Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection

Detalhes bibliográficos
Autor(a) principal: Rossoni, Rodnei Dennis [UNESP]
Data de Publicação: 2017
Outros Autores: Fuchs, Beth Burgwyn, De Barros, Patrícia Pimentel [UNESP], Dos Santos Velloso, Marisol [UNESP], Jorge, Antonio Olavo Cardoso [UNESP], Junqueira, Juliana Campos [UNESP], Mylonakis, Eleftherios
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0173332
http://hdl.handle.net/11449/174317
Resumo: Probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. Using the non-vertebrate model host Galleria mellonella, we evaluated whether clinical isolates of Lactobacillus spp. are able to provide protection against Candida albicans infection. Among different strains of Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus fermentum, we verified that L. paracasei 28.4 strain had the greatest ability to prolong the survival of larvae infected with a lethal dose of C. albicans. We found that the injection of 107 cells/larvae of L. paracasei into G. mellonella larvae infected by C. albicans increased the survival of these insects compared to the control group (P = 0.0001). After that, we investigated the immune mechanisms involved in the protection against C. albicans infection, evaluating the number of hemocytes and the gene expression of antifungal peptides. We found that L. paracasei increased the hemocyte quantity (2.38 x 106 cells/mL) in relation to the control group (1.29 x 106 cells/mL), indicating that this strain is capable of raising the number of circulating hemocytes into the G. mellonella hemolymph. Further, we found that L. paracasei 28.4 upregulated genes that encode the antifungal peptides galiomicin and gallerymicin. In relation to the control group, L. paracasei 28.4 increased gene expression of galiomicin by 6.67-fold and 17.29-fold for gallerymicin. Finally, we verified that the prophylactic provision of probiotic led to a significant reduction of the number of fungal cells in G. mellonella hemolymph. In conclusion, L. paracasei 28.4 can modulate the immune system of G. mellonella and protect against candidiasis.
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spelling Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infectionProbiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. Using the non-vertebrate model host Galleria mellonella, we evaluated whether clinical isolates of Lactobacillus spp. are able to provide protection against Candida albicans infection. Among different strains of Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus fermentum, we verified that L. paracasei 28.4 strain had the greatest ability to prolong the survival of larvae infected with a lethal dose of C. albicans. We found that the injection of 107 cells/larvae of L. paracasei into G. mellonella larvae infected by C. albicans increased the survival of these insects compared to the control group (P = 0.0001). After that, we investigated the immune mechanisms involved in the protection against C. albicans infection, evaluating the number of hemocytes and the gene expression of antifungal peptides. We found that L. paracasei increased the hemocyte quantity (2.38 x 106 cells/mL) in relation to the control group (1.29 x 106 cells/mL), indicating that this strain is capable of raising the number of circulating hemocytes into the G. mellonella hemolymph. Further, we found that L. paracasei 28.4 upregulated genes that encode the antifungal peptides galiomicin and gallerymicin. In relation to the control group, L. paracasei 28.4 increased gene expression of galiomicin by 6.67-fold and 17.29-fold for gallerymicin. Finally, we verified that the prophylactic provision of probiotic led to a significant reduction of the number of fungal cells in G. mellonella hemolymph. In conclusion, L. paracasei 28.4 can modulate the immune system of G. mellonella and protect against candidiasis.Department of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP São José dos CamposDivision of Infectious Diseases Alpert Medical School of Brown University Rhode Island HospitalDepartment of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP São José dos CamposUniversidade Estadual Paulista (Unesp)Rhode Island HospitalRossoni, Rodnei Dennis [UNESP]Fuchs, Beth BurgwynDe Barros, Patrícia Pimentel [UNESP]Dos Santos Velloso, Marisol [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]Mylonakis, Eleftherios2018-12-11T17:10:30Z2018-12-11T17:10:30Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0173332PLoS ONE, v. 12, n. 3, 2017.1932-6203http://hdl.handle.net/11449/17431710.1371/journal.pone.01733322-s2.0-850148025152-s2.0-85014802515.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-10-25T06:05:42Zoai:repositorio.unesp.br:11449/174317Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-25T06:05:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
title Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
spellingShingle Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
Rossoni, Rodnei Dennis [UNESP]
title_short Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
title_full Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
title_fullStr Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
title_full_unstemmed Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
title_sort Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection
author Rossoni, Rodnei Dennis [UNESP]
author_facet Rossoni, Rodnei Dennis [UNESP]
Fuchs, Beth Burgwyn
De Barros, Patrícia Pimentel [UNESP]
Dos Santos Velloso, Marisol [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
Mylonakis, Eleftherios
author_role author
author2 Fuchs, Beth Burgwyn
De Barros, Patrícia Pimentel [UNESP]
Dos Santos Velloso, Marisol [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
Mylonakis, Eleftherios
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Rhode Island Hospital
dc.contributor.author.fl_str_mv Rossoni, Rodnei Dennis [UNESP]
Fuchs, Beth Burgwyn
De Barros, Patrícia Pimentel [UNESP]
Dos Santos Velloso, Marisol [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
Mylonakis, Eleftherios
description Probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. Using the non-vertebrate model host Galleria mellonella, we evaluated whether clinical isolates of Lactobacillus spp. are able to provide protection against Candida albicans infection. Among different strains of Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus fermentum, we verified that L. paracasei 28.4 strain had the greatest ability to prolong the survival of larvae infected with a lethal dose of C. albicans. We found that the injection of 107 cells/larvae of L. paracasei into G. mellonella larvae infected by C. albicans increased the survival of these insects compared to the control group (P = 0.0001). After that, we investigated the immune mechanisms involved in the protection against C. albicans infection, evaluating the number of hemocytes and the gene expression of antifungal peptides. We found that L. paracasei increased the hemocyte quantity (2.38 x 106 cells/mL) in relation to the control group (1.29 x 106 cells/mL), indicating that this strain is capable of raising the number of circulating hemocytes into the G. mellonella hemolymph. Further, we found that L. paracasei 28.4 upregulated genes that encode the antifungal peptides galiomicin and gallerymicin. In relation to the control group, L. paracasei 28.4 increased gene expression of galiomicin by 6.67-fold and 17.29-fold for gallerymicin. Finally, we verified that the prophylactic provision of probiotic led to a significant reduction of the number of fungal cells in G. mellonella hemolymph. In conclusion, L. paracasei 28.4 can modulate the immune system of G. mellonella and protect against candidiasis.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
2018-12-11T17:10:30Z
2018-12-11T17:10:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0173332
PLoS ONE, v. 12, n. 3, 2017.
1932-6203
http://hdl.handle.net/11449/174317
10.1371/journal.pone.0173332
2-s2.0-85014802515
2-s2.0-85014802515.pdf
url http://dx.doi.org/10.1371/journal.pone.0173332
http://hdl.handle.net/11449/174317
identifier_str_mv PLoS ONE, v. 12, n. 3, 2017.
1932-6203
10.1371/journal.pone.0173332
2-s2.0-85014802515
2-s2.0-85014802515.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE
1,164
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dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
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collection Repositório Institucional da UNESP
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