Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s40858-020-00373-6 http://hdl.handle.net/11449/200623 |
Resumo: | The use of resistance inducers may be a tool to assist in the management of citrus diseases. However, knowledge about how some inducers, such as the benzothiadiazoles, promote gene expression from the pathways involved in induced resistance and alter plant development are lacking. In this work, we evaluated the effect of the acibenzolar-S-methyl (ASM) on gene expression from the biosynthetic pathways of salicylic (SA) and jasmonic (JA) acids, as well as in physiological parameters of young sweet orange trees. After a single ASM application, gene expression assessed during six time points revealed no significant changes in the main pathway for SA synthesis: isochorismate synthases (ISC) 1 and ICS2 genes were up-regulated only at 6 days after treatment (dat). However, an increase in phenylalanine ammonia lyase (PAL) was observed. Salicylic acid carboxymethyl transferase (BSMT1) and salicylic acid glucosyltransferase I (SAGT1) were up-regulated, indicating expression of genes whose products modify SA. Pointedly up-regulation of lipoxygenase (LOX) and both up- and down-regulation of allene oxide synthase (AOS) and allene oxide cyclase (AOC) in biosynthetic pathways of JA was observed, while jasmonic acid methyl transferase (JMT), whose product modify JA, was consistently induced from three to 13 dat. Besides an increase in SA and JA modification enzymes, ASM reduced photosynthetic rate in treated plants, without affecting the stomatal conductance and the transpiration rate. Our results reveal how the SA and JA pathways are regulated soon after ASM treatment, in particular the up-regulation of BSTM1, SAGT1 and JMT. |
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Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methylHuanglongbingManagementResistance inducersSignaling compoundThe use of resistance inducers may be a tool to assist in the management of citrus diseases. However, knowledge about how some inducers, such as the benzothiadiazoles, promote gene expression from the pathways involved in induced resistance and alter plant development are lacking. In this work, we evaluated the effect of the acibenzolar-S-methyl (ASM) on gene expression from the biosynthetic pathways of salicylic (SA) and jasmonic (JA) acids, as well as in physiological parameters of young sweet orange trees. After a single ASM application, gene expression assessed during six time points revealed no significant changes in the main pathway for SA synthesis: isochorismate synthases (ISC) 1 and ICS2 genes were up-regulated only at 6 days after treatment (dat). However, an increase in phenylalanine ammonia lyase (PAL) was observed. Salicylic acid carboxymethyl transferase (BSMT1) and salicylic acid glucosyltransferase I (SAGT1) were up-regulated, indicating expression of genes whose products modify SA. Pointedly up-regulation of lipoxygenase (LOX) and both up- and down-regulation of allene oxide synthase (AOS) and allene oxide cyclase (AOC) in biosynthetic pathways of JA was observed, while jasmonic acid methyl transferase (JMT), whose product modify JA, was consistently induced from three to 13 dat. Besides an increase in SA and JA modification enzymes, ASM reduced photosynthetic rate in treated plants, without affecting the stomatal conductance and the transpiration rate. Our results reveal how the SA and JA pathways are regulated soon after ASM treatment, in particular the up-regulation of BSTM1, SAGT1 and JMT.Centro de Ciências Agrárias Universidade Federal de Santa Catarina - UFSCFundo de Defesa de Citricultura - Fundecitrus, Vila MelhadoBiotecnologia Instituto de Química (Programa de pós-graduação em Biotecnologia) Universidade Estadual Paulista “Júlio de Mesquita Filho” - UnespBiotecnologia Instituto de Química (Programa de pós-graduação em Biotecnologia) Universidade Estadual Paulista “Júlio de Mesquita Filho” - UnespUniversidade Federal de Santa Catarina (UFSC)Fundo de Defesa de Citricultura - FundecitrusUniversidade Estadual Paulista (Unesp)Darolt, Josiane Cecília [UNESP]Fassini, Camila GiácomoWulff, Nelson Arno [UNESP]Di Piero, Robson Marcelo2020-12-12T02:11:35Z2020-12-12T02:11:35Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s40858-020-00373-6Tropical Plant Pathology.1983-20521982-5676http://hdl.handle.net/11449/20062310.1007/s40858-020-00373-62-s2.0-85086704179Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Plant Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T14:48:21Zoai:repositorio.unesp.br:11449/200623Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:48:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
title |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
spellingShingle |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl Darolt, Josiane Cecília [UNESP] Huanglongbing Management Resistance inducers Signaling compound |
title_short |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
title_full |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
title_fullStr |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
title_full_unstemmed |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
title_sort |
Gene expression of salicylic acid and jasmonic acid pathways and photosynthesis parameters of sweet orange trees in response to acibenzolar-S-methyl |
author |
Darolt, Josiane Cecília [UNESP] |
author_facet |
Darolt, Josiane Cecília [UNESP] Fassini, Camila Giácomo Wulff, Nelson Arno [UNESP] Di Piero, Robson Marcelo |
author_role |
author |
author2 |
Fassini, Camila Giácomo Wulff, Nelson Arno [UNESP] Di Piero, Robson Marcelo |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Santa Catarina (UFSC) Fundo de Defesa de Citricultura - Fundecitrus Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Darolt, Josiane Cecília [UNESP] Fassini, Camila Giácomo Wulff, Nelson Arno [UNESP] Di Piero, Robson Marcelo |
dc.subject.por.fl_str_mv |
Huanglongbing Management Resistance inducers Signaling compound |
topic |
Huanglongbing Management Resistance inducers Signaling compound |
description |
The use of resistance inducers may be a tool to assist in the management of citrus diseases. However, knowledge about how some inducers, such as the benzothiadiazoles, promote gene expression from the pathways involved in induced resistance and alter plant development are lacking. In this work, we evaluated the effect of the acibenzolar-S-methyl (ASM) on gene expression from the biosynthetic pathways of salicylic (SA) and jasmonic (JA) acids, as well as in physiological parameters of young sweet orange trees. After a single ASM application, gene expression assessed during six time points revealed no significant changes in the main pathway for SA synthesis: isochorismate synthases (ISC) 1 and ICS2 genes were up-regulated only at 6 days after treatment (dat). However, an increase in phenylalanine ammonia lyase (PAL) was observed. Salicylic acid carboxymethyl transferase (BSMT1) and salicylic acid glucosyltransferase I (SAGT1) were up-regulated, indicating expression of genes whose products modify SA. Pointedly up-regulation of lipoxygenase (LOX) and both up- and down-regulation of allene oxide synthase (AOS) and allene oxide cyclase (AOC) in biosynthetic pathways of JA was observed, while jasmonic acid methyl transferase (JMT), whose product modify JA, was consistently induced from three to 13 dat. Besides an increase in SA and JA modification enzymes, ASM reduced photosynthetic rate in treated plants, without affecting the stomatal conductance and the transpiration rate. Our results reveal how the SA and JA pathways are regulated soon after ASM treatment, in particular the up-regulation of BSTM1, SAGT1 and JMT. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:11:35Z 2020-12-12T02:11:35Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s40858-020-00373-6 Tropical Plant Pathology. 1983-2052 1982-5676 http://hdl.handle.net/11449/200623 10.1007/s40858-020-00373-6 2-s2.0-85086704179 |
url |
http://dx.doi.org/10.1007/s40858-020-00373-6 http://hdl.handle.net/11449/200623 |
identifier_str_mv |
Tropical Plant Pathology. 1983-2052 1982-5676 10.1007/s40858-020-00373-6 2-s2.0-85086704179 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tropical Plant Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965421505347584 |