Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/224454 |
Resumo: | Eighty broiler chicks, Naked neck (Na/na) and Hubbard-Pettersen strains, were exposed to acute heat stress (36-37°C), from 2 to 5 days of age, and body weight and cloacal temperature were measured in the beginning and at the end of the heat stress period (5 h). Birds were sacrificed at the end and liver and brain samples were collected and analyzed using Western Blotting and Northern Blotting to quantify Hsp70 levels and mRNA Hsp70 transcript, respectively. The brain tissue had higher Hsp70 level than liver tissue. Before heat stress it was not observed difference in the Hsp70 expression between strains. After heat stress in the liver it was observed in increase in the Hsp70 level (naked neck birds at 4 and 5 days), and in the brain a reduced Hsp70 level was found when compared with the control birds (Naked neck birds at 2 days). The size of the mRNA Hsp70 transcript was 2.7 kb. The data of this experiment suggest that to the brain tissue heat stress affect Hsp70 at transcriptional and translational levels, and for hepatic tissue the effect is only at translational level, for different lines. |
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Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes LinhagensEffect of Acute Heat Stress on Hepatic and Cerebral Messenger RNA Heat Shock Protein 70 and Heat Shock Protein 70 Level of Broiler Chicks from 2 to 5 Days Old of Different StrainsAcute heat stressHepatic and brain Hsp70Northern Blotting and Western BlottingProtein and mRNA Hsp70Eighty broiler chicks, Naked neck (Na/na) and Hubbard-Pettersen strains, were exposed to acute heat stress (36-37°C), from 2 to 5 days of age, and body weight and cloacal temperature were measured in the beginning and at the end of the heat stress period (5 h). Birds were sacrificed at the end and liver and brain samples were collected and analyzed using Western Blotting and Northern Blotting to quantify Hsp70 levels and mRNA Hsp70 transcript, respectively. The brain tissue had higher Hsp70 level than liver tissue. Before heat stress it was not observed difference in the Hsp70 expression between strains. After heat stress in the liver it was observed in increase in the Hsp70 level (naked neck birds at 4 and 5 days), and in the brain a reduced Hsp70 level was found when compared with the control birds (Naked neck birds at 2 days). The size of the mRNA Hsp70 transcript was 2.7 kb. The data of this experiment suggest that to the brain tissue heat stress affect Hsp70 at transcriptional and translational levels, and for hepatic tissue the effect is only at translational level, for different lines.Prog. Pos Graduacao em Biotecnologia UFPelDepartamento de TecnologiaDepto. de Morfol. e Fisiol. Animal Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista-UNESP, Jaboticabal, SP. 14870-000Departamento de Zootecnia Faculdade de Agronomia Eliseu Maciel Univ. Federal de Pelotas-UFPel, Pelotas, RS. 96100-000Depto. de Melhoramento e Nutr. Anim. FMVZ-UNESP, Botucatu, SPDepto. de Morfol. e Fisiol. Animal Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista-UNESP, Jaboticabal, SP. 14870-000Depto. de Melhoramento e Nutr. Anim. FMVZ-UNESP, Botucatu, SPUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (UNESP)Dionello, Nelson José LaurinoFerro, Jesus AparecidoMacari, Marcos [UNESP]Rutz, FernandoFurlan, Luiz Roberto [UNESP]Ferro, Maria Inês Tiraboschi2022-04-28T19:56:36Z2022-04-28T19:56:36Z2001-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1506-1513Revista Brasileira de Zootecnia, v. 30, n. 5, p. 1506-1513, 2001.0100-4859http://hdl.handle.net/11449/2244542-s2.0-0347670358Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Zootecniainfo:eu-repo/semantics/openAccess2024-09-06T18:54:56Zoai:repositorio.unesp.br:11449/224454Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:54:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens Effect of Acute Heat Stress on Hepatic and Cerebral Messenger RNA Heat Shock Protein 70 and Heat Shock Protein 70 Level of Broiler Chicks from 2 to 5 Days Old of Different Strains |
title |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
spellingShingle |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens Dionello, Nelson José Laurino Acute heat stress Hepatic and brain Hsp70 Northern Blotting and Western Blotting Protein and mRNA Hsp70 |
title_short |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
title_full |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
title_fullStr |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
title_full_unstemmed |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
title_sort |
Efeito do Estresse Térmico Agudo sobre os Níveis da Proteína e RNA Mensageiro da HSP70, em Fígado e Cérebro de Pintos de Corte de Diferentes Linhagens |
author |
Dionello, Nelson José Laurino |
author_facet |
Dionello, Nelson José Laurino Ferro, Jesus Aparecido Macari, Marcos [UNESP] Rutz, Fernando Furlan, Luiz Roberto [UNESP] Ferro, Maria Inês Tiraboschi |
author_role |
author |
author2 |
Ferro, Jesus Aparecido Macari, Marcos [UNESP] Rutz, Fernando Furlan, Luiz Roberto [UNESP] Ferro, Maria Inês Tiraboschi |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Dionello, Nelson José Laurino Ferro, Jesus Aparecido Macari, Marcos [UNESP] Rutz, Fernando Furlan, Luiz Roberto [UNESP] Ferro, Maria Inês Tiraboschi |
dc.subject.por.fl_str_mv |
Acute heat stress Hepatic and brain Hsp70 Northern Blotting and Western Blotting Protein and mRNA Hsp70 |
topic |
Acute heat stress Hepatic and brain Hsp70 Northern Blotting and Western Blotting Protein and mRNA Hsp70 |
description |
Eighty broiler chicks, Naked neck (Na/na) and Hubbard-Pettersen strains, were exposed to acute heat stress (36-37°C), from 2 to 5 days of age, and body weight and cloacal temperature were measured in the beginning and at the end of the heat stress period (5 h). Birds were sacrificed at the end and liver and brain samples were collected and analyzed using Western Blotting and Northern Blotting to quantify Hsp70 levels and mRNA Hsp70 transcript, respectively. The brain tissue had higher Hsp70 level than liver tissue. Before heat stress it was not observed difference in the Hsp70 expression between strains. After heat stress in the liver it was observed in increase in the Hsp70 level (naked neck birds at 4 and 5 days), and in the brain a reduced Hsp70 level was found when compared with the control birds (Naked neck birds at 2 days). The size of the mRNA Hsp70 transcript was 2.7 kb. The data of this experiment suggest that to the brain tissue heat stress affect Hsp70 at transcriptional and translational levels, and for hepatic tissue the effect is only at translational level, for different lines. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-09-01 2022-04-28T19:56:36Z 2022-04-28T19:56:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Zootecnia, v. 30, n. 5, p. 1506-1513, 2001. 0100-4859 http://hdl.handle.net/11449/224454 2-s2.0-0347670358 |
identifier_str_mv |
Revista Brasileira de Zootecnia, v. 30, n. 5, p. 1506-1513, 2001. 0100-4859 2-s2.0-0347670358 |
url |
http://hdl.handle.net/11449/224454 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Zootecnia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1506-1513 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021322549362688 |