Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol7-2gmr396 http://hdl.handle.net/11449/70495 |
Resumo: | Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT until complete cytogenetic remission (CCR). We investigated the disease-free survival, overall survival in 3 years and transplant-related mortality rates, too. Bone marrow samples were collected at 1, 2, 3, 4, 6, 12, and 24 months after transplantation and additional intervals as necessary. Success rate of the FISH analyses was 100%. CCR was achieved in 75% of the patients, within on average of 3.9 months; 45% patients showed CCR within 60 days after HSCT. After 3 years of the allo-HSCT, overall survival rate was 60%, disease-free survival was 50% and the transplant-related mortality rate was 40%. The study demonstrated that the BCR-ABL FISH assay is useful for follow-up of chronic myeloid leukemia patients after HSCT and that the clinical outcome parameters in our patient cohort were similar to those described for other bone marrow transplantation units. ©FUNPEC-RP. |
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Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISHBCR-ABL fusionChronic myelogenous leukemiaCytogenetic remissionDisease-free survivalFluorescence in situ hybridizationStem cell transplantationBCR ABL proteinadolescentadultallogeneic hematopoietic stem cell transplantationbone marrowBrazilcancer mortalitycancer regressionchronic myeloid leukemiaclinical articledisease free survivalfluorescence in situ hybridizationgene rearrangementhospital departmenthumanhuman tissueoverall survivalsurvival ratesurvival timeAdolescentAdultBone Marrow TransplantationDisease-Free SurvivalHematopoietic Stem Cell TransplantationHospital DepartmentsHumansIn Situ Hybridization, FluorescenceLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMiddle AgedPrognosisSurvival RateTransplantation, HomologousTreatment OutcomeInvestigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT until complete cytogenetic remission (CCR). We investigated the disease-free survival, overall survival in 3 years and transplant-related mortality rates, too. Bone marrow samples were collected at 1, 2, 3, 4, 6, 12, and 24 months after transplantation and additional intervals as necessary. Success rate of the FISH analyses was 100%. CCR was achieved in 75% of the patients, within on average of 3.9 months; 45% patients showed CCR within 60 days after HSCT. After 3 years of the allo-HSCT, overall survival rate was 60%, disease-free survival was 50% and the transplant-related mortality rate was 40%. The study demonstrated that the BCR-ABL FISH assay is useful for follow-up of chronic myeloid leukemia patients after HSCT and that the clinical outcome parameters in our patient cohort were similar to those described for other bone marrow transplantation units. ©FUNPEC-RP.Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista, São José do Rio Preto, SPHemocentro Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, SPUnidade de Transplante de Medula Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, SPUniversity of Colorado Comprehensive Cancer Center, Denver, CODepartamento de Biologia Molecular Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SPDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista, São José do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Fundação Faculdade Regional de Medicina de São José do Rio PretoUniversity of Colorado Comprehensive Cancer CenterFaculdade de Medicina de São José do Rio Preto (FAMERP)Vendrame-Goloni, C. B. [UNESP]Carvalho-Salles, A. B.Ruiz, M. A.Ricci, O.Varella-Garcia, M.Fett-Conte, A. C.2014-05-27T11:23:37Z2014-05-27T11:23:37Z2008-07-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article417-423application/pdfhttp://dx.doi.org/10.4238/vol7-2gmr396Genetics and Molecular Research, v. 7, n. 2, p. 417-423, 2008.1676-5680http://hdl.handle.net/11449/7049510.4238/vol7-2gmr3962-s2.0-479491111832-s2.0-47949111183.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-01-20T06:37:23Zoai:repositorio.unesp.br:11449/70495Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:32:32.632362Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
title |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
spellingShingle |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH Vendrame-Goloni, C. B. [UNESP] BCR-ABL fusion Chronic myelogenous leukemia Cytogenetic remission Disease-free survival Fluorescence in situ hybridization Stem cell transplantation BCR ABL protein adolescent adult allogeneic hematopoietic stem cell transplantation bone marrow Brazil cancer mortality cancer regression chronic myeloid leukemia clinical article disease free survival fluorescence in situ hybridization gene rearrangement hospital department human human tissue overall survival survival rate survival time Adolescent Adult Bone Marrow Transplantation Disease-Free Survival Hematopoietic Stem Cell Transplantation Hospital Departments Humans In Situ Hybridization, Fluorescence Leukemia, Myelogenous, Chronic, BCR-ABL Positive Middle Aged Prognosis Survival Rate Transplantation, Homologous Treatment Outcome |
