Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-021-92425-3 http://hdl.handle.net/11449/221836 |
Resumo: | Sea lice (Caligus rogercresseyi) is an ectoparasite which causes major production losses in the salmon aquaculture industry worldwide. Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) are two of the most susceptible salmonid species to sea lice infestation. The objectives of this study were to: (1) identify genomic regions associated with resistance to Caligus rogercresseyi in Atlantic salmon and rainbow trout by performing single-step Genome-Wide Association studies (ssGWAS), and (2) identify candidate genes related to trait variation based on exploring orthologous genes within the associated regions across species. A total of 2626 Atlantic salmon and 2643 rainbow trout were challenged and genotyped with 50 K and 57 K SNP panels, respectively. We ran two independent ssGWAS for sea lice resistance on each species and identified 7 and 13 regions explaining more than 1% of the genetic variance for the trait, with the most important regions explaining 3% and 2.7% for Atlantic salmon and rainbow trout, respectively. We identified genes associated with immune response, cytoskeleton function, and cell migration when focusing on important genomic regions for each species. Moreover, we found 15 common orthogroups which were present in more than one associated genomic region, within- or between-species; however, only one orthogroup showed a clear potential biological relevance in the response against sea lice. For instance, dual-specificity protein phosphatase 10-like (dusp10) and dual-specificity protein phosphatase 8 (dusp8) were found in genomic regions associated with lice density in Atlantic salmon and rainbow trout, respectively. Dusp10 and dusp8 are modulators of the MAPK pathway and might be involved in the differences of the inflammation response between lice resistant and susceptible fish from both species. Our results provide further knowledge on candidate genes related to sea lice resistance and may help establish better control for sea lice in fish populations. |
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Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow troutSea lice (Caligus rogercresseyi) is an ectoparasite which causes major production losses in the salmon aquaculture industry worldwide. Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) are two of the most susceptible salmonid species to sea lice infestation. The objectives of this study were to: (1) identify genomic regions associated with resistance to Caligus rogercresseyi in Atlantic salmon and rainbow trout by performing single-step Genome-Wide Association studies (ssGWAS), and (2) identify candidate genes related to trait variation based on exploring orthologous genes within the associated regions across species. A total of 2626 Atlantic salmon and 2643 rainbow trout were challenged and genotyped with 50 K and 57 K SNP panels, respectively. We ran two independent ssGWAS for sea lice resistance on each species and identified 7 and 13 regions explaining more than 1% of the genetic variance for the trait, with the most important regions explaining 3% and 2.7% for Atlantic salmon and rainbow trout, respectively. We identified genes associated with immune response, cytoskeleton function, and cell migration when focusing on important genomic regions for each species. Moreover, we found 15 common orthogroups which were present in more than one associated genomic region, within- or between-species; however, only one orthogroup showed a clear potential biological relevance in the response against sea lice. For instance, dual-specificity protein phosphatase 10-like (dusp10) and dual-specificity protein phosphatase 8 (dusp8) were found in genomic regions associated with lice density in Atlantic salmon and rainbow trout, respectively. Dusp10 and dusp8 are modulators of the MAPK pathway and might be involved in the differences of the inflammation response between lice resistant and susceptible fish from both species. Our results provide further knowledge on candidate genes related to sea lice resistance and may help establish better control for sea lice in fish populations.Universidad de Santiago de ChileFacultad de Ciencias Veterinarias y Pecuarias Universidad de Chile, Av. Santa Rosa 11735, La PintanaThe Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Easter BushFisheries and Oceans Canada, 4160 Marine DriveEscuela de Medicina Veterinaria Facultad de Ciencias de la Vida Universidad Andres BelloSchool of Agricultural and Veterinary Sciences UNESP-Sao Paulo State UniversityAquainnovo, Cardonal S/NNúcleo Milenio INVASALCenter for