Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes

Detalhes bibliográficos
Autor(a) principal: Oliveira, Marcos A. [UNESP]
Data de Publicação: 2021
Outros Autores: Martinez, Emanuel R.M. [UNESP], Butzge, Arno J. [UNESP], Doretto, Lucas B. [UNESP], Ricci, Juliana M.B. [UNESP], Rodrigues, Maira S. [UNESP], Vigoya, Angel A.A. [UNESP], Gómez-González, Núria E., Stewart, Amanda B., Nóbrega, Rafael H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.gep.2021.119169
http://hdl.handle.net/11449/207396
Resumo: Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males.
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spelling Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testesanti-Müllerian hormoneCommon carpSpermatogenesisTestisAnti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males.Centers for Disease Control and PreventionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade Estadual PaulistaASCRS Research FoundationAquaculture Program (CAUNESP) São Paulo State University (UNESP), JaboticabalReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), BotucatuFaculty of Veterinary Medicine and Animal Science San Martín University Foundation (FUSM)Department of Cell Biology and Histology Faculty of Biology Universidad de Murcia IMIB-ArrixacaDepartment of Orthopaedics Muscle skeletal Research West Virginia UniversityAquaculture Program (CAUNESP) São Paulo State University (UNESP), JaboticabalReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), BotucatuUniversidade Estadual Paulista (Unesp)San Martín University Foundation (FUSM)IMIB-ArrixacaWest Virginia UniversityOliveira, Marcos A. [UNESP]Martinez, Emanuel R.M. [UNESP]Butzge, Arno J. [UNESP]Doretto, Lucas B. [UNESP]Ricci, Juliana M.B. [UNESP]Rodrigues, Maira S. [UNESP]Vigoya, Angel A.A. [UNESP]Gómez-González, Núria E.Stewart, Amanda B.Nóbrega, Rafael H. [UNESP]2021-06-25T10:54:33Z2021-06-25T10:54:33Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.gep.2021.119169Gene Expression Patterns, v. 40.1872-72981567-133Xhttp://hdl.handle.net/11449/20739610.1016/j.gep.2021.1191692-s2.0-85102054462Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGene Expression Patternsinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/207396Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:26.883971Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
title Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
spellingShingle Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
Oliveira, Marcos A. [UNESP]
anti-Müllerian hormone
Common carp
Spermatogenesis
Testis
title_short Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
title_full Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
title_fullStr Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
title_full_unstemmed Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
title_sort Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
author Oliveira, Marcos A. [UNESP]
author_facet Oliveira, Marcos A. [UNESP]
Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Rodrigues, Maira S. [UNESP]
Vigoya, Angel A.A. [UNESP]
Gómez-González, Núria E.
Stewart, Amanda B.
Nóbrega, Rafael H. [UNESP]
author_role author
author2 Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Rodrigues, Maira S. [UNESP]
Vigoya, Angel A.A. [UNESP]
Gómez-González, Núria E.
Stewart, Amanda B.
Nóbrega, Rafael H. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
San Martín University Foundation (FUSM)
IMIB-Arrixaca
West Virginia University
dc.contributor.author.fl_str_mv Oliveira, Marcos A. [UNESP]
Martinez, Emanuel R.M. [UNESP]
Butzge, Arno J. [UNESP]
Doretto, Lucas B. [UNESP]
Ricci, Juliana M.B. [UNESP]
Rodrigues, Maira S. [UNESP]
Vigoya, Angel A.A. [UNESP]
Gómez-González, Núria E.
Stewart, Amanda B.
Nóbrega, Rafael H. [UNESP]
dc.subject.por.fl_str_mv anti-Müllerian hormone
Common carp
Spermatogenesis
Testis
topic anti-Müllerian hormone
Common carp
Spermatogenesis
Testis
description Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:54:33Z
2021-06-25T10:54:33Z
2021-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.gep.2021.119169
Gene Expression Patterns, v. 40.
1872-7298
1567-133X
http://hdl.handle.net/11449/207396
10.1016/j.gep.2021.119169
2-s2.0-85102054462
url http://dx.doi.org/10.1016/j.gep.2021.119169
http://hdl.handle.net/11449/207396
identifier_str_mv Gene Expression Patterns, v. 40.
1872-7298
1567-133X
10.1016/j.gep.2021.119169
2-s2.0-85102054462
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gene Expression Patterns
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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