Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System

Detalhes bibliográficos
Autor(a) principal: Zimmer, R.
Data de Publicação: 2022
Outros Autores: Leite, M. L. [UNESP], Costa, C. A. de Souza [UNESP], Hebling, J. [UNESP], Anovazzi, G. [UNESP], Klein, C. A., Hosaka, K., Reston, E. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2341/20-038-L
http://hdl.handle.net/11449/241048
Resumo: OBJECTIVES: To evaluate the influence of heat application on the degree of conversion (DC) of the 3M Single Bond Universal Adhesive System, as well as its transdentinal cytotoxicity and microtensile bond strength to dentin. METHODS: Experimental groups were established according to the time and temperature of the air jet: G1: 5 seconds-25°C; G2: 10 seconds-25°C; G3: 20 seconds-25°C; G4: 5 seconds-50°C; G5: 10 seconds-50°C; G6: 20 seconds-50°C. In control group (G7), no treatment was performed. The DC was assessed using the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) technique. For the transdentinal cytotoxicity test, dentin discs fitted in artificial pulp chambers (APC) received the application of the adhesive system and the air jets. For the microtensile bond strength, healthy molars were restored and submitted to the microtensile test after 24 hours and 6 months, respectively. RESULTS: Significant reduction in viability of Mouse Dental Papilla Cell-23 (MDPC-23), which exhibited morphological changes, was observed in all experimental groups compared to control (p<0.05). Although all tested protocols resulted in transdentinal diffusion of 2-hydroxyethyl methacrylate (HEMA), the group G6 presented the highest degree of monomeric conversion and the lowest cytotoxic effect, with higher dentin bond strength values in comparison to group G1 (p<0.05). CONCLUSIONS: Applying an air blast at 50°C for 20 seconds increases the DC and microtensile bond strength of the 3M Single Bond Universal Adhesive System to dentin, as well as reduces the transdentinal cytotoxicity of the material to pulp cells.
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spelling Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive SystemOBJECTIVES: To evaluate the influence of heat application on the degree of conversion (DC) of the 3M Single Bond Universal Adhesive System, as well as its transdentinal cytotoxicity and microtensile bond strength to dentin. METHODS: Experimental groups were established according to the time and temperature of the air jet: G1: 5 seconds-25°C; G2: 10 seconds-25°C; G3: 20 seconds-25°C; G4: 5 seconds-50°C; G5: 10 seconds-50°C; G6: 20 seconds-50°C. In control group (G7), no treatment was performed. The DC was assessed using the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) technique. For the transdentinal cytotoxicity test, dentin discs fitted in artificial pulp chambers (APC) received the application of the adhesive system and the air jets. For the microtensile bond strength, healthy molars were restored and submitted to the microtensile test after 24 hours and 6 months, respectively. RESULTS: Significant reduction in viability of Mouse Dental Papilla Cell-23 (MDPC-23), which exhibited morphological changes, was observed in all experimental groups compared to control (p<0.05). Although all tested protocols resulted in transdentinal diffusion of 2-hydroxyethyl methacrylate (HEMA), the group G6 presented the highest degree of monomeric conversion and the lowest cytotoxic effect, with higher dentin bond strength values in comparison to group G1 (p<0.05). CONCLUSIONS: Applying an air blast at 50°C for 20 seconds increases the DC and microtensile bond strength of the 3M Single Bond Universal Adhesive System to dentin, as well as reduces the transdentinal cytotoxicity of the material to pulp cells.DDS MS Department of Operative Dentistry School of Dentistry Lutheran University of Brazil (Ulbra)DDS MS Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)Carlos Alberto de Souza Costa DDS MS Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)DDS MS Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Celso Afonso Klein-Junior DDS MS Department of Operative Dentistry School of Dentistry Lutheran University of Brazil (Ulbra)DDS MS Department of Cariology and Operative Dentistry Graduate School of Medical and Dental Sciences Tokyo Medical and Dental UniversityDDS MSD Department of Operative Dentistry School of Dentistry Lutheran University of Brazil (Ulbra)DDS MS Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)Carlos Alberto de Souza Costa DDS MS Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)DDS MS Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Lutheran University of Brazil (Ulbra)Universidade Estadual Paulista (UNESP)Tokyo Medical and Dental UniversityZimmer, R.Leite, M. L. [UNESP]Costa, C. A. de Souza [UNESP]Hebling, J. [UNESP]Anovazzi, G. [UNESP]Klein, C. A.Hosaka, K.Reston, E. D.2023-03-01T20:44:38Z2023-03-01T20:44:38Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article87-96http://dx.doi.org/10.2341/20-038-LOperative dentistry, v. 47, n. 1, p. 87-96, 2022.1559-2863http://hdl.handle.net/11449/24104810.2341/20-038-L2-s2.0-85130767041Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOperative dentistryinfo:eu-repo/semantics/openAccess2023-03-01T20:44:39Zoai:repositorio.unesp.br:11449/241048Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:44:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
title Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
spellingShingle Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
Zimmer, R.
