Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

Detalhes bibliográficos
Autor(a) principal: Salvia, Ana Carolina Rodrigues Danzi [UNESP]
Data de Publicação: 2011
Outros Autores: Teodoro, Guilherme Rodrigues [UNESP], Balducci, Ivan [UNESP], Koga-Ito, Cristiane Yumi [UNESP], de Oliveira, Simone Helena Gonçalves [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1806-83242011000100005
http://hdl.handle.net/11449/226243
Resumo: The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.
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spelling Effectiveness of 2% peracetic acid for the disinfection of gutta-percha conesDisinfectionEndodonticsMicrobiologyThe aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.Department of Biosciences and Oral Diagnosis São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Social Dentistry and Pediatric Dentistry São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Biosciences and Oral Diagnosis São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPDepartment of Social Dentistry and Pediatric Dentistry São José dos Campos Dental School University Estadual Paulista (UNESP), São José dos Campos, SPUniversidade Estadual Paulista (UNESP)Salvia, Ana Carolina Rodrigues Danzi [UNESP]Teodoro, Guilherme Rodrigues [UNESP]Balducci, Ivan [UNESP]Koga-Ito, Cristiane Yumi [UNESP]de Oliveira, Simone Helena Gonçalves [UNESP]2022-04-28T22:02:32Z2022-04-28T22:02:32Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23-27http://dx.doi.org/10.1590/S1806-83242011000100005Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011.1807-31071806-8324http://hdl.handle.net/11449/22624310.1590/S1806-832420110001000052-s2.0-79952164521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2022-04-28T22:02:32Zoai:repositorio.unesp.br:11449/226243Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:04:16.307657Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
title Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
spellingShingle Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
Salvia, Ana Carolina Rodrigues Danzi [UNESP]
Disinfection
Endodontics
Microbiology
title_short Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
title_full Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
title_fullStr Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
title_full_unstemmed Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
title_sort Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
author Salvia, Ana Carolina Rodrigues Danzi [UNESP]
author_facet Salvia, Ana Carolina Rodrigues Danzi [UNESP]
Teodoro, Guilherme Rodrigues [UNESP]
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
de Oliveira, Simone Helena Gonçalves [UNESP]
author_role author
author2 Teodoro, Guilherme Rodrigues [UNESP]
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
de Oliveira, Simone Helena Gonçalves [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Salvia, Ana Carolina Rodrigues Danzi [UNESP]
Teodoro, Guilherme Rodrigues [UNESP]
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
de Oliveira, Simone Helena Gonçalves [UNESP]
dc.subject.por.fl_str_mv Disinfection
Endodontics
Microbiology
topic Disinfection
Endodontics
Microbiology
description The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2022-04-28T22:02:32Z
2022-04-28T22:02:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1806-83242011000100005
Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011.
1807-3107
1806-8324
http://hdl.handle.net/11449/226243
10.1590/S1806-83242011000100005
2-s2.0-79952164521
url http://dx.doi.org/10.1590/S1806-83242011000100005
http://hdl.handle.net/11449/226243
identifier_str_mv Brazilian Oral Research, v. 25, n. 1, p. 23-27, 2011.
1807-3107
1806-8324
10.1590/S1806-83242011000100005
2-s2.0-79952164521
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Oral Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 23-27
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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