A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3109/13880209.2015.1107108 http://hdl.handle.net/11449/161878 |
Resumo: | ContextSiolmatra brasiliensis (Cogn.) Baill (Cucurbitaceae) is a climbing plant widely used for the treatment of diabetes mellitus symptoms.Objective This work evaluates the antidiabetic activity of an extract of S. brasiliensis in streptozotocin-diabetic rats and promotes the phytochemical investigation to isolate the major compounds of the same extract.Materials and methods Male Wistar rats were divided into normal (N) and diabetic rats (DC) treated with water; diabetic rats treated with 3U insulin (DI) or with 250 (DSb250) or 500mg/kg (DSb500) of hydroalcoholic extract of the stalks of S. brasiliensis, via oral gavage, for 21 days. Physiological and biochemical parameters classically altered in diabetes were monitored. The triterpenoids were isolated from the ethyl acetate fraction under silica gel column chromatography and Sephadex-LH20 methods and their structures were determined by NMR, HR-ESI-MS and DC analysis.Results When compared with DC, DSb250 rats showed a reduction in the hyperglycemia (DC: 26.460.69 versus DSb250: 19.67 +/- 1.06mmol/L) and glycosuria (DC: 43.02 +/- 3.19 versus DSb250: 28.46 +/- 2.14mmol/24h) and increase in hepatic glycogen (DC: 14.44 +/- 1.26 versus DSb250: 22.08 +/- 4.26mg/g). Three known cucurbitacins were isolated from a hydroalcoholic extract of S. brasiliensis, i.e., cayaponosides A1, B4, D, and a new dammarane saponin 3-O--d-gentiobiosyl-26-O--d-glucopyranosyl-20-hydroxydammar-24-ene. The structures of these compounds were elucidated by spectral data analysis of the natural products and their acetyl derivatives.Discussion and conclusion The known cucurbitacins and/or the new identified saponin may be related with the antidiabetic activity of S. brasiliensis. |
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A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extractAntihyperglycemic effectCucurbitaceaecucurbitacinsdiabetes mellitusContextSiolmatra brasiliensis (Cogn.) Baill (Cucurbitaceae) is a climbing plant widely used for the treatment of diabetes mellitus symptoms.Objective This work evaluates the antidiabetic activity of an extract of S. brasiliensis in streptozotocin-diabetic rats and promotes the phytochemical investigation to isolate the major compounds of the same extract.Materials and methods Male Wistar rats were divided into normal (N) and diabetic rats (DC) treated with water; diabetic rats treated with 3U insulin (DI) or with 250 (DSb250) or 500mg/kg (DSb500) of hydroalcoholic extract of the stalks of S. brasiliensis, via oral gavage, for 21 days. Physiological and biochemical parameters classically altered in diabetes were monitored. The triterpenoids were isolated from the ethyl acetate fraction under silica gel column chromatography and Sephadex-LH20 methods and their structures were determined by NMR, HR-ESI-MS and DC analysis.Results When compared with DC, DSb250 rats showed a reduction in the hyperglycemia (DC: 26.460.69 versus DSb250: 19.67 +/- 1.06mmol/L) and glycosuria (DC: 43.02 +/- 3.19 versus DSb250: 28.46 +/- 2.14mmol/24h) and increase in hepatic glycogen (DC: 14.44 +/- 1.26 versus DSb250: 22.08 +/- 4.26mg/g). Three known cucurbitacins were isolated from a hydroalcoholic extract of S. brasiliensis, i.e., cayaponosides A1, B4, D, and a new dammarane saponin 3-O--d-gentiobiosyl-26-O--d-glucopyranosyl-20-hydroxydammar-24-ene. The structures of these compounds were elucidated by spectral data analysis of the natural products and their acetyl derivatives.Discussion and conclusion The known cucurbitacins and/or the new identified saponin may be related with the antidiabetic activity of S. brasiliensis.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Centro de Pesquisas do Pantanal (CPP)Instituto Nacional de Ciencia e Tecnologia em Areas Umidas (INAU - MT)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Rural Rio De Janeiro, Inst Ciencias Exatas, Dept Quim, Programa Posgrad Quim, Seropedica, RJ, BrazilUniv Fed Mato Grosso, Inst Ciencias Exatas & Terra, Dept Quim, Cuiaba, MT, BrazilUniv Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, Araraquara, SP, BrazilUniv Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, Araraquara, SP, BrazilTaylor & Francis LtdUniv Fed Rural Rio De JaneiroUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Correa dos Santos, Carlos HenriqueBorges, Izabeau PontesSilva, Virginia Claudia daSousa, Paulo Teixeira deKawashita, Nair HondaBaviera, Amanda Martins [UNESP]Carvalho, Mario Geraldo de2018-11-26T17:04:30Z2018-11-26T17:04:30Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1539-1547application/pdfhttp://dx.doi.org/10.3109/13880209.2015.1107108Pharmaceutical Biology. Abingdon: Taylor & Francis Ltd, v. 54, n. 9, p. 1539-1547, 2016.1388-0209http://hdl.handle.net/11449/16187810.3109/13880209.2015.1107108WOS:000382572400006WOS000382572400006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceutical Biology0,563info:eu-repo/semantics/openAccess2024-06-21T15:19:31Zoai:repositorio.unesp.br:11449/161878Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:37:33.922571Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
