Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development

Detalhes bibliográficos
Autor(a) principal: Willems, Ariane
Data de Publicação: 2010
Outros Autores: Batlouni, Sergio R. [UNESP], Esnal, Arantza, Swinnen, Johannes V., Saunders, Philippa T. K., Sharpe, Richard M., Franca, Luiz R., De Gendt, Karel, Verhoeven, Guido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0014168
http://hdl.handle.net/11449/42347
Resumo: The observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development.
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spelling Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal DevelopmentThe observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development.Katholieke Universiteit LeuvenFund for Scientific Research Flanders (FWO)Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)Katholieke Univ Leuven, Lab Expt Med & Endocrinol, Louvain, BelgiumSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilMRC, Ctr Reprod Biol, Human Reprod Sci Unit, Edinburgh, Midlothian, ScotlandUniv Fed Minas Gerais, Lab Cellular Biol, Dept Morphol, Inst Biol Sci, Belo Horizonte, MG, BrazilSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilKatholieke Universiteit Leuven: OT/07/068AFund for Scientific Research Flanders (FWO): G.0458.05NInstitute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen): SB-73064Public Library ScienceKatholieke Univ LeuvenUniversidade Estadual Paulista (Unesp)MRCUniversidade Federal de Minas Gerais (UFMG)Willems, ArianeBatlouni, Sergio R. [UNESP]Esnal, ArantzaSwinnen, Johannes V.Saunders, Philippa T. K.Sharpe, Richard M.Franca, Luiz R.De Gendt, KarelVerhoeven, Guido2014-05-20T15:33:53Z2014-05-20T15:33:53Z2010-11-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1371/journal.pone.0014168Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010.1932-6203http://hdl.handle.net/11449/4234710.1371/journal.pone.0014168WOS:000284755100045WOS000284755100045.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-04-09T15:37:12Zoai:repositorio.unesp.br:11449/42347Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:20.285447Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
title Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
spellingShingle Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
Willems, Ariane
title_short Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
title_full Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
title_fullStr Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
title_full_unstemmed Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
title_sort Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
author Willems, Ariane
author_facet Willems, Ariane
Batlouni, Sergio R. [UNESP]
Esnal, Arantza
Swinnen, Johannes V.
Saunders, Philippa T. K.
Sharpe, Richard M.
Franca, Luiz R.
De Gendt, Karel
Verhoeven, Guido
author_role author
author2 Batlouni, Sergio R. [UNESP]
Esnal, Arantza
Swinnen, Johannes V.
Saunders, Philippa T. K.
Sharpe, Richard M.
Franca, Luiz R.
De Gendt, Karel
Verhoeven, Guido
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Katholieke Univ Leuven
Universidade Estadual Paulista (Unesp)
MRC
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Willems, Ariane
Batlouni, Sergio R. [UNESP]
Esnal, Arantza
Swinnen, Johannes V.
Saunders, Philippa T. K.
Sharpe, Richard M.
Franca, Luiz R.
De Gendt, Karel
Verhoeven, Guido
description The observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development.
publishDate 2010
dc.date.none.fl_str_mv 2010-11-30
2014-05-20T15:33:53Z
2014-05-20T15:33:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0014168
Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010.
1932-6203
http://hdl.handle.net/11449/42347
10.1371/journal.pone.0014168
WOS:000284755100045
WOS000284755100045.pdf
url http://dx.doi.org/10.1371/journal.pone.0014168
http://hdl.handle.net/11449/42347
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 5, n. 11, p. 16, 2010.
1932-6203
10.1371/journal.pone.0014168
WOS:000284755100045
WOS000284755100045.pdf
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publisher.none.fl_str_mv Public Library Science
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