Research and Innovation in Aquaculture (CRIA) Universidad de ChileSchool of Agricultural and Veterinary Sciences UNESP-Sao Paulo State UniversityUniversidad de Santiago de Chile: MR/N026144/1Universidad de ChileUniversity of Edinburgh Easter BushFisheries and Oceans CanadaUniversidad Andres BelloUniversidade Estadual Paulista (UNESP)AquainnovoNúcleo Milenio INVASALCáceres, PabloBarría, AgustínChristensen, Kris A.Bassini, Liane N.Correa, KatharinaGarcia, Baltasar [UNESP]Lhorente, Jean P.Yáñez, José M.2022-04-28T19:40:53Z2022-04-28T19:40:53Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-92425-3Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/22183610.1038/s41598-021-92425-32-s2.0-85108623382Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:40:53Zoai:repositorio.unesp.br:11449/221836Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:58:12.353313Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
title |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
spellingShingle |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout Cáceres, Pablo |
title_short |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
title_full |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
title_fullStr |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
title_full_unstemmed |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
title_sort |
Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout |
author |
Cáceres, Pablo |
author_facet |
Cáceres, Pablo Barría, Agustín Christensen, Kris A. Bassini, Liane N. Correa, Katharina Garcia, Baltasar [UNESP] Lhorente, Jean P. Yáñez, José M. |
author_role |
author |
author2 |
Barría, Agustín Christensen, Kris A. Bassini, Liane N. Correa, Katharina Garcia, Baltasar [UNESP] Lhorente, Jean P. Yáñez, José M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidad de Chile University of Edinburgh Easter Bush Fisheries and Oceans Canada Universidad Andres Bello Universidade Estadual Paulista (UNESP) Aquainnovo Núcleo Milenio INVASAL |
dc.contributor.author.fl_str_mv |
Cáceres, Pablo Barría, Agustín Christensen, Kris A. Bassini, Liane N. Correa, Katharina Garcia, Baltasar [UNESP] Lhorente, Jean P. Yáñez, José M. |
description |
Sea lice (Caligus rogercresseyi) is an ectoparasite which causes major production losses in the salmon aquaculture industry worldwide. Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) are two of the most susceptible salmonid species to sea lice infestation. The objectives of this study were to: (1) identify genomic regions associated with resistance to Caligus rogercresseyi in Atlantic salmon and rainbow trout by performing single-step Genome-Wide Association studies (ssGWAS), and (2) identify candidate genes related to trait variation based on exploring orthologous genes within the associated regions across species. A total of 2626 Atlantic salmon and 2643 rainbow trout were challenged and genotyped with 50 K and 57 K SNP panels, respectively. We ran two independent ssGWAS for sea lice resistance on each species and identified 7 and 13 regions explaining more than 1% of the genetic variance for the trait, with the most important regions explaining 3% and 2.7% for Atlantic salmon and rainbow trout, respectively. We identified genes associated with immune response, cytoskeleton function, and cell migration when focusing on important genomic regions for each species. Moreover, we found 15 common orthogroups which were present in more than one associated genomic region, within- or between-species; however, only one orthogroup showed a clear potential biological relevance in the response against sea lice. For instance, dual-specificity protein phosphatase 10-like (dusp10) and dual-specificity protein phosphatase 8 (dusp8) were found in genomic regions associated with lice density in Atlantic salmon and rainbow trout, respectively. Dusp10 and dusp8 are modulators of the MAPK pathway and might be involved in the differences of the inflammation response between lice resistant and susceptible fish from both species. Our results provide further knowledge on candidate genes related to sea lice resistance and may help establish better control for sea lice in fish populations. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-28T19:40:53Z 2022-04-28T19:40:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-021-92425-3 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/221836 10.1038/s41598-021-92425-3 2-s2.0-85108623382 |
url |
http://dx.doi.org/10.1038/s41598-021-92425-3 http://hdl.handle.net/11449/221836 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-021-92425-3 2-s2.0-85108623382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128297617326080 |