title_short |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
title_full |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
title_fullStr |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
title_full_unstemmed |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
title_sort |
Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: The experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH |
author |
Vendrame-Goloni, C. B. [UNESP] |
author_facet |
Vendrame-Goloni, C. B. [UNESP] Carvalho-Salles, A. B. Ruiz, M. A. Ricci, O. Varella-Garcia, M. Fett-Conte, A. C. |
author_role |
author |
author2 |
Carvalho-Salles, A. B. Ruiz, M. A. Ricci, O. Varella-Garcia, M. Fett-Conte, A. C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fundação Faculdade Regional de Medicina de São José do Rio Preto University of Colorado Comprehensive Cancer Center Faculdade de Medicina de São José do Rio Preto (FAMERP) |
dc.contributor.author.fl_str_mv |
Vendrame-Goloni, C. B. [UNESP] Carvalho-Salles, A. B. Ruiz, M. A. Ricci, O. Varella-Garcia, M. Fett-Conte, A. C. |
dc.subject.por.fl_str_mv |
BCR-ABL fusion Chronic myelogenous leukemia Cytogenetic remission Disease-free survival Fluorescence in situ hybridization Stem cell transplantation BCR ABL protein adolescent adult allogeneic hematopoietic stem cell transplantation bone marrow Brazil cancer mortality cancer regression chronic myeloid leukemia clinical article disease free survival fluorescence in situ hybridization gene rearrangement hospital department human human tissue overall survival survival rate survival time Adolescent Adult Bone Marrow Transplantation Disease-Free Survival Hematopoietic Stem Cell Transplantation Hospital Departments Humans In Situ Hybridization, Fluorescence Leukemia, Myelogenous, Chronic, BCR-ABL Positive Middle Aged Prognosis Survival Rate Transplantation, Homologous Treatment Outcome |
topic |
BCR-ABL fusion Chronic myelogenous leukemia Cytogenetic remission Disease-free survival Fluorescence in situ hybridization Stem cell transplantation BCR ABL protein adolescent adult allogeneic hematopoietic stem cell transplantation bone marrow Brazil cancer mortality cancer regression chronic myeloid leukemia clinical article disease free survival fluorescence in situ hybridization gene rearrangement hospital department human human tissue overall survival survival rate survival time Adolescent Adult Bone Marrow Transplantation Disease-Free Survival Hematopoietic Stem Cell Transplantation Hospital Departments Humans In Situ Hybridization, Fluorescence Leukemia, Myelogenous, Chronic, BCR-ABL Positive Middle Aged Prognosis Survival Rate Transplantation, Homologous Treatment Outcome |
description |
Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT until complete cytogenetic remission (CCR). We investigated the disease-free survival, overall survival in 3 years and transplant-related mortality rates, too. Bone marrow samples were collected at 1, 2, 3, 4, 6, 12, and 24 months after transplantation and additional intervals as necessary. Success rate of the FISH analyses was 100%. CCR was achieved in 75% of the patients, within on average of 3.9 months; 45% patients showed CCR within 60 days after HSCT. After 3 years of the allo-HSCT, overall survival rate was 60%, disease-free survival was 50% and the transplant-related mortality rate was 40%. The study demonstrated that the BCR-ABL FISH assay is useful for follow-up of chronic myeloid leukemia patients after HSCT and that the clinical outcome parameters in our patient cohort were similar to those described for other bone marrow transplantation units. ©FUNPEC-RP. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07-30 2014-05-27T11:23:37Z 2014-05-27T11:23:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol7-2gmr396 Genetics and Molecular Research, v. 7, n. 2, p. 417-423, 2008. 1676-5680 http://hdl.handle.net/11449/70495 10.4238/vol7-2gmr396 2-s2.0-47949111183 2-s2.0-47949111183.pdf |
url |
http://dx.doi.org/10.4238/vol7-2gmr396 http://hdl.handle.net/11449/70495 |
identifier_str_mv |
Genetics and Molecular Research, v. 7, n. 2, p. 417-423, 2008. 1676-5680 10.4238/vol7-2gmr396 2-s2.0-47949111183 2-s2.0-47949111183.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
417-423 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129530002407424 |