Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5935/0004-2749.20150098 http://hdl.handle.net/11449/177703 |
Resumo: | Purpose: To evaluate the effects of 1% morphine instillation on clinical parameters, aqueous humor turbidity, and expression levels of tumor necrosis factor alpha (TNF-a), interleukin-1 beta (IL-1beta), prostaglandin E2 (PGE2), and myeloperoxidase (MPO) in rabbits with endotoxin-induced experimental uveitis. Methods: Twenty four New Zealand white rabbits were divided into four groups (n=6 each): control (CG), morphine (MG), naloxone (NG), and morphine-naloxone (MNG) groups. Under dissociative anesthesia, 0.1 mL of solution containing 0.2 μg of lipopolysaccharide (LPS) endotoxin from the Salmonella typhimurium cell wall was injected in the vitreous chamber. Clinical evaluations (conjunctical hyperemia, chemosis blepharospasm, and ocular discharge) and laser flaremetry were performed before (baseline), and 10 and 20 hours after induction of uveitis. Rabbits were subsequently euthanized and eyes were enucleated to quantify expression levels of TNF-α, IL-1 beta, PGE2, and MPO. Results: No significant differences in clinical parameters and flare values were observed between the study groups. TNF-α and IL-1 beta levels increased significantly in the CG, MG, NG, and MNG groups compared to baseline (P<0.05). Significant differences in PGE2 levels were observed between the MG and NMG groups (P<0.05). A trend toward increased MPO activity was observed in response to uveitis induction; however, this trend did not reach statistical significance (P>0.05). Conclusions: Morphine has no effect on clinical parameters, flare, or expression levels of inflammatory mediators in a rabbit model of uveitis induced by intravitreal injection of LPS. |
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Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitisEndotoxins/toxicityLasers/diagnostic useMorphine/administration&dosagePhotmetry/methodsRabbitUveitis/chemically inducedPurpose: To evaluate the effects of 1% morphine instillation on clinical parameters, aqueous humor turbidity, and expression levels of tumor necrosis factor alpha (TNF-a), interleukin-1 beta (IL-1beta), prostaglandin E2 (PGE2), and myeloperoxidase (MPO) in rabbits with endotoxin-induced experimental uveitis. Methods: Twenty four New Zealand white rabbits were divided into four groups (n=6 each): control (CG), morphine (MG), naloxone (NG), and morphine-naloxone (MNG) groups. Under dissociative anesthesia, 0.1 mL of solution containing 0.2 μg of lipopolysaccharide (LPS) endotoxin from the Salmonella typhimurium cell wall was injected in the vitreous chamber. Clinical evaluations (conjunctical hyperemia, chemosis blepharospasm, and ocular discharge) and laser flaremetry were performed before (baseline), and 10 and 20 hours after induction of uveitis. Rabbits were subsequently euthanized and eyes were enucleated to quantify expression levels of TNF-α, IL-1 beta, PGE2, and MPO. Results: No significant differences in clinical parameters and flare values were observed between the study groups. TNF-α and IL-1 beta levels increased significantly in the CG, MG, NG, and MNG groups compared to baseline (P<0.05). Significant differences in PGE2 levels were observed between the MG and NMG groups (P<0.05). A trend toward increased MPO activity was observed in response to uveitis induction; however, this trend did not reach statistical significance (P>0.05). Conclusions: Morphine has no effect on clinical parameters, flare, or expression levels of inflammatory mediators in a rabbit model of uveitis induced by intravitreal injection of LPS.Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal Universidade Estadual Paulista Julio de Mesquita Filho (UNESP)Departament of Veterinary Clinical Medicine Faculdade de Agronomia Medicina Veterinária e Zootecnia Universidade Federal de Mato Grosso (UFMG)Instituto de Ciências Biomédicas Universidade Federal de Uberlândia (UFU)Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal Universidade Estadual Paulista Julio de Mesquita Filho (UNESP)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Universidade Federal de Uberlândia (UFU)Ortencio, Kethye P. [UNESP]Renzo, Roberta [UNESP]Sobrinho, Alexandre A.F.B. [UNESP]Kobashigawa, Karina Kamachi [UNESP]Crivelaro, Roberta M. [UNESP]Silva, Germana A. [UNESP]Aldrovani, Marcela [UNESP]Ribeiro, Alexandre PintoMineo, Tiago W.P.Laus, José Luiz [UNESP]2018-12-11T17:26:42Z2018-12-11T17:26:42Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article371-375application/pdfhttp://dx.doi.org/10.5935/0004-2749.20150098Arquivos Brasileiros de Oftalmologia, v. 78, n. 6, p. 371-375, 2015.1678-29250004-2749http://hdl.handle.net/11449/17770310.5935/0004-2749.201500982-s2.0-849537267102-s2.0-84953726710.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Oftalmologia0,518info:eu-repo/semantics/openAccess2023-11-14T06:17:31Zoai:repositorio.unesp.br:11449/177703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-14T06:17:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
