Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista

Detalhes bibliográficos
Autor(a) principal: Pedro, Heloisa da Silveira Paro [UNESP]
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/127890
Resumo: Tuberculosis is considered a high priority public health problem. There is increasing evidence that the variation in Mycobacterium tuberculosis strains play an important role in the outcome of infections by tuberculosis. Apart from describing sociodemographic, clinical, epidemiological and bacteriological aspects of tuberculosis (TB) and correlating the distribution of resistance to antituberculosis drugs, one objective of this study was to evaluate the diversity of the genotypes of M. tuberculosis isolates using the Spoligotyping (spacer oligonucleotide typing) and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units variable-number tandem repeats) techniques. With respect to drug resistance, cultures of M. tuberculosis and susceptibility tests showed that strains from people previously submitted to TB treatment or infected with HIV have more resistance to drugs compared to those without these characteristics (p <0.05 ). Resistant cases are mostly pulmonary M. tuberculosis, and have positive smear test results. Molecular analysis showed that of the 377 samples, 14 MIRU-VNTR loci had complete profiles; the 10, 16, 23, 26, 27, 31, 39 and 40 loci had high discriminatory power (0.6) the 2, ETRB and Mtub21 loci were moderately discriminatory (0.3) and 4, 20 and 24 loci had low discriminatory power (0.3). Ninety-two different patterns were observed in the 250 M. tuberculosis isolates analyzed by spoligotyping and different families were identified as Haarlem (H), Latin American and Mediterranean (LAM), T Family, undesignated (U), S lineage and X Family. Significant genetic variability was observed in M. tuberculosis isolates circulating in the northwestern region of Sao Paulo State when analyzed by spoligotyping and MIRU-VNTR
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spelling Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulistaGenéticaEpidemiologia molecularTuberculoseMycobacterium tuberculosisTuberculosis is considered a high priority public health problem. There is increasing evidence that the variation in Mycobacterium tuberculosis strains play an important role in the outcome of infections by tuberculosis. Apart from describing sociodemographic, clinical, epidemiological and bacteriological aspects of tuberculosis (TB) and correlating the distribution of resistance to antituberculosis drugs, one objective of this study was to evaluate the diversity of the genotypes of M. tuberculosis isolates using the Spoligotyping (spacer oligonucleotide typing) and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units variable-number tandem repeats) techniques. With respect to drug resistance, cultures of M. tuberculosis and susceptibility tests showed that strains from people previously submitted to TB treatment or infected with HIV have more resistance to drugs compared to those without these characteristics (p <0.05 ). Resistant cases are mostly pulmonary M. tuberculosis, and have positive smear test results. Molecular analysis showed that of the 377 samples, 14 MIRU-VNTR loci had complete profiles; the 10, 16, 23, 26, 27, 31, 39 and 40 loci had high discriminatory power (0.6) the 2, ETRB and Mtub21 loci were moderately discriminatory (0.3) and 4, 20 and 24 loci had low discriminatory power (0.3). Ninety-two different patterns were observed in the 250 M. tuberculosis isolates analyzed by spoligotyping and different families were identified as Haarlem (H), Latin American and Mediterranean (LAM), T Family, undesignated (U), S lineage and X Family. Significant genetic variability was observed in M. tuberculosis isolates circulating in the northwestern region of Sao Paulo State when analyzed by spoligotyping and MIRU-VNTRA tuberculose é considerada um problema prioritário de saúde pública. Há cada vez mais evidências de que, a variação das cepas de M. tuberculosis tem um papel importante no resultado da infecção por tuberculose. No presente estudo, além de descrever os aspectos sociodemográficos, clínico-epidemiológicos e bacteriológicos da tuberculose e relacionar com a distribuição de resistência aos fármacos antituberculose, também objetivou-se avaliar a diversidade dos genótipos de isolados de Mycobacterium tuberculosis utilizando as técnicas de Spoligotipyng (Spacer Oligonucleotide Typing) e MIRU-VNTR (Mycobacterial Interspersed Repetitive Units-variable-number tandem repeats). Com relação à resistência aos fármacos, as culturas de MT e os testes de sensibilidade mostram que cepas de pessoas que fizeram tratamento de TB anterior ou infectados pelo HIV apresentaram mais resistência os fármacos quando comparados aos que não apresentavam essas características (p<0,05). Os casos resistentes, em sua maioria, são de origem pulmonar e apresentam baciloscopia positiva. A análise molecular mostrou que das 377 amostras com perfis completos para 14 loci MIRU-VNTR, os loci 