title |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
spellingShingle |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract Correa dos Santos, Carlos Henrique Antihyperglycemic effect Cucurbitaceae cucurbitacins diabetes mellitus |
title_short |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
title_full |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
title_fullStr |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
title_full_unstemmed |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
title_sort |
A new dammarane saponin and other triterpenoids from Siolmatra brasiliensis and evaluation of the antidiabetic activity of its extract |
author |
Correa dos Santos, Carlos Henrique |
author_facet |
Correa dos Santos, Carlos Henrique Borges, Izabeau Pontes Silva, Virginia Claudia da Sousa, Paulo Teixeira de Kawashita, Nair Honda Baviera, Amanda Martins [UNESP] Carvalho, Mario Geraldo de |
author_role |
author |
author2 |
Borges, Izabeau Pontes Silva, Virginia Claudia da Sousa, Paulo Teixeira de Kawashita, Nair Honda Baviera, Amanda Martins [UNESP] Carvalho, Mario Geraldo de |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Rural Rio De Janeiro Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Correa dos Santos, Carlos Henrique Borges, Izabeau Pontes Silva, Virginia Claudia da Sousa, Paulo Teixeira de Kawashita, Nair Honda Baviera, Amanda Martins [UNESP] Carvalho, Mario Geraldo de |
dc.subject.por.fl_str_mv |
Antihyperglycemic effect Cucurbitaceae cucurbitacins diabetes mellitus |
topic |
Antihyperglycemic effect Cucurbitaceae cucurbitacins diabetes mellitus |
description |
ContextSiolmatra brasiliensis (Cogn.) Baill (Cucurbitaceae) is a climbing plant widely used for the treatment of diabetes mellitus symptoms.Objective This work evaluates the antidiabetic activity of an extract of S. brasiliensis in streptozotocin-diabetic rats and promotes the phytochemical investigation to isolate the major compounds of the same extract.Materials and methods Male Wistar rats were divided into normal (N) and diabetic rats (DC) treated with water; diabetic rats treated with 3U insulin (DI) or with 250 (DSb250) or 500mg/kg (DSb500) of hydroalcoholic extract of the stalks of S. brasiliensis, via oral gavage, for 21 days. Physiological and biochemical parameters classically altered in diabetes were monitored. The triterpenoids were isolated from the ethyl acetate fraction under silica gel column chromatography and Sephadex-LH20 methods and their structures were determined by NMR, HR-ESI-MS and DC analysis.Results When compared with DC, DSb250 rats showed a reduction in the hyperglycemia (DC: 26.460.69 versus DSb250: 19.67 +/- 1.06mmol/L) and glycosuria (DC: 43.02 +/- 3.19 versus DSb250: 28.46 +/- 2.14mmol/24h) and increase in hepatic glycogen (DC: 14.44 +/- 1.26 versus DSb250: 22.08 +/- 4.26mg/g). Three known cucurbitacins were isolated from a hydroalcoholic extract of S. brasiliensis, i.e., cayaponosides A1, B4, D, and a new dammarane saponin 3-O--d-gentiobiosyl-26-O--d-glucopyranosyl-20-hydroxydammar-24-ene. The structures of these compounds were elucidated by spectral data analysis of the natural products and their acetyl derivatives.Discussion and conclusion The known cucurbitacins and/or the new identified saponin may be related with the antidiabetic activity of S. brasiliensis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-11-26T17:04:30Z 2018-11-26T17:04:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3109/13880209.2015.1107108 Pharmaceutical Biology. Abingdon: Taylor & Francis Ltd, v. 54, n. 9, p. 1539-1547, 2016. 1388-0209 http://hdl.handle.net/11449/161878 10.3109/13880209.2015.1107108 WOS:000382572400006 WOS000382572400006.pdf |
url |
http://dx.doi.org/10.3109/13880209.2015.1107108 http://hdl.handle.net/11449/161878 |
identifier_str_mv |
Pharmaceutical Biology. Abingdon: Taylor & Francis Ltd, v. 54, n. 9, p. 1539-1547, 2016. 1388-0209 10.3109/13880209.2015.1107108 WOS:000382572400006 WOS000382572400006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutical Biology 0,563 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1539-1547 application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Ltd |
publisher.none.fl_str_mv |
Taylor & Francis Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129444668243968 |