Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Nancy Bueno [UNESP]
Data de Publicação: 2014
Outros Autores: Cestari, Silvia Helena [UNESP], Conde, Sandro Jose [UNESP], Melo Luvizotto, Renata Azevedo [UNESP], De Sibio, Maria Teresa [UNESP], Perone, Denise [UNESP], Hirata Katayama, Maria Lucia, Carraro, Dirce Maria, Brentani, Helena Paula, Brentani, Maria Mitzi, Nogueira, Célia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2014/969404
http://hdl.handle.net/11449/112185
Resumo: It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T-3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E-2), and suppress genes (TGF-beta) normally inhibited by E-2. Since T-3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E-2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E-2 and T-3. Several genes were modulated by both E-2 and T-3 in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E-2 and T-3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E-2 and T-3.
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spelling Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell LineIt has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T-3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E-2), and suppress genes (TGF-beta) normally inhibited by E-2. Since T-3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E-2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E-2 and T-3. Several genes were modulated by both E-2 and T-3 in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E-2 and T-3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E-2 and T-3.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, BrazilUniv Sao Paulo, Sch Med, Dept Radiol, BR-01246903 Sao Paulo, BrazilAC Camargo Hosp, Res Ctr, BR-01509900 Sao Paulo, BrazilUniv Sao Paulo State, Dept Clin Med, BR-18618000 Botucatu, SP, BrazilUniv Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, BrazilFAPESP: 02/09798-0Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)AC Camargo HospFigueiredo, Nancy Bueno [UNESP]Cestari, Silvia Helena [UNESP]Conde, Sandro Jose [UNESP]Melo Luvizotto, Renata Azevedo [UNESP]De Sibio, Maria Teresa [UNESP]Perone, Denise [UNESP]Hirata Katayama, Maria LuciaCarraro, Dirce MariaBrentani, Helena PaulaBrentani, Maria MitziNogueira, Célia Regina [UNESP]2014-12-03T13:10:29Z2014-12-03T13:10:29Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1155/2014/969404Scientific World Journal. New York: Hindawi Publishing Corporation, 7 p., 2014.1537-744Xhttp://hdl.handle.net/11449/11218510.1155/2014/969404WOS:000330657200001WOS000330657200001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2024-08-14T17:22:59Zoai:repositorio.unesp.br:11449/112185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
title Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
spellingShingle Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
Figueiredo, Nancy Bueno [UNESP]
title_short Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
title_full Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
title_fullStr Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
title_full_unstemmed Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
title_sort Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
author Figueiredo, Nancy Bueno [UNESP]
author_facet Figueiredo, Nancy Bueno [UNESP]
Cestari, Silvia Helena [UNESP]
Conde, Sandro Jose [UNESP]
Melo Luvizotto, Renata Azevedo [UNESP]
De Sibio, Maria Teresa [UNESP]
Perone, Denise [UNESP]
Hirata Katayama, Maria Lucia
Carraro, Dirce Maria
Brentani, Helena Paula
Brentani, Maria Mitzi
Nogueira, Célia Regina [UNESP]
author_role author
author2 Cestari, Silvia Helena [UNESP]
Conde, Sandro Jose [UNESP]
Melo Luvizotto, Renata Azevedo [UNESP]
De Sibio, Maria Teresa [UNESP]
Perone, Denise [UNESP]
Hirata Katayama, Maria Lucia
Carraro, Dirce Maria
Brentani, Helena Paula
Brentani, Maria Mitzi
Nogueira, Célia Regina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
AC Camargo Hosp
dc.contributor.author.fl_str_mv Figueiredo, Nancy Bueno [UNESP]
Cestari, Silvia Helena [UNESP]
Conde, Sandro Jose [UNESP]
Melo Luvizotto, Renata Azevedo [UNESP]
De Sibio, Maria Teresa [UNESP]
Perone, Denise [UNESP]
Hirata Katayama, Maria Lucia
Carraro, Dirce Maria
Brentani, Helena Paula
Brentani, Maria Mitzi
Nogueira, Célia Regina [UNESP]
description It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T-3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E-2), and suppress genes (TGF-beta) normally inhibited by E-2. Since T-3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E-2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E-2 and T-3. Several genes were modulated by both E-2 and T-3 in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E-2 and T-3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E-2 and T-3.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:10:29Z
2014-12-03T13:10:29Z
2014-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2014/969404
Scientific World Journal. New York: Hindawi Publishing Corporation, 7 p., 2014.
1537-744X
http://hdl.handle.net/11449/112185
10.1155/2014/969404
WOS:000330657200001
WOS000330657200001.pdf
url http://dx.doi.org/10.1155/2014/969404
http://hdl.handle.net/11449/112185
identifier_str_mv Scientific World Journal. New York: Hindawi Publishing Corporation, 7 p., 2014.
1537-744X
10.1155/2014/969404
WOS:000330657200001
WOS000330657200001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific World Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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