Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.acthis.2017.07.003 http://hdl.handle.net/11449/174924 |
Resumo: | Objectives Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. Materials and Methods Critical-size defects of 7 mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty. The animals were euthanized at two different time points (30 and 60 days post-operation). All defects were recovered with a absorbable membrane of bovine cortical bone. Histological, histometric, and immunohistochemical (osteocalcin) assessments were carried out at 30 and 60 days post-operation. Results At 30 days post-operation, all groups showed areas of bone formation, predominantly when autogenous grafts were used. However, there were no statistically significant differences between the treatment groups (p > 0.05). After 60 days, there were similarities in the bone formation patterns between the β-TCP (26.32±) and Bio-Oss (17.35±) groups (p = 0.549). In terms of the immunohistochemical assessment of osteocalcin, the clot group showed light to moderate staining at 30 and 60 days. The autogenous group showed moderate staining at 30 days and moderate to intense staining after 60 days. The Bio-Oss group showed light to moderate staining after 30 days and intense staining at 60 days. The β-TCP group showed moderate staining at 30 and 60 days post-operation. Conclusion β-TCP is a good osteoconductive material with similar effects to those of inorganic bovine bone graft and is suitable for utilization in the repair of bone defects. |
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Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP)Animal experimentsBiomaterialsBone substitutesObjectives Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. Materials and Methods Critical-size defects of 7 mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty. The animals were euthanized at two different time points (30 and 60 days post-operation). All defects were recovered with a absorbable membrane of bovine cortical bone. Histological, histometric, and immunohistochemical (osteocalcin) assessments were carried out at 30 and 60 days post-operation. Results At 30 days post-operation, all groups showed areas of bone formation, predominantly when autogenous grafts were used. However, there were no statistically significant differences between the treatment groups (p > 0.05). After 60 days, there were similarities in the bone formation patterns between the β-TCP (26.32±) and Bio-Oss (17.35±) groups (p = 0.549). In terms of the immunohistochemical assessment of osteocalcin, the clot group showed light to moderate staining at 30 and 60 days. The autogenous group showed moderate staining at 30 days and moderate to intense staining after 60 days. The Bio-Oss group showed light to moderate staining after 30 days and intense staining at 60 days. The β-TCP group showed moderate staining at 30 and 60 days post-operation. Conclusion β-TCP is a good osteoconductive material with similar effects to those of inorganic bovine bone graft and is suitable for utilization in the repair of bone defects.DDS Araçatuba Dental School – UNESPDDS MSc student on Dental Implants from São Leopoldo Mandic Dental SchoolAdjunct Professor from Department of Surgery and Integrated clinic Araçatuba Dental School – UNESPProfessor of Oral and Maxillofacial Surgery Department from Bauru Dental School – USPAssistant Doctor Professor from Department of Surgery and Integrated clinic Araçatuba Dental School – UNESPDDS Araçatuba Dental School – UNESPAdjunct Professor from Department of Surgery and Integrated clinic Araçatuba Dental School – UNESPAssistant Doctor Professor from Department of Surgery and Integrated clinic Araçatuba Dental School – UNESPUniversidade Estadual Paulista (Unesp)MSc student on Dental Implants from São Leopoldo Mandic Dental SchoolUniversidade de São Paulo (USP)de Freitas Silva, Leonardo [UNESP]de Carvalho Reis, Erik Neiva Ribeiro [UNESP]Barbara, Tânia AparecidaBonardi, João Paulo [UNESP]Garcia, Idelmo Rangel [UNESP]de Carvalho, Paulo Sérgio PerriPonzoni, Daniela [UNESP]2018-12-11T17:13:28Z2018-12-11T17:13:28Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article624-631application/pdfhttp://dx.doi.org/10.1016/j.acthis.2017.07.003Acta Histochemica, v. 119, n. 6, p. 624-631, 2017.1618-03720065-1281http://hdl.handle.net/11449/17492410.1016/j.acthis.2017.07.0032-s2.0-850245052542-s2.0-85024505254.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Histochemica0,661info:eu-repo/semantics/openAccess2023-10-30T06:10:49Zoai:repositorio.unesp.br:11449/174924Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-30T06:10:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
title |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
spellingShingle |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) de Freitas Silva, Leonardo [UNESP] Animal experiments Biomaterials Bone substitutes |
title_short |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
title_full |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
title_fullStr |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
title_full_unstemmed |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
title_sort |
Assessment of bone repair in critical-size defect in the calvarium of rats after the implantation of tricalcium phosphate beta (β-TCP) |
author |
de Freitas Silva, Leonardo [UNESP] |
author_facet |
de Freitas Silva, Leonardo [UNