Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2014001000016 http://hdl.handle.net/11449/114156 |
Resumo: | The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes. |
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Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus)Produção lacrimal, pressão intraocular, microbiota, citologia e histologia conjuntival de coelhos Nova ZelândiaProdução lacrimalpressão intraocularcoelhos Nova ZelândiaOryctolagus cuniculuslagomorfosoftalmologiateste lacrimal de Schirmertonometriatira endodôntica de papel absorventeTear productionintraocular pressureconjunctival microbiotaNew Zealand rabbitsOryctolagus cuniculuslagomorphsophthalmologySchirmer tear testtonometryendodontic paper pointThe purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.O objetivo deste estudo foi estabelecer valores de referência para testes diagnósticos oftálmicos em coelhos da raça Nova Zelândia (Oryctolagus cuniculus). 22 coelhos, machos, adultos foram utilizados. Foi mensurada a produção lacrimal através do teste lacrimal de Shirmer 1 (TLS1) e da Tira endodôntica de papel absorvente (EAPPTT) de duas marcas comerciais distintas. Tonometria de aplanação, identificação da microbiota conjuntival, , citologia e histologia conjuntival também foram realizadas. A média e desvio padrão do TLS1, EAPPTT1, EAPPTT2 e pressão intraocular foi 7,27±2,51 mm/min, 12,43±1,69 mm/min, 15,24±2,07 mm/min e 12,89±2,80 mmHg, respectivamente. Staphylococcus epidermidis, Staphylococcus sp. e Bacillus sp. mostraram-se predominantes. A citologia conjuntival evidenciou a presença de células epiteliais colunares, células escamosas superficiais queratinizadas, linfócitos, heterofilos, células sanguíneas, muco e bactérias. A histologia revelou epitélio estratificado caracterizado pela presença de células epiteliais colunares com grande número de células caliciformes. Os achados deste estudo poderão ser utilizados com fins terapêuticos ou experimentais.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal da Bahia (UFBA) Escola de Medicina Veterinária e Zootecnia Departamento de Anatomia, Patologia e ClínicasUFBA Escola de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e VeterináriasColégio Brasileiro de Patologia Animal - CBPAUniversidade Federal da Bahia (UFBA)Universidade Federal da Bahia (UFBA)Universidade Estadual Paulista (Unesp)Oriá, Arianne P.Gomes Junior, Deusdete C.Arraes, Elane A.Estrela-lima, AlessandraPinna, Melissa H.Meneses, Íris D.s.Martins Filho, Emanoel F. [UNESP]2015-02-02T12:39:17Z2015-02-02T12:39:17Z2014-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1024-1028application/pdfhttp://dx.doi.org/10.1590/S0100-736X2014001000016Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 10, p. 1024-1028, 2014.0100-736Xhttp://hdl.handle.net/11449/11415610.1590/S0100-736X2014001000016S0100-736X2014001000016S0100-736X2014001000016.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinária Brasileira0.385info:eu-repo/semantics/openAccess2023-12-01T06:15:33Zoai:repositorio.unesp.br:11449/114156Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:44.682946Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) Produção lacrimal, pressão intraocular, microbiota, citologia e histologia conjuntival de coelhos Nova Zelândia |
title |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
spellingShingle |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) Oriá, Arianne P. Produção lacrimal pressão intraocular coelhos Nova Zelândia Oryctolagus cuniculus lagomorfos oftalmologia teste lacrimal de Schirmer tonometria tira endodôntica de papel absorvente Tear production intraocular pressure conjunctival microbiota New Zealand rabbits Oryctolagus cuniculus lagomorphs ophthalmology Schirmer tear test tonometry endodontic paper point |
title_short |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
title_full |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
title_fullStr |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
title_full_unstemmed |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
title_sort |
Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) |
author |
Oriá, Arianne P. |
author_facet |
Oriá, Arianne P. Gomes Junior, Deusdete C. Arraes, Elane A. Estrela-lima, Alessandra Pinna, Melissa H. Meneses, Íris D.s. Martins Filho, Emanoel F. [UNESP] |
author_role |
author |
author2 |
Gomes Junior, Deusdete C. Arraes, Elane A. Estrela-lima, Alessandra Pinna, Melissa H. Meneses, Íris D.s. Martins Filho, Emanoel F. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal da Bahia (UFBA) Universidade Federal da Bahia (UFBA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oriá, Arianne P. Gomes Junior, Deusdete C. Arraes, Elane A. Estrela-lima, Alessandra Pinna, Melissa H. Meneses, Íris D.s. Martins Filho, Emanoel F. [UNESP] |
dc.subject.por.fl_str_mv |
Produção lacrimal pressão intraocular coelhos Nova Zelândia Oryctolagus cuniculus lagomorfos oftalmologia teste lacrimal de Schirmer tonometria tira endodôntica de papel absorvente Tear production intraocular pressure conjunctival microbiota New Zealand rabbits Oryctolagus cuniculus lagomorphs ophthalmology Schirmer tear test tonometry endodontic paper point |
topic |
Produção lacrimal pressão intraocular coelhos Nova Zelândia Oryctolagus cuniculus lagomorfos oftalmologia teste lacrimal de Schirmer tonometria tira endodôntica de papel absorvente Tear production intraocular pressure conjunctival microbiota New Zealand rabbits Oryctolagus cuniculus lagomorphs ophthalmology Schirmer tear test tonometry endodontic paper point |
description |
The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 2015-02-02T12:39:17Z 2015-02-02T12:39:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2014001000016 Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 10, p. 1024-1028, 2014. 0100-736X http://hdl.handle.net/11449/114156 10.1590/S0100-736X2014001000016 S0100-736X2014001000016 S0100-736X2014001000016.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2014001000016 http://hdl.handle.net/11449/114156 |
identifier_str_mv |
Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 10, p. 1024-1028, 2014. 0100-736X 10.1590/S0100-736X2014001000016 S0100-736X2014001000016 S0100-736X2014001000016.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinária Brasileira 0.385 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1024-1028 application/pdf |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129037518766080 |