title_short Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
title_full Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
title_fullStr Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
title_full_unstemmed Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
title_sort Effect of Time and Temperature of Air Jet on the Mechanical and Biological Behavior of a Universal Adhesive System
author Zimmer, R.
author_facet Zimmer, R.
Leite, M. L. [UNESP]
Costa, C. A. de Souza [UNESP]
Hebling, J. [UNESP]
Anovazzi, G. [UNESP]
Klein, C. A.
Hosaka, K.
Reston, E. D.
author_role author
author2 Leite, M. L. [UNESP]
Costa, C. A. de Souza [UNESP]
Hebling, J. [UNESP]
Anovazzi, G. [UNESP]
Klein, C. A.
Hosaka, K.
Reston, E. D.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Lutheran University of Brazil (Ulbra)
Universidade Estadual Paulista (UNESP)
Tokyo Medical and Dental University
dc.contributor.author.fl_str_mv Zimmer, R.
Leite, M. L. [UNESP]
Costa, C. A. de Souza [UNESP]
Hebling, J. [UNESP]
Anovazzi, G. [UNESP]
Klein, C. A.
Hosaka, K.
Reston, E. D.
description OBJECTIVES: To evaluate the influence of heat application on the degree of conversion (DC) of the 3M Single Bond Universal Adhesive System, as well as its transdentinal cytotoxicity and microtensile bond strength to dentin. METHODS: Experimental groups were established according to the time and temperature of the air jet: G1: 5 seconds-25°C; G2: 10 seconds-25°C; G3: 20 seconds-25°C; G4: 5 seconds-50°C; G5: 10 seconds-50°C; G6: 20 seconds-50°C. In control group (G7), no treatment was performed. The DC was assessed using the Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) technique. For the transdentinal cytotoxicity test, dentin discs fitted in artificial pulp chambers (APC) received the application of the adhesive system and the air jets. For the microtensile bond strength, healthy molars were restored and submitted to the microtensile test after 24 hours and 6 months, respectively. RESULTS: Significant reduction in viability of Mouse Dental Papilla Cell-23 (MDPC-23), which exhibited morphological changes, was observed in all experimental groups compared to control (p<0.05). Although all tested protocols resulted in transdentinal diffusion of 2-hydroxyethyl methacrylate (HEMA), the group G6 presented the highest degree of monomeric conversion and the lowest cytotoxic effect, with higher dentin bond strength values in comparison to group G1 (p<0.05). CONCLUSIONS: Applying an air blast at 50°C for 20 seconds increases the DC and microtensile bond strength of the 3M Single Bond Universal Adhesive System to dentin, as well as reduces the transdentinal cytotoxicity of the material to pulp cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T20:44:38Z
2023-03-01T20:44:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2341/20-038-L
Operative dentistry, v. 47, n. 1, p. 87-96, 2022.
1559-2863
http://hdl.handle.net/11449/241048
10.2341/20-038-L
2-s2.0-85130767041
url http://dx.doi.org/10.2341/20-038-L
http://hdl.handle.net/11449/241048
identifier_str_mv Operative dentistry, v. 47, n. 1, p. 87-96, 2022.
1559-2863
10.2341/20-038-L
2-s2.0-85130767041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Operative dentistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 87-96
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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