Antimicrobial photodynamic action on dentin using a light-emitting diode light source

Detalhes bibliográficos
Autor(a) principal: Giusti, Jucaira S. M. [UNESP]
Data de Publicação: 2008
Outros Autores: Santos-Pinto, Lourdes Aparecida Martins dos [UNESP], Pizzolito, Antonio C. [UNESP], Helmerson, Kristian, Carvalho-Filho, Eurico, Kurachi, Cristina, Bagnato, Vanderlei S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1089/pho.2007.2149
http://hdl.handle.net/11449/42339
Resumo: Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.
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spelling Antimicrobial photodynamic action on dentin using a light-emitting diode light sourceObjective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CePOFConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP, Fac Odontol Araraquara, Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilNIST, Gaithersburg, MD 20899 USAUniv São Paulo, IFSC, Sao Carlos, SP, BrazilUNESP, Fac Odontol Araraquara, Araraquara, SP, BrazilUNESP, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilMary Ann Liebert, Inc.Universidade Estadual Paulista (Unesp)NISTUniversidade de São Paulo (USP)Giusti, Jucaira S. M. [UNESP]Santos-Pinto, Lourdes Aparecida Martins dos [UNESP]Pizzolito, Antonio C. [UNESP]Helmerson, KristianCarvalho-Filho, EuricoKurachi, CristinaBagnato, Vanderlei S.2014-05-20T15:33:51Z2014-05-20T15:33:51Z2008-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article281-287application/pdfhttp://dx.doi.org/10.1089/pho.2007.2149Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008.1549-5418http://hdl.handle.net/11449/4233910.1089/pho.2007.2149WOS:000259126200002WOS000259126200002.pdf0000-0003-2386-842XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotomedicine and Laser Surgery1.6200,443info:eu-repo/semantics/openAccess2024-09-26T14:21:05Zoai:repositorio.unesp.br:11449/42339Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial photodynamic action on dentin using a light-emitting diode light source
title Antimicrobial photodynamic action on dentin using a light-emitting diode light source
spellingShingle Antimicrobial photodynamic action on dentin using a light-emitting diode light source
Giusti, Jucaira S. M. [UNESP]
title_short Antimicrobial photodynamic action on dentin using a light-emitting diode light source
title_full Antimicrobial photodynamic action on dentin using a light-emitting diode light source
title_fullStr Antimicrobial photodynamic action on dentin using a light-emitting diode light source
title_full_unstemmed Antimicrobial photodynamic action on dentin using a light-emitting diode light source
title_sort Antimicrobial photodynamic action on dentin using a light-emitting diode light source
author Giusti, Jucaira S. M. [UNESP]
author_facet Giusti, Jucaira S. M. [UNESP]
Santos-Pinto, Lourdes Aparecida Martins dos [UNESP]
Pizzolito, Antonio C. [UNESP]
Helmerson, Kristian
Carvalho-Filho, Eurico
Kurachi, Cristina
Bagnato, Vanderlei S.
author_role author
author2 Santos-Pinto, Lourdes Aparecida Martins dos [UNESP]
Pizzolito, Antonio C. [UNESP]
Helmerson, Kristian
Carvalho-Filho, Eurico
Kurachi, Cristina
Bagnato, Vanderlei S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
NIST
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Giusti, Jucaira S. M. [UNESP]
Santos-Pinto, Lourdes Aparecida Martins dos [UNESP]
Pizzolito, Antonio C. [UNESP]
Helmerson, Kristian
Carvalho-Filho, Eurico
Kurachi, Cristina
Bagnato, Vanderlei S.
description Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin.Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2).Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem.Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
2014-05-20T15:33:51Z
2014-05-20T15:33:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1089/pho.2007.2149
Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008.
1549-5418
http://hdl.handle.net/11449/42339
10.1089/pho.2007.2149
WOS:000259126200002
WOS000259126200002.pdf
0000-0003-2386-842X
url http://dx.doi.org/10.1089/pho.2007.2149
http://hdl.handle.net/11449/42339
identifier_str_mv Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 4, p. 281-287, 2008.
1549-5418
10.1089/pho.2007.2149
WOS:000259126200002
WOS000259126200002.pdf
0000-0003-2386-842X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photomedicine and Laser Surgery
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0,443
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 281-287
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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