Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0134607 http://hdl.handle.net/11449/131068 |
Resumo: | The rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which were shared between groups and 89 that were private in different groups of accessions. In a population structure and principal component analysis, the level of clustering reflected a primary division into the following two subgroups: cluster 1, which consisted of varieties from the advanced breeding germplasm that originated from the Wickham and Mato Grosso accessions; and cluster 2, which consisted of the wild germplasm from the Acre, Amazonas, Pará and Rondônia populations and Hevea spp. The analyses revealed a high frequency of gene flow between the groups, with the genetic differentiation coefficient (GST) estimated to be 0.018. Additionally, no distinct separation among the H. brasiliensis accessions and the other species from Amazonas was observed. A core collection of 99 accessions was identified that captured the maximum genetic diversity. Rubber tree breeders can effectively utilize this core collection for cultivar improvement. Furthermore, such a core collection could provide resources for forming an association panel to evaluate traits with agronomic and commercial importance. Our study generated a molecular database that should facilitate the management of the Hevea germplasm and its use for subsequent genetic and genomic breeding. |
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Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collectionThe rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which were shared between groups and 89 that were private in different groups of accessions. In a population structure and principal component analysis, the level of clustering reflected a primary division into the following two subgroups: cluster 1, which consisted of varieties from the advanced breeding germplasm that originated from the Wickham and Mato Grosso accessions; and cluster 2, which consisted of the wild germplasm from the Acre, Amazonas, Pará and Rondônia populations and Hevea spp. The analyses revealed a high frequency of gene flow between the groups, with the genetic differentiation coefficient (GST) estimated to be 0.018. Additionally, no distinct separation among the H. brasiliensis accessions and the other species from Amazonas was observed. A core collection of 99 accessions was identified that captured the maximum genetic diversity. Rubber tree breeders can effectively utilize this core collection for cultivar improvement. Furthermore, such a core collection could provide resources for forming an association panel to evaluate traits with agronomic and commercial importance. Our study generated a molecular database that should facilitate the management of the Hevea germplasm and its use for subsequent genetic and genomic breeding.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento do Pessoal de Nível Superior (CAPES)Molecular Biology and Genetic Engineering Center (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil.Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) UMR AGAP, Montpellier, Hérault, France.Molecular Biology and Genetic Engineering Center (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil; Laboratory of Applied Molecular Genetics, Department of Exact and Natural Sciences, State University of Southwest Bahia (UESB), Itapetinga, BA, Brazil.Molecular Biology and Genetic Engineering Center (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil; Agência Paulista de Tecnologia dos Agronegócios (APTA), Pólo Regional Noroeste Paulista, Votuporanga, SP, Brazil.Agência Paulista de Tecnologia dos Agronegócios (APTA), Pólo Regional Noroeste Paulista, Votuporanga, SP, Brazil.Rubber Research Advanced Center (CAPSA), Agronomical Institute (IAC), Votuporanga, SP, Brazil.Embrapa Cerrados (EMBRAPA), Planaltina, DF, Brasil.Departamento de Fitotecnia, Faculdade de Engenharia de Ilha Solteira (UNESP)-Universidade Estadual Paulista, Ilha Solteira, SP, Brazil.Molecular Biology and Genetic Engineering Center (CBMEG), University of Campinas (UNICAMP), Campinas, SP, Brazil; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP) UNICAMP, Campinas, SP, Brazil.Departamento de Fitotecnia, Faculdade de Engenharia de Ilha Solteira (UNESP)-Universidade Estadual Paulista, Ilha Solteira, SP, Brazil.FAPESP: 2007/50392-1FAPESP: 2012/50491-8FAPESP: 2011/50188-0FAPESP: 2009/52975-0FAPESP: 2012/05473-1CNPq: 478701/2012-8CNPq: 402954/2012-2Public Library ScienceUniversidade Estadual de Campinas (UNICAMP)Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)Agência Paulista de Tecnologia dos Agronegócios (APTA)Agronomical Institute (IAC)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Souza, Livia Moura deLe Guen, VincentCerqueira-Silva, Carlos Bernardo MorenoSilva, Carla CristinaMantello, Camila CamposConson, Andre Ricardo OliveiraVianna, João Paulo GomesZucchi, Maria ImaculadaScaloppi Junior, Erivaldo JoséFialho, Josefino de Freitasde Moraes, Mario Luis Teixeira [UNESP]Gonçalves, Paulo de SouzaSouza, Anete Pereira de2015-12-07T15:31:19Z2015-12-07T15:31:19Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0134607Plos One, v. 10, n. 7, 2015.1932-6203http://hdl.handle.net/11449/13106810.1371/journal.pone.0134607PMC4520663.pdf980342667222180226225861PMC4520663PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2024-07-05T19:08:46Zoai:repositorio.unesp.br:11449/131068Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:21:48.023799Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
title |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
spellingShingle |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection Souza, Livia Moura de |
title_short |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
title_full |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
title_fullStr |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
title_full_unstemmed |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
title_sort |
Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-genotype core collection |
author |
Souza, Livia Moura de |
author_facet |
Souza, Livia Moura de Le Guen, Vincent Cerqueira-Silva, Carlos Bernardo Moreno Silva, Carla Cristina Mantello, Camila Campos Conson, Andre Ricardo Oliveira Vianna, João Paulo Gomes Zucchi, Maria Imaculada Scaloppi Junior, Erivaldo José Fialho, Josefino de Freitas de Moraes, Mario Luis Teixeira [UNESP] Gonçalves, Paulo de Souza Souza, Anete Pereira de |
author_role |
author |
author2 |
Le Guen, Vincent Cerqueira-Silva, Carlos Bernardo Moreno Silva, Carla Cristina Mantello, Camila Campos Conson, Andre Ricardo Oliveira Vianna, João Paulo Gomes Zucchi, Maria Imaculada Scaloppi Junior, Erivaldo José Fialho, Josefino de Freitas de Moraes, Mario Luis Teixeira [UNESP] Gonçalves, Paulo de Souza Souza, Anete Pereira de |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) Agência Paulista de Tecnologia dos Agronegócios (APTA) Agronomical Institute (IAC) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Souza, Livia Moura de Le Guen, Vincent Cerqueira-Silva, Carlos Bernardo Moreno Silva, Carla Cristina Mantello, Camila Campos Conson, Andre Ricardo Oliveira Vianna, João Paulo Gomes Zucchi, Maria Imaculada Scaloppi Junior, Erivaldo José Fialho, Josefino de Freitas de Moraes, Mario Luis Teixeira [UNESP] Gonçalves, Paulo de Souza Souza, Anete Pereira de |
description |
The rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which were shared between groups and 89 that were private in different groups of accessions. In a population structure and principal component analysis, the level of clustering reflected a primary division into the following two subgroups: cluster 1, which consisted of varieties from the advanced breeding germplasm that originated from the Wickham and Mato Grosso accessions; and cluster 2, which consisted of the wild germplasm from the Acre, Amazonas, Pará and Rondônia populations and Hevea spp. The analyses revealed a high frequency of gene flow between the groups, with the genetic differentiation coefficient (GST) estimated to be 0.018. Additionally, no distinct separation among the H. brasiliensis accessions and the other species from Amazonas was observed. A core collection of 99 accessions was identified that captured the maximum genetic diversity. Rubber tree breeders can effectively utilize this core collection for cultivar improvement. Furthermore, such a core collection could provide resources for forming an association panel to evaluate traits with agronomic and commercial importance. Our study generated a molecular database that should facilitate the management of the Hevea germplasm and its use for subsequent genetic and genomic breeding. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:31:19Z 2015-12-07T15:31:19Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0134607 Plos One, v. 10, n. 7, 2015. 1932-6203 http://hdl.handle.net/11449/131068 10.1371/journal.pone.0134607 PMC4520663.pdf 9803426672221802 26225861 PMC4520663 |
url |
http://dx.doi.org/10.1371/journal.pone.0134607 http://hdl.handle.net/11449/131068 |
identifier_str_mv |
Plos One, v. 10, n. 7, 2015. 1932-6203 10.1371/journal.pone.0134607 PMC4520663.pdf 9803426672221802 26225861 PMC4520663 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129420476547072 |