Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0014168 http://hdl.handle.net/11449/42347 |
Resumo: | The observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development. |
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Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal DevelopmentThe observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development.Katholieke Universiteit LeuvenFund for Scientific Research Flanders (FWO)Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)Katholieke Univ Leuven, Lab Expt Med & Endocrinol, Louvain, BelgiumSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilMRC, Ctr Reprod Biol, Human Reprod Sci Unit, Edinburgh, Midlothian, ScotlandUniv Fed Minas Gerais, Lab Cellular Biol, Dept Morphol, Inst Biol Sci, Belo Horizonte, MG, BrazilSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilKatholieke Universiteit Leuven: OT/07/068AFund for Scientific Research Flanders (FWO): G.0458.05NInstitute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen): SB-73064Public Library ScienceKatholieke Univ LeuvenUniversidade Estadual Paulista (Unesp)MRCUniversidade Federal de Minas Gerais (UFMG)Willems, ArianeBatlouni, Sergio R. [UNESP]Esnal, ArantzaSwinnen, Johannes V.Saunders, Philippa T. K.Sharpe, Richard M.Franca, Luiz R.De Gendt, KarelVerhoeven, Guido2014-05-20T15:33:53Z2014-05-20T15:33:53Z2010-11-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1371/journal.pone.0014168Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010.1932-6203http://hdl.handle.net/11449/4234710.1371/journal.pone.0014168WOS:000284755100045WOS000284755100045.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-04-09T15:37:12Zoai:repositorio.unesp.br:11449/42347Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:20.285447Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
title |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
spellingShingle |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development Willems, Ariane |
title_short |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
title_full |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
title_fullStr |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
title_full_unstemmed |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
title_sort |
Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development |
author |
Willems, Ariane |
author_facet |
Willems, Ariane Batlouni, Sergio R. [UNESP] Esnal, Arantza Swinnen, Johannes V. Saunders, Philippa T. K. Sharpe, Richard M. Franca, Luiz R. De Gendt, Karel Verhoeven, Guido |
author_role |
author |
author2 |
Batlouni, Sergio R. [UNESP] Esnal, Arantza Swinnen, Johannes V. Saunders, Philippa T. K. Sharpe, Richard M. Franca, Luiz R. De Gendt, Karel Verhoeven, Guido |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Katholieke Univ Leuven Universidade Estadual Paulista (Unesp) MRC Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Willems, Ariane Batlouni, Sergio R. [UNESP] Esnal, Arantza Swinnen, Johannes V. Saunders, Philippa T. K. Sharpe, Richard M. Franca, Luiz R. De Gendt, Karel Verhoeven, Guido |
description |
The observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-30 2014-05-20T15:33:53Z 2014-05-20T15:33:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0014168 Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010. 1932-6203 http://hdl.handle.net/11449/42347 10.1371/journal.pone.0014168 WOS:000284755100045 WOS000284755100045.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0014168 http://hdl.handle.net/11449/42347 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010. 1932-6203 10.1371/journal.pone.0014168 WOS:000284755100045 WOS000284755100045.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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PLOS ONE 2.766 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129296158425088 |