in single (n = 9) and co-infections (n = 45). We found that 76% of dogs were positive for T. cruzi, four of them displayed high parasitemias demonstrated by hemoculture, including one strain types TcI, two TcIII and one TcIII/TcV. Around 73% (45/62) of dogs were positive to T. evansi, three with high parasitemias as seen by positive microhematocrit centrifuge technique. Of dogs sampled, 50% (31/62) were positive for Leishmania spp. by PCR or serology. We found a positive influence of (i) T. evansi on mucous pallor, (ii) co-infection by T. cruzi and Leishmania with onychogryphosis, and (iii) all parasites to skin lesions of sampled dogs. Finally, feeding on wild mammals had a positive influence in the Leishmania spp. infection in dogs. We found that 28% (5/18) coati Nasua nasua was co-infected for all three trypanosamatids, demonstrating that it might play a key role in maintenance of these parasites. Our results showed the importance of Urucum region as a hotspot for T. cruzi, T. evansi and Leishmania spp. and demonstrated that dogs can be considered as incidental hosts.
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spelling Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian borderCanineNeglected diseasesPantanalSentinels hostsTrypanosomatidsDomestic dogs are considered reservoirs hosts for several vector-borne parasites. This study aimed to evaluate the role of domestic dogs as hosts for Trypanosoma cruzi, Trypanosoma evansi and Leishmania spp. in single and co-infections in the Urucum settlement, near the Brazil-Bolivian border. Additionally, we evaluated the involvement of wild mammals’ in the maintenance of these parasites in the study area. Blood samples of dogs (n = 62) and six species of wild mammals (n = 36) were collected in July and August of 2015. The infections were assessed using parasitological, serological and molecular tests. Clinical examination of dogs was performed and their feeding habits were noted. Overall, 87% (54/62) of sampled dogs were positive for at least one trypanosomatid species, in single (n = 9) and co-infections (n = 45). We found that 76% of dogs were positive for T. cruzi, four of them displayed high parasitemias demonstrated by hemoculture, including one strain types TcI, two TcIII and one TcIII/TcV. Around 73% (45/62) of dogs were positive to T. evansi, three with high parasitemias as seen by positive microhematocrit centrifuge technique. Of dogs sampled, 50% (31/62) were positive for Leishmania spp. by PCR or serology. We found a positive influence of (i) T. evansi on mucous pallor, (ii) co-infection by T. cruzi and Leishmania with onychogryphosis, and (iii) all parasites to skin lesions of sampled dogs. Finally, feeding on wild mammals had a positive influence in the Leishmania spp. infection in dogs. We found that 28% (5/18) coati Nasua nasua was co-infected for all three trypanosamatids, demonstrating that it might play a key role in maintenance of these parasites. Our results showed the importance of Urucum region as a hotspot for T. cruzi, T. evansi and Leishmania spp. and demonstrated that dogs can be considered as incidental hosts.Programa de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária Universidade Católica Dom Bosco, Tamandaré Avenue, 6000. Jardim SeminárioPrograma de Pós-Graduação em Ecologia e Conservação Universidade Federal de Mato Grosso do Sul, Costa e Silva AvenueDepartment of Veterinary Integrative Biosciences Texas A&M University, 402 Raymond Stotzer ParkwayUniversidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Prof. Paulo Donato Castelane StreetLaboratório de Biologia de Tripanosomatídeos Instituto Oswaldo Cruz Fundação Oswaldo Cruz, Brazil Avenue, ManguinhosUniversidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, Prof. Paulo Donato Castelane StreetUniversidade Católica Dom BoscoUniversidade Federal de Mato Grosso do Sul (UFMS)Texas A&M UniversityUniversidade Estadual Paulista (Unesp)Fundação Oswaldo CruzPorfirio, Grasiela Edith de OliveiraSantos, Filipe Martinsde Macedo, Gabriel CarvalhoBarreto, Wanessa Teixeira GomesCampos, João Bosco VilelaMeyers, Alyssa C.André, Marcos Rogério [UNESP]Perles, Lívia [UNESP]de Oliveira, Carina EliseiXavier, Samanta Cristina das ChagasAndrade, Gisele Braziliano deJansen, Ana MariaHerrera, Heitor Miraglia2019-10-06T15:21:54Z2019-10-06T15:21:54Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article398-404http://dx.doi.org/10.1016/j.ijppaw.2018.10.004International Journal for Parasitology: Parasites and Wildlife, v. 7, n. 3, p. 398-404, 2018.2213-2244http://hdl.handle.net/11449/18698310.1016/j.ijppaw.2018.10.0042-s2.0-85055162340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal for Parasitology: Parasites and Wildlifeinfo:eu-repo/semantics/openAccess2021-10-23T12:23:56Zoai:repositorio.unesp.br:11449/186983Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:23:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
title Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
spellingShingle Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
Porfirio, Grasiela Edith de Oliveira
Canine
Neglected diseases
Pantanal
Sentinels hosts
Trypanosomatids
title_short Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
title_full Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
title_fullStr Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
title_full_unstemmed Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
title_sort Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border
author Porfirio, Grasiela Edith de Oliveira
author_facet Porfirio, Grasiela Edith de Oliveira
Santos, Filipe Martins
de Macedo, Gabriel Carvalho
Barreto, Wanessa Teixeira Gomes
Campos, João Bosco Vilela
Meyers, Alyssa C.
