Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242011000100005 http://hdl.handle.net/11449/226243 |
Resumo: | The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure. |
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Effectiveness of 2% peracetic acid for the disinfection of gutta-percha conesDisinfectionEndodonticsMicrobiologyThe aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.Department of Biosciences and Oral Diagnosis São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Social Dentistry and Pediatric Dentistry São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Biosciences and Oral Diagnosis São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Social Dentistry and Pediatric Dentistry São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPUniversidade Estadual Paulista (UNESP)Salvia, Ana Carolina Rodrigues Danzi [UNESP]Teodoro, Guilherme Rodrigues [UNESP]Balducci, Ivan [UNESP]Koga-Ito, Cristiane Yumi [UNESP]de Oliveira, Simone Helena Gonçalves [UNESP]2022-04-28T22:02:32Z2022-04-28T22:02:32Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23-27http://dx.doi.org/10.1590/S1806-83242011000100005Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011.1807-31071806-8324http://hdl.handle.net/11449/22624310.1590/S1806-832420110001000052-s2.0-79952164521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2022-04-28T22:02:32Zoai:repositorio.unesp.br:11449/226243Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:04:16.307657Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
title |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
spellingShingle |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones Salvia, Ana Carolina Rodrigues Danzi [UNESP] Disinfection Endodontics Microbiology |
title_short |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
title_full |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
title_fullStr |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
title_full_unstemmed |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
title_sort |
Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones |
author |
Salvia, Ana Carolina Rodrigues Danzi [UNESP] |
author_facet |
Salvia, Ana Carolina Rodrigues Danzi [UNESP] Teodoro, Guilherme Rodrigues [UNESP] Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] de Oliveira, Simone Helena Gonçalves [UNESP] |
author_role |
author |
author2 |
Teodoro, Guilherme Rodrigues [UNESP] Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] de Oliveira, Simone Helena Gonçalves [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Salvia, Ana Carolina Rodrigues Danzi [UNESP] Teodoro, Guilherme Rodrigues [UNESP] Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] de Oliveira, Simone Helena Gonçalves [UNESP] |
dc.subject.por.fl_str_mv |
Disinfection Endodontics Microbiology |
topic |
Disinfection Endodontics Microbiology |
description |
The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2022-04-28T22:02:32Z 2022-04-28T22:02:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242011000100005 Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011. 1807-3107 1806-8324 http://hdl.handle.net/11449/226243 10.1590/S1806-83242011000100005 2-s2.0-79952164521 |
url |
http://dx.doi.org/10.1590/S1806-83242011000100005 http://hdl.handle.net/11449/226243 |
identifier_str_mv |
Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011. 1807-3107 1806-8324 10.1590/S1806-83242011000100005 2-s2.0-79952164521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
23-27 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129280297664512 |