Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2017.04.001 http://hdl.handle.net/11449/174421 |
Resumo: | This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis. Spermatic kinetics, integrity and the stability of plasma membrane, damage to the acrosome and fertility following laparoscopic artificial insemination were evaluated in samples collected in an artificial vagina (AV) and from epididymides stored at room temperature for zero (G0), six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours post mortem. There were no significant differences in spermatic parameters between the methods of sample collection, except for progressive motility and velocity according to the straight path(VSL). G48 samples had significant lower total motility(TM), progressive motility(PM), kinetic parameters, viability and acrosomal integrity. Pregnancy rate after insemination was similar for samples collected using AV, and the G0, G6, G12 and G24 samples. In conclusion, ovine epididymides can be exposed to room temperature, for up to 24 h post mortem, with no effect on viability and fertility of cryopreserved seminal samples. The addition of seminal plasma to the cryopreservation extender had no effect on spermatozoa quality nor fertility. |
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Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortemCASAEpididymisSeminal plasmaThis study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis. Spermatic kinetics, integrity and the stability of plasma membrane, damage to the acrosome and fertility following laparoscopic artificial insemination were evaluated in samples collected in an artificial vagina (AV) and from epididymides stored at room temperature for zero (G0), six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours post mortem. There were no significant differences in spermatic parameters between the methods of sample collection, except for progressive motility and velocity according to the straight path(VSL). G48 samples had significant lower total motility(TM), progressive motility(PM), kinetic parameters, viability and acrosomal integrity. Pregnancy rate after insemination was similar for samples collected using AV, and the G0, G6, G12 and G24 samples. In conclusion, ovine epididymides can be exposed to room temperature, for up to 24 h post mortem, with no effect on viability and fertility of cryopreserved seminal samples. The addition of seminal plasma to the cryopreservation extender had no effect on spermatozoa quality nor fertility.Department of Technology Postgraduate Studies in Bioprocess Engineering and Biotechnology Human and Animal Health Federal University of Paraná, Rua dos Funcionários, 1540Department of Veterinary Medicine Postgraduate Studies in Animal Science Pontifical Catholic University of Paraná, Rua Imaculada Conceição, 1155, Prado VelhoDepartment of Animal Reproduction and Veterinary Radiology- FMVZ – UNESP, Distrito de Rubião Júnior, S/nDepartment of Animal Reproduction and Veterinary Radiology- FMVZ – UNESP, Distrito de Rubião Júnior, S/nFederal University of ParanáPontifical Catholic University of ParanáUniversidade Estadual Paulista (Unesp)Bergstein-Galan, Tácia GomesWeiss, Romildo RomualdoBertol, Melina Andrea F.Abreu, Ana Cláudia M.R.Busato, EduardaKozicki, Luiz E.Bicudo, Sony Dimas [UNESP]2018-12-11T17:11:02Z2018-12-11T17:11:02Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article69-75application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2017.04.001Theriogenology, v. 96, p. 69-75.0093-691Xhttp://hdl.handle.net/11449/17442110.1016/j.theriogenology.2017.04.0012-s2.0-850169360342-s2.0-85016936034.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:01:31Zoai:repositorio.unesp.br:11449/174421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
title |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
spellingShingle |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem Bergstein-Galan, Tácia Gomes CASA Epididymis Seminal plasma |
title_short |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
title_full |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
title_fullStr |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
title_full_unstemmed |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
title_sort |
Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem |
author |
Bergstein-Galan, Tácia Gomes |
author_facet |
Bergstein-Galan, Tácia Gomes Weiss, Romildo Romualdo Bertol, Melina Andrea F. Abreu, Ana Cláudia M.R. Busato, Eduarda Kozicki, Luiz E. Bicudo, Sony Dimas [UNESP] |
author_role |
author |
author2 |
Weiss, Romildo Romualdo Bertol, Melina Andrea F. Abreu, Ana Cláudia M.R. Busato, Eduarda Kozicki, Luiz E. Bicudo, Sony Dimas [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Paraná Pontifical Catholic University of Paraná Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bergstein-Galan, Tácia Gomes Weiss, Romildo Romualdo Bertol, Melina Andrea F. Abreu, Ana Cláudia M.R. Busato, Eduarda Kozicki, Luiz E. Bicudo, Sony Dimas [UNESP] |
dc.subject.por.fl_str_mv |
CASA Epididymis Seminal plasma |
topic |
CASA Epididymis Seminal plasma |
description |
This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis. Spermatic kinetics, integrity and the stability of plasma membrane, damage to the acrosome and fertility following laparoscopic artificial insemination were evaluated in samples collected in an artificial vagina (AV) and from epididymides stored at room temperature for zero (G0), six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours post mortem. There were no significant differences in spermatic parameters between the methods of sample collection, except for progressive motility and velocity according to the straight path(VSL). G48 samples had significant lower total motility(TM), progressive motility(PM), kinetic parameters, viability and acrosomal integrity. Pregnancy rate after insemination was similar for samples collected using AV, and the G0, G6, G12 and G24 samples. In conclusion, ovine epididymides can be exposed to room temperature, for up to 24 h post mortem, with no effect on viability and fertility of cryopreserved seminal samples. The addition of seminal plasma to the cryopreservation extender had no effect on spermatozoa quality nor fertility. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:11:02Z 2018-12-11T17:11:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2017.04.001 Theriogenology, v. 96, p. 69-75. 0093-691X http://hdl.handle.net/11449/174421 10.1016/j.theriogenology.2017.04.001 2-s2.0-85016936034 2-s2.0-85016936034.pdf |
url |
http://dx.doi.org/10.1016/j.theriogenology.2017.04.001 http://hdl.handle.net/11449/174421 |
identifier_str_mv |
Theriogenology, v. 96, p. 69-75. 0093-691X 10.1016/j.theriogenology.2017.04.001 2-s2.0-85016936034 2-s2.0-85016936034.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
69-75 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021331594379264 |