André, Marcos Rogério [UNESP]
Perles, Lívia [UNESP]
de Oliveira, Carina Elisei
Xavier, Samanta Cristina das Chagas
Andrade, Gisele Braziliano de
Jansen, Ana Maria
Herrera, Heitor Miraglia
author_role author
author2 Santos, Filipe Martins
de Macedo, Gabriel Carvalho
Barreto, Wanessa Teixeira Gomes
Campos, João Bosco Vilela
Meyers, Alyssa C.
André, Marcos Rogério [UNESP]
Perles, Lívia [UNESP]
de Oliveira, Carina Elisei
Xavier, Samanta Cristina das Chagas
Andrade, Gisele Braziliano de
Jansen, Ana Maria
Herrera, Heitor Miraglia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Católica Dom Bosco
Universidade Federal de Mato Grosso do Sul (UFMS)
Texas A&M University
Universidade Estadual Paulista (Unesp)
Fundação Oswaldo Cruz
dc.contributor.author.fl_str_mv Porfirio, Grasiela Edith de Oliveira
Santos, Filipe Martins
de Macedo, Gabriel Carvalho
Barreto, Wanessa Teixeira Gomes
Campos, João Bosco Vilela
Meyers, Alyssa C.
André, Marcos Rogério [UNESP]
Perles, Lívia [UNESP]
de Oliveira, Carina Elisei
Xavier, Samanta Cristina das Chagas
Andrade, Gisele Braziliano de
Jansen, Ana Maria
Herrera, Heitor Miraglia
dc.subject.por.fl_str_mv Canine
Neglected diseases
Pantanal
Sentinels hosts
Trypanosomatids
topic Canine
Neglected diseases
Pantanal
Sentinels hosts
Trypanosomatids
description Domestic dogs are considered reservoirs hosts for several vector-borne parasites. This study aimed to evaluate the role of domestic dogs as hosts for Trypanosoma cruzi, Trypanosoma evansi and Leishmania spp. in single and co-infections in the Urucum settlement, near the Brazil-Bolivian border. Additionally, we evaluated the involvement of wild mammals’ in the maintenance of these parasites in the study area. Blood samples of dogs (n = 62) and six species of wild mammals (n = 36) were collected in July and August of 2015. The infections were assessed using parasitological, serological and molecular tests. Clinical examination of dogs was performed and their feeding habits were noted. Overall, 87% (54/62) of sampled dogs were positive for at least one trypanosomatid species, in single (n = 9) and co-infections (n = 45). We found that 76% of dogs were positive for T. cruzi, four of them displayed high parasitemias demonstrated by hemoculture, including one strain types TcI, two TcIII and one TcIII/TcV. Around 73% (45/62) of dogs were positive to T. evansi, three with high parasitemias as seen by positive microhematocrit centrifuge technique. Of dogs sampled, 50% (31/62) were positive for Leishmania spp. by PCR or serology. We found a positive influence of (i) T. evansi on mucous pallor, (ii) co-infection by T. cruzi and Leishmania with onychogryphosis, and (iii) all parasites to skin lesions of sampled dogs. Finally, feeding on wild mammals had a positive influence in the Leishmania spp. infection in dogs. We found that 28% (5/18) coati Nasua nasua was co-infected for all three trypanosamatids, demonstrating that it might play a key role in maintenance of these parasites. Our results showed the importance of Urucum region as a hotspot for T. cruzi, T. evansi and Leishmania spp. and demonstrated that dogs can be considered as incidental hosts.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2019-10-06T15:21:54Z
2019-10-06T15:21:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ijppaw.2018.10.004
International Journal for Parasitology: Parasites and Wildlife, v. 7, n. 3, p. 398-404, 2018.
2213-2244
http://hdl.handle.net/11449/186983
10.1016/j.ijppaw.2018.10.004
2-s2.0-85055162340
url http://dx.doi.org/10.1016/j.ijppaw.2018.10.004
http://hdl.handle.net/11449/186983
identifier_str_mv International Journal for Parasitology: Parasites and Wildlife, v. 7, n. 3, p. 398-404, 2018.
2213-2244
10.1016/j.ijppaw.2018.10.004
2-s2.0-85055162340
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal for Parasitology: Parasites and Wildlife
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 398-404
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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