A proporção de plasma rico em plaquetas/enxerto ósseo autógeno pode influenciar o reparo ósseo de defeito de tamanho crítico: estudo radiográfico em calvária de ratos

• Autor(a) principal: Nagata, Maria José Hitomi [UNESP]
• Data de Publicação: 2011
• Outros Autores: Messora, Michel Reis, Campos, Natalia de [UNESP], Pola, Natalia Marcumini [UNESP], Salzedas, Leda Maria Pescinini [UNESP], Bomfim, Suely Regina Mogami [UNESP], Faleiros, Paula Lazilha [UNESP], Santinoni, Carolina dos Santos [UNESP], Bosco, Álvaro Francisco [UNESP], Garcia, Valdir Gouveia [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://revodontolunesp.com.br/article/51ae4aa81ef1faca3d003852; http://hdl.handle.net/11449/133202
• topic: Bone regeneration; Growth factors; Platelets; Radiography; Regeneração óssea; Fator de crescimento; Plaquetas; Radiografia

Introduction: It has been suggested that bone grafts combined with platelet rich plasma (PRP) present greater density than bone grafts without PRP after healing process. However, this result may depend on proportion PRP/autogenous bone (AB) graft used. Objective: The purpose of this study was to analyze radiographically the influence of the proportion PRP/particulate autogenous bone (AB) graft on bone healing in surgically created criticalsize defects (CSD) in rat calvaria. Material and method: 50 rats were divided into 5 groups: C, AB, AB/PRP-50, AB/PRP-100 and AB/PRP-150. A 5 mm diameter CSD was created in the calvarium of each animal. In Group C the defect was filled by blood clot only. In Group AB the defect was filled with 0.01 mL of AB graft. In groups AB/ RP-50, AB/PRP-100 and AB/PRP-150 the defects were filled with 0.01 mL of AB graft combined with 50, 100 and 150 µL of PRP, respectively. All animals were euthanized at 30 days post-operative. Standardized radiographic images of the rat calvaria were obtained using Digora System (Soredex, Finland). The images were evaluated by three examiners using scores that indicated the percentage of radiopacity of the defect. The obtained data were subjected to statistical analysis (Kruskal-Wallis test, p < 0.05). Result: Group C presented radiopacity significantly lower than groups AB, AB/PRP-50, AB/PRP-100 and AB/PRP-150. Group AB/PRP-50 showed radiopacity significantly greater than groups AB/PRP-100 and AB. Conclusion: It can be concluded that the proportion PRP/AB graft influences bone healing in CSD in rat calvaria.