Protective effects of desipramine on alveolar bone in experimental periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/JPER.19-0569 http://hdl.handle.net/11449/196943 |
Resumo: | Background Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis. |
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Protective effects of desipramine on alveolar bone in experimental periodontitisbone resorptioncollagendesipramineinflammationperiodontal diseasesBackground Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA)Maranhao State Research Foundation (FAPEMA, Brazil)Univ Fed Maranhao, Post Grad Program Dent, Sao Luis, Maranhao, BrazilUniv Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, Parana, BrazilFac Sci Tocantins, Araguaina, Tocantins, BrazilUniv Ceuma, Post Grad Program Dent, Sao Luis, Maranhao, BrazilUniv Fed Maranhao, Sch Med, Sao Luis, Maranhao, BrazilUniv Taubate, Nucleus Periodontal Res, Taubate, SP, BrazilSao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, BrazilNova Southeastern Univ, Coll Dent Med, Ft Lauderdale, FL 33314 USAUniv Fed Alfenas, Biol Sci Grad Program, Rua Gabriel Monteiroda Silva 700,Predio E Sala, BR-37130001 Alfenas, MG, BrazilSao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, BrazilFAPESP: 2008/00566-6CAPES: 4073/08-8National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA): R15 DE027851Maranhao State Research Foundation (FAPEMA, Brazil): 1818/2012Wiley-BlackwellUniv Fed MaranhaoUniversidade Estadual de Ponta Grossa (UEPG)Fac Sci TocantinsUniv CeumaUniv TaubateUniversidade Estadual Paulista (Unesp)Nova Southeastern UnivUniv Fed AlfenasBranco-de-Almeida, Luciana S.Franco, Gilson C. N.Castro, Myrella L.Vieira, Mayana S.Galvao-Moreira, LeonardoCortelli, Sheila C.Anbinder, Ana L. [UNESP]Kawai, ToshihisaRosalen, Pedro L.2020-12-10T20:01:09Z2020-12-10T20:01:09Z2020-06-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1002/JPER.19-0569Journal Of Periodontology. Hoboken: Wiley, 10 p., 2020.0022-3492http://hdl.handle.net/11449/19694310.1002/JPER.19-0569WOS:000537914000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Periodontologyinfo:eu-repo/semantics/openAccess2021-10-23T10:11:19Zoai:repositorio.unesp.br:11449/196943Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:24.342848Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
title |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
spellingShingle |
Protective effects of desipramine on alveolar bone in experimental periodontitis Branco-de-Almeida, Luciana S. bone resorption collagen desipramine inflammation periodontal diseases |
title_short |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
title_full |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
title_fullStr |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
title_full_unstemmed |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
title_sort |
Protective effects of desipramine on alveolar bone in experimental periodontitis |
author |
Branco-de-Almeida, Luciana S. |
author_facet |
Branco-de-Almeida, Luciana S. Franco, Gilson C. N. Castro, Myrella L. Vieira, Mayana S. Galvao-Moreira, Leonardo Cortelli, Sheila C. Anbinder, Ana L. [UNESP] Kawai, Toshihisa Rosalen, Pedro L. |
author_role |
author |
author2 |
Franco, Gilson C. N. Castro, Myrella L. Vieira, Mayana S. Galvao-Moreira, Leonardo Cortelli, Sheila C. Anbinder, Ana L. [UNESP] Kawai, Toshihisa Rosalen, Pedro L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Maranhao Universidade Estadual de Ponta Grossa (UEPG) Fac Sci Tocantins Univ Ceuma Univ Taubate Universidade Estadual Paulista (Unesp) Nova Southeastern Univ Univ Fed Alfenas |
dc.contributor.author.fl_str_mv |
Branco-de-Almeida, Luciana S. Franco, Gilson C. N. Castro, Myrella L. Vieira, Mayana S. Galvao-Moreira, Leonardo Cortelli, Sheila C. Anbinder, Ana L. [UNESP] Kawai, Toshihisa Rosalen, Pedro L. |
dc.subject.por.fl_str_mv |
bone resorption collagen desipramine inflammation periodontal diseases |
topic |
bone resorption collagen desipramine inflammation periodontal diseases |
description |
Background Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:01:09Z 2020-12-10T20:01:09Z 2020-06-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/JPER.19-0569 Journal Of Periodontology. Hoboken: Wiley, 10 p., 2020. 0022-3492 http://hdl.handle.net/11449/196943 10.1002/JPER.19-0569 WOS:000537914000001 |
url |
http://dx.doi.org/10.1002/JPER.19-0569 http://hdl.handle.net/11449/196943 |
identifier_str_mv |
Journal Of Periodontology. Hoboken: Wiley, 10 p., 2020. 0022-3492 10.1002/JPER.19-0569 WOS:000537914000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Periodontology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128619666472960 |