Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.gep.2021.119169 http://hdl.handle.net/11449/207396 |
Resumo: | Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males. |
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Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testesanti-Müllerian hormoneCommon carpSpermatogenesisTestisAnti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males.Centers for Disease Control and PreventionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade Estadual PaulistaASCRS Research FoundationAquaculture Program (CAUNESP) São Paulo State University (UNESP), JaboticabalReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), BotucatuFaculty of Veterinary Medicine and Animal Science San Martín University Foundation (FUSM)Department of Cell Biology and Histology Faculty of Biology Universidad de Murcia IMIB-ArrixacaDepartment of Orthopaedics Muscle skeletal Research West Virginia UniversityAquaculture Program (CAUNESP) São Paulo State University (UNESP), JaboticabalReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), BotucatuUniversidade Estadual Paulista (Unesp)San Martín University Foundation (FUSM)IMIB-ArrixacaWest Virginia UniversityOliveira, Marcos A. [UNESP]Martinez, Emanuel R.M. [UNESP]Butzge, Arno J. [UNESP]Doretto, Lucas B. [UNESP]Ricci, Juliana M.B. [UNESP]Rodrigues, Maira S. [UNESP]Vigoya, Angel A.A. [UNESP]Gómez-González, Núria E.Stewart, Amanda B.Nóbrega, Rafael H. [UNESP]2021-06-25T10:54:33Z2021-06-25T10:54:33Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.gep.2021.119169Gene Expression Patterns, v. 40.1872-72981567-133Xhttp://hdl.handle.net/11449/20739610.1016/j.gep.2021.1191692-s2.0-85102054462Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGene Expression Patternsinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/207396Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:26.883971Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
title |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
spellingShingle |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes Oliveira, Marcos A. [UNESP] anti-Müllerian hormone Common carp Spermatogenesis Testis |
title_short |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
title_full |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
title_fullStr |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
title_full_unstemmed |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
title_sort |
Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes |
author |
Oliveira, Marcos A. [UNESP] |
author_facet |
Oliveira, Marcos A. [UNESP] Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Rodrigues, Maira S. [UNESP] Vigoya, Angel A.A. [UNESP] Gómez-González, Núria E. Stewart, Amanda B. Nóbrega, Rafael H. [UNESP] |
author_role |
author |
author2 |
Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Rodrigues, Maira S. [UNESP] Vigoya, Angel A.A. [UNESP] Gómez-González, Núria E. Stewart, Amanda B. Nóbrega, Rafael H. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) San Martín University Foundation (FUSM) IMIB-Arrixaca West Virginia University |
dc.contributor.author.fl_str_mv |
Oliveira, Marcos A. [UNESP] Martinez, Emanuel R.M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Ricci, Juliana M.B. [UNESP] Rodrigues, Maira S. [UNESP] Vigoya, Angel A.A. [UNESP] Gómez-González, Núria E. Stewart, Amanda B. Nóbrega, Rafael H. [UNESP] |
dc.subject.por.fl_str_mv |
anti-Müllerian hormone Common carp Spermatogenesis Testis |
topic |
anti-Müllerian hormone Common carp Spermatogenesis Testis |
description |
Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:54:33Z 2021-06-25T10:54:33Z 2021-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.gep.2021.119169 Gene Expression Patterns, v. 40. 1872-7298 1567-133X http://hdl.handle.net/11449/207396 10.1016/j.gep.2021.119169 2-s2.0-85102054462 |
url |
http://dx.doi.org/10.1016/j.gep.2021.119169 http://hdl.handle.net/11449/207396 |
identifier_str_mv |
Gene Expression Patterns, v. 40. 1872-7298 1567-133X 10.1016/j.gep.2021.119169 2-s2.0-85102054462 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gene Expression Patterns |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129426104254464 |