title |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
spellingShingle |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis Ortencio, Kethye P. [UNESP] Endotoxins/toxicity Lasers/diagnostic use Morphine/administration&dosage Photmetry/methods Rabbit Uveitis/chemically induced |
title_short |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
title_full |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
title_fullStr |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
title_full_unstemmed |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
title_sort |
Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis |
author |
Ortencio, Kethye P. [UNESP] |
author_facet |
Ortencio, Kethye P. [UNESP] Renzo, Roberta [UNESP] Sobrinho, Alexandre A.F.B. [UNESP] Kobashigawa, Karina Kamachi [UNESP] Crivelaro, Roberta M. [UNESP] Silva, Germana A. [UNESP] Aldrovani, Marcela [UNESP] Ribeiro, Alexandre Pinto Mineo, Tiago W.P. Laus, José Luiz [UNESP] |
author_role |
author |
author2 |
Renzo, Roberta [UNESP] Sobrinho, Alexandre A.F.B. [UNESP] Kobashigawa, Karina Kamachi [UNESP] Crivelaro, Roberta M. [UNESP] Silva, Germana A. [UNESP] Aldrovani, Marcela [UNESP] Ribeiro, Alexandre Pinto Mineo, Tiago W.P. Laus, José Luiz [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Ortencio, Kethye P. [UNESP] Renzo, Roberta [UNESP] Sobrinho, Alexandre A.F.B. [UNESP] Kobashigawa, Karina Kamachi [UNESP] Crivelaro, Roberta M. [UNESP] Silva, Germana A. [UNESP] Aldrovani, Marcela [UNESP] Ribeiro, Alexandre Pinto Mineo, Tiago W.P. Laus, José Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Endotoxins/toxicity Lasers/diagnostic use Morphine/administration&dosage Photmetry/methods Rabbit Uveitis/chemically induced |
topic |
Endotoxins/toxicity Lasers/diagnostic use Morphine/administration&dosage Photmetry/methods Rabbit Uveitis/chemically induced |
description |
Purpose: To evaluate the effects of 1% morphine instillation on clinical parameters, aqueous humor turbidity, and expression levels of tumor necrosis factor alpha (TNF-a), interleukin-1 beta (IL-1beta), prostaglandin E2 (PGE2), and myeloperoxidase (MPO) in rabbits with endotoxin-induced experimental uveitis. Methods: Twenty four New Zealand white rabbits were divided into four groups (n=6 each): control (CG), morphine (MG), naloxone (NG), and morphine-naloxone (MNG) groups. Under dissociative anesthesia, 0.1 mL of solution containing 0.2 μg of lipopolysaccharide (LPS) endotoxin from the Salmonella typhimurium cell wall was injected in the vitreous chamber. Clinical evaluations (conjunctical hyperemia, chemosis blepharospasm, and ocular discharge) and laser flaremetry were performed before (baseline), and 10 and 20 hours after induction of uveitis. Rabbits were subsequently euthanized and eyes were enucleated to quantify expression levels of TNF-α, IL-1 beta, PGE2, and MPO. Results: No significant differences in clinical parameters and flare values were observed between the study groups. TNF-α and IL-1 beta levels increased significantly in the CG, MG, NG, and MNG groups compared to baseline (P<0.05). Significant differences in PGE2 levels were observed between the MG and NMG groups (P<0.05). A trend toward increased MPO activity was observed in response to uveitis induction; however, this trend did not reach statistical significance (P>0.05). Conclusions: Morphine has no effect on clinical parameters, flare, or expression levels of inflammatory mediators in a rabbit model of uveitis induced by intravitreal injection of LPS. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T17:26:42Z 2018-12-11T17:26:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5935/0004-2749.20150098 Arquivos Brasileiros de Oftalmologia, v. 78, n. 6, p. 371-375, 2015. 1678-2925 0004-2749 http://hdl.handle.net/11449/177703 10.5935/0004-2749.20150098 2-s2.0-84953726710 2-s2.0-84953726710.pdf |
url |
http://dx.doi.org/10.5935/0004-2749.20150098 http://hdl.handle.net/11449/177703 |
identifier_str_mv |
Arquivos Brasileiros de Oftalmologia, v. 78, n. 6, p. 371-375, 2015. 1678-2925 0004-2749 10.5935/0004-2749.20150098 2-s2.0-84953726710 2-s2.0-84953726710.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia 0,518 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
371-375 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964937419751424 |