Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)

Detalhes bibliográficos
Autor(a) principal: Biller-takahashi, J.d. [UNESP]
Data de Publicação: 2013
Outros Autores: Takahashi, L.s. [UNESP], Pilarski, F., Sebastião, F.a., Urbinati, E.c. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1590/S0102-09352013000600023
Texto Completo: http://dx.doi.org/10.1590/S0102-09352013000600023
http://hdl.handle.net/11449/109512
Resumo: The immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.
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spelling Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)Atividade bactericida do soro como indicador de imunidade inata de pacu Piaractus mesopotamicus (Holmberg, 1887)imunologia inata de peixesmetodologiaimunidade humoralfish innate immunologymethodologyhumoral immunityThe immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.O sistema imune de peixes teleósteos tem mecanismos responsáveis pela defesa contra bactérias e atua através de proteínas presentes em diversos tecidos. A ação destas proteínas pode ser avaliada pela atividade bactericida do soro, sendo esta uma importante ferramenta para analisar o sistema imune. O pacu, Piaractus mesopotamicus, é um peixe nativo muito importante para aquicultura nacional, entretanto há pouco conhecimento sobre o funcionamento de seu sistema imune. Assim foi realizado experimento para padronizar e avaliar a eficiência do ensaio de atividade bactericida. Resumidamente, peixes foram desafiados por Aeromonas hydrophila e amostradas uma semana após o desafio. A infecção bacteriana promoveu um aumento na concentração de proteínas protetoras, resultando em diminuição dos valores de unidades formadoras de colônias ou expressos também como aumento da atividade bactericida do soro. O protocolo se mostrou confiável, sendo apropriado para determinar a atividade bactericida do soro de pacu nas condições experimentais.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual PaulistaUniversidade Estadual Paulista Faculdade de ZootecniaUniversidade Estadual PaulistaUniversidade Estadual Paulista Faculdade de ZootecniaUniversidade Federal de Minas Gerais (UFMG), Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Biller-takahashi, J.d. [UNESP]Takahashi, L.s. [UNESP]Pilarski, F.Sebastião, F.a.Urbinati, E.c. [UNESP]2014-09-30T18:18:25Z2014-09-30T18:18:25Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1745-1751application/pdfhttp://dx.doi.org/10.1590/S0102-09352013000600023Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 6, p. 1745-1751, 2013.0102-0935http://hdl.handle.net/11449/10951210.1590/S0102-09352013000600023S0102-09352013000600023WOS:000329450600023S0102-09352013000600023.pdf0089991497257336SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-06-06T18:41:55Zoai:repositorio.unesp.br:11449/109512Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:12:13.338182Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
Atividade bactericida do soro como indicador de imunidade inata de pacu Piaractus mesopotamicus (Holmberg, 1887)
title Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
spellingShingle Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
Biller-takahashi, J.d. [UNESP]
imunologia inata de peixes
metodologia
imunidade humoral
fish innate immunology
methodology
humoral immunity
Biller-takahashi, J.d. [UNESP]
imunologia inata de peixes
metodologia
imunidade humoral
fish innate immunology
methodology
humoral immunity
title_short Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
title_full Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
title_fullStr Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
title_full_unstemmed Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
title_sort Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)
author Biller-takahashi, J.d. [UNESP]
author_facet Biller-takahashi, J.d. [UNESP]
Biller-takahashi, J.d. [UNESP]
Takahashi, L.s. [UNESP]
Pilarski, F.
Sebastião, F.a.
Urbinati, E.c. [UNESP]
Takahashi, L.s. [UNESP]
Pilarski, F.
Sebastião, F.a.
Urbinati, E.c. [UNESP]
author_role author
author2 Takahashi, L.s. [UNESP]
Pilarski, F.
Sebastião, F.a.
Urbinati, E.c. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Biller-takahashi, J.d. [UNESP]
Takahashi, L.s. [UNESP]
Pilarski, F.
Sebastião, F.a.
Urbinati, E.c. [UNESP]
dc.subject.por.fl_str_mv imunologia inata de peixes
metodologia
imunidade humoral
fish innate immunology
methodology
humoral immunity
topic imunologia inata de peixes
metodologia
imunidade humoral
fish innate immunology
methodology
humoral immunity
description The immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2014-09-30T18:18:25Z
2014-09-30T18:18:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-09352013000600023
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 6, p. 1745-1751, 2013.
0102-0935
http://hdl.handle.net/11449/109512
10.1590/S0102-09352013000600023
S0102-09352013000600023
WOS:000329450600023
S0102-09352013000600023.pdf
0089991497257336
url http://dx.doi.org/10.1590/S0102-09352013000600023
http://hdl.handle.net/11449/109512
identifier_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 6, p. 1745-1751, 2013.
0102-0935
10.1590/S0102-09352013000600023
S0102-09352013000600023
WOS:000329450600023
S0102-09352013000600023.pdf
0089991497257336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
0.286
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1745-1751
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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dc.identifier.doi.none.fl_str_mv 10.1590/S0102-09352013000600023

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