Antimicrobial photodynamic action on dentin using a light-emitting diode light source
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/pho.2007.2149 http://hdl.handle.net/11449/42339 |
Resumo: | Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used. |
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Repositório Institucional da UNESP |
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Antimicrobial photodynamic action on dentin using a light-emitting diode light sourceObjective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CePOFConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP, Fac Odontol Araraquara, Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilNIST, Gaithersburg, MD 20899 USAUniv São Paulo, IFSC, Sao Carlos, SP, BrazilUNESP, Fac Odontol Araraquara, Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilMary Ann Liebert, Inc.Universidade Estadual Paulista (Unesp)NISTUniversidade de São Paulo (USP)Giusti, Jucaira S. M. [UNESP]Santos-Pinto, Lourdes Aparecida Martins dos [UNESP]Pizzolito, Antonio C. [UNESP]Helmerson, KristianCarvalho-Filho, EuricoKurachi, CristinaBagnato, Vanderlei S.2014-05-20T15:33:51Z2014-05-20T15:33:51Z2008-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article281-287application/pdfhttp://dx.doi.org/10.1089/pho.2007.2149Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008.1549-5418http://hdl.handle.net/11449/4233910.1089/pho.2007.2149WOS:000259126200002WOS000259126200002.pdf0000-0003-2386-842XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotomedicine and Laser Surgery1.6200,443info:eu-repo/semantics/openAccess2024-09-26T14:21:05Zoai:repositorio.unesp.br:11449/42339Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
title |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
spellingShingle |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source Giusti, Jucaira S. M. [UNESP] |
title_short |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
title_full |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
title_fullStr |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
title_full_unstemmed |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
title_sort |
Antimicrobial photodynamic action on dentin using a light-emitting diode light source |
author |
Giusti, Jucaira S. M. [UNESP] |
author_facet |
Giusti, Jucaira S. M. [UNESP] Santos-Pinto, Lourdes Aparecida Martins dos [UNESP] Pizzolito, Antonio C. [UNESP] Helmerson, Kristian Carvalho-Filho, Eurico Kurachi, Cristina Bagnato, Vanderlei S. |
author_role |
author |
author2 |
Santos-Pinto, Lourdes Aparecida Martins dos [UNESP] Pizzolito, Antonio C. [UNESP] Helmerson, Kristian Carvalho-Filho, Eurico Kurachi, Cristina Bagnato, Vanderlei S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) NIST Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Giusti, Jucaira S. M. [UNESP] Santos-Pinto, Lourdes Aparecida Martins dos [UNESP] Pizzolito, Antonio C. [UNESP] Helmerson, Kristian Carvalho-Filho, Eurico Kurachi, Cristina Bagnato, Vanderlei S. |
description |
Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-01 2014-05-20T15:33:51Z 2014-05-20T15:33:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/pho.2007.2149 Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008. 1549-5418 http://hdl.handle.net/11449/42339 10.1089/pho.2007.2149 WOS:000259126200002 WOS000259126200002.pdf 0000-0003-2386-842X |
url |
http://dx.doi.org/10.1089/pho.2007.2149 http://hdl.handle.net/11449/42339 |
identifier_str_mv |
Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008. 1549-5418 10.1089/pho.2007.2149 WOS:000259126200002 WOS000259126200002.pdf 0000-0003-2386-842X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photomedicine and Laser Surgery 1.620 0,443 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
281-287 application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546385077698560 |