Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-89132011000300006 http://hdl.handle.net/11449/26576 |
Resumo: | The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which 21 showed differential expression, correlated with desiccation and germination, by increased or decreased expression. After MS/MS sequencing, three protein spots produced spectra that matched to a Magnolia salicifolia legumin precursor. Results suggested an involvement of this protein in the events taking place during the drying and subsequent imbibitions of the dried seeds. |
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Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seedsdesiccation toleranceforest seedleguminproteomicsThe effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which 21 showed differential expression, correlated with desiccation and germination, by increased or decreased expression. After MS/MS sequencing, three protein spots produced spectra that matched to a Magnolia salicifolia legumin precursor. Results suggested an involvement of this protein in the events taking place during the drying and subsequent imbibitions of the dried seeds.Royal Botanic Gardens, Kew, UKMillennium Seed Bank (Kew)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de Lavras (UFLA) Departamento de Ciências FlorestaisUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas de BotucatuRoyal Botanic Gardens Seed Conservation DepartmentUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas de BotucatuBrazilian Archives of Biology and TechnologyUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Royal Botanic Gardens Seed Conservation DepartmentJosé, Anderson CleitonSilva, Edvaldo Aparecido Amaral da [UNESP][Davide, Antonio ClaudioToorop, Peter2014-05-20T15:07:34Z2014-05-20T15:07:34Z2011-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article465-476application/pdfhttp://dx.doi.org/10.1590/S1516-89132011000300006Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 3, p. 465-476, 2011.1516-8913http://hdl.handle.net/11449/2657610.1590/S1516-89132011000300006S1516-89132011000300006WOS:000291849900006S1516-89132011000300006.pdf99726437744913010000-0001-6454-1488SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technology0.6760,281info:eu-repo/semantics/openAccess2024-04-30T15:56:02Zoai:repositorio.unesp.br:11449/26576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:34:40.890243Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
title |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
spellingShingle |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds José, Anderson Cleiton desiccation tolerance forest seed legumin proteomics |
title_short |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
title_full |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
title_fullStr |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
title_full_unstemmed |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
title_sort |
Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds |
author |
José, Anderson Cleiton |
author_facet |
José, Anderson Cleiton Silva, Edvaldo Aparecido Amaral da [UNESP][ Davide, Antonio Claudio Toorop, Peter |
author_role |
author |
author2 |
Silva, Edvaldo Aparecido Amaral da [UNESP][ Davide, Antonio Claudio Toorop, Peter |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras (UFLA) Universidade Estadual Paulista (Unesp) Royal Botanic Gardens Seed Conservation Department |
dc.contributor.author.fl_str_mv |
José, Anderson Cleiton Silva, Edvaldo Aparecido Amaral da [UNESP][ Davide, Antonio Claudio Toorop, Peter |
dc.subject.por.fl_str_mv |
desiccation tolerance forest seed legumin proteomics |
topic |
desiccation tolerance forest seed legumin proteomics |
description |
The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which 21 showed differential expression, correlated with desiccation and germination, by increased or decreased expression. After MS/MS sequencing, three protein spots produced spectra that matched to a Magnolia salicifolia legumin precursor. Results suggested an involvement of this protein in the events taking place during the drying and subsequent imbibitions of the dried seeds. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 2014-05-20T15:07:34Z 2014-05-20T15:07:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-89132011000300006 Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 3, p. 465-476, 2011. 1516-8913 http://hdl.handle.net/11449/26576 10.1590/S1516-89132011000300006 S1516-89132011000300006 WOS:000291849900006 S1516-89132011000300006.pdf 9972643774491301 0000-0001-6454-1488 |
url |
http://dx.doi.org/10.1590/S1516-89132011000300006 http://hdl.handle.net/11449/26576 |
identifier_str_mv |
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 3, p. 465-476, 2011. 1516-8913 10.1590/S1516-89132011000300006 S1516-89132011000300006 WOS:000291849900006 S1516-89132011000300006.pdf 9972643774491301 0000-0001-6454-1488 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Archives of Biology and Technology 0.676 0,281 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
465-476 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Archives of Biology and Technology |
publisher.none.fl_str_mv |
Brazilian Archives of Biology and Technology |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128674043527168 |