10, 16, 23, 26, 27, 31, 39 e 40 tiveram um alto poder discriminatório (0.6), os loci 2, ETRB e Mtub21 foram moderadamente discriminatórios (0.3) e loci 4, 20 e 24, baixo poder discriminatório (0.3). Dos 250 isolados de M. tuberculosis analisados por spoligotyping, 92 diferentes padrões foram observados e diferentes famílias foram identificadas: Haarlem (H), Latin American and Mediterraneam (LAM), T Family, undesignated (U), S lineage e X Family. Significativa variabilidade genética foi observada nos isolados de M. tuberculosis circulantes na região Noroeste Paulista quando analisados por spoligotyping e MIRU-VNTRUniversidade Estadual Paulista (Unesp)Castiglioni, Lilian [UNESP]Universidade Estadual Paulista (Unesp)Pedro, Heloisa da Silveira Paro [UNESP]2015-09-17T15:26:18Z2015-09-17T15:26:18Z2015-05-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis141 f. : il. tabs. color.application/pdfPEDRO, Heloisa da Silveira Paro. Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista. 2015. 141 f. Tese (doutorado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2015.http://hdl.handle.net/11449/127890000844405000844405.pdf33004153023P5Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-14T06:09:09Zoai:repositorio.unesp.br:11449/127890Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:54:43.472967Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
title Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
spellingShingle Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
Pedro, Heloisa da Silveira Paro [UNESP]
Genética
Epidemiologia molecular
Tuberculose
Mycobacterium tuberculosis
title_short Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
title_full Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
title_fullStr Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
title_full_unstemmed Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
title_sort Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista
author Pedro, Heloisa da Silveira Paro [UNESP]
author_facet Pedro, Heloisa da Silveira Paro [UNESP]
author_role author
dc.contributor.none.fl_str_mv Castiglioni, Lilian [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pedro, Heloisa da Silveira Paro [UNESP]
dc.subject.por.fl_str_mv Genética
Epidemiologia molecular
Tuberculose
Mycobacterium tuberculosis
topic Genética
Epidemiologia molecular
Tuberculose
Mycobacterium tuberculosis
description Tuberculosis is considered a high priority public health problem. There is increasing evidence that the variation in Mycobacterium tuberculosis strains play an important role in the outcome of infections by tuberculosis. Apart from describing sociodemographic, clinical, epidemiological and bacteriological aspects of tuberculosis (TB) and correlating the distribution of resistance to antituberculosis drugs, one objective of this study was to evaluate the diversity of the genotypes of M. tuberculosis isolates using the Spoligotyping (spacer oligonucleotide typing) and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units variable-number tandem repeats) techniques. With respect to drug resistance, cultures of M. tuberculosis and susceptibility tests showed that strains from people previously submitted to TB treatment or infected with HIV have more resistance to drugs compared to those without these characteristics (p <0.05 ). Resistant cases are mostly pulmonary M. tuberculosis, and have positive smear test results. Molecular analysis showed that of the 377 samples, 14 MIRU-VNTR loci had complete profiles; the 10, 16, 23, 26, 27, 31, 39 and 40 loci had high discriminatory power (0.6) the 2, ETRB and Mtub21 loci were moderately discriminatory (0.3) and 4, 20 and 24 loci had low discriminatory power (0.3). Ninety-two different patterns were observed in the 250 M. tuberculosis isolates analyzed by spoligotyping and different families were identified as Haarlem (H), Latin American and Mediterranean (LAM), T Family, undesignated (U), S lineage and X Family. Significant genetic variability was observed in M. tuberculosis isolates circulating in the northwestern region of Sao Paulo State when analyzed by spoligotyping and MIRU-VNTR
publishDate 2015
dc.date.none.fl_str_mv 2015-09-17T15:26:18Z
2015-09-17T15:26:18Z
2015-05-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PEDRO, Heloisa da Silveira Paro. Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista. 2015. 141 f. Tese (doutorado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2015.
http://hdl.handle.net/11449/127890
000844405
000844405.pdf
33004153023P5
identifier_str_mv PEDRO, Heloisa da Silveira Paro. Diversidade clínica, epidemiológica e Genética do Mycobacterium tuberculosis na região Noroeste paulista. 2015. 141 f. Tese (doutorado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas, 2015.
000844405
000844405.pdf
33004153023P5
url http://hdl.handle.net/11449/127890
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 141 f. : il. tabs. color.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128435740999680

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