Karyotype Evolution in Triatominae (Hemiptera, Reduviidae): The Role of Chromosomal Rearrangements in the Diversification of Chagas Disease Vectors

• Autor(a) principal: Reis, Yago Visinho dos [UNESP]
• Data de Publicação: 2023
• Outros Autores: de Oliveira, Jader, Madeira, Fernanda Fernandez [UNESP], Ravazi, Amanda [UNESP], Oliveira, Ana Beatriz Bortolozo de [UNESP], Bittinelli, Isadora da Silva [UNESP], Delgado, Luiza Maria Grzyb [UNESP], de Azeredo-Oliveira, Maria Tercília Vilela [UNESP], Rosa, João Aristeu da [UNESP], Galvão, Cleber, Alevi, Kaio Cesar Chaboli [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.3390/ijms24076350; http://hdl.handle.net/11449/249852
• topic: ChromoSSE; experimental crosses; karyotypic isolation; phylogenetic analysis

Several cytogenetic studies have already been performed in Triatominae, such that different karyotypes could be characterized (ranging from 2n = 21 to 25 chromosomes), being the changes in the number of chromosomes related mainly to fusion and fission events. These changes have been associated with reproductive isolation and speciation events in other insect groups. Thus, we evaluated whether different karyotypes could act in the reproductive isolation of triatomines and we analyzed how the events of karyotypic evolution occurred along the diversification of these vectors. For this, experimental crosses were carried out between triatomine species with different karyotypes. Furthermore, based on a phylogeny with 88 triatomine taxa (developed with different molecular markers), a reconstruction of ancestral karyotypes and of anagenetic and cladogenetic events related to karyotypic alterations was performed through the ChromoSSE chromosomal evolution model. All crosses performed did not result in hybrids (prezygotic isolation in both directions). Our modeling results suggest that during Triatominae diversification, at least nine cladogenetic events may be associated with karyotype change. Thus, we emphasize that these alterations in the number of chromosomes can act as a prezygotic barrier in Triatominae (karyotypic isolation), being important evolutionary events during the diversification of the species of Chagas disease vectors.