Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/microorganisms9051008 http://hdl.handle.net/11449/207702 |
Resumo: | Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes. |
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Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilationBiodegradationMelanized fungiTolueneEnvironmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center for the Study of Social Insects São Paulo State University (UNESP)GIRO Institute of Agrifood Research and Technology (IRTA), Torre MarimonBasic Pathology Department Federal University of Paraná (UFPR)Division of Microbial Resources CPQBA University of Campinas (Unicamp)Center for the Study of Social Insects São Paulo State University (UNESP)CAPES: 2013/01556-2CNPq: 2013/01556-2FAPESP: 2013/01556-2CNPq: 312811/2018-7.Universidade Estadual Paulista (Unesp)Institute of Agrifood Research and Technology (IRTA)Universidade Federal do Paraná (UFPR)Universidade Estadual de Campinas (UNICAMP)Baron, Noemi Carla [UNESP]Pagnocca, Fernando Carlos [UNESP]de Aquino Otsuka, Ayumi [UNESP]Prenafeta-boldú, Francesc XavierVicente, Vânia AparecidaAttili de Angelis, Derlene [UNESP]2021-06-25T10:59:32Z2021-06-25T10:59:32Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms9051008Microorganisms, v. 9, n. 5, 2021.2076-2607http://hdl.handle.net/11449/20770210.3390/microorganisms90510082-s2.0-85105392660Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2024-04-11T14:57:11Zoai:repositorio.unesp.br:11449/207702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:01:28.610513Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
title |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
spellingShingle |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation Baron, Noemi Carla [UNESP] Biodegradation Melanized fungi Toluene |
title_short |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
title_full |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
title_fullStr |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
title_full_unstemmed |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
title_sort |
Black fungi and hydrocarbons: An environmental survey for alkylbenzene assimilation |
author |
Baron, Noemi Carla [UNESP] |
author_facet |
Baron, Noemi Carla [UNESP] Pagnocca, Fernando Carlos [UNESP] de Aquino Otsuka, Ayumi [UNESP] Prenafeta-boldú, Francesc Xavier Vicente, Vânia Aparecida Attili de Angelis, Derlene [UNESP] |
author_role |
author |
author2 |
Pagnocca, Fernando Carlos [UNESP] de Aquino Otsuka, Ayumi [UNESP] Prenafeta-boldú, Francesc Xavier Vicente, Vânia Aparecida Attili de Angelis, Derlene [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute of Agrifood Research and Technology (IRTA) Universidade Federal do Paraná (UFPR) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Baron, Noemi Carla [UNESP] Pagnocca, Fernando Carlos [UNESP] de Aquino Otsuka, Ayumi [UNESP] Prenafeta-boldú, Francesc Xavier Vicente, Vânia Aparecida Attili de Angelis, Derlene [UNESP] |
dc.subject.por.fl_str_mv |
Biodegradation Melanized fungi Toluene |
topic |
Biodegradation Melanized fungi Toluene |
description |
Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:32Z 2021-06-25T10:59:32Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/microorganisms9051008 Microorganisms, v. 9, n. 5, 2021. 2076-2607 http://hdl.handle.net/11449/207702 10.3390/microorganisms9051008 2-s2.0-85105392660 |
url |
http://dx.doi.org/10.3390/microorganisms9051008 http://hdl.handle.net/11449/207702 |
identifier_str_mv |
Microorganisms, v. 9, n. 5, 2021. 2076-2607 10.3390/microorganisms9051008 2-s2.0-85105392660 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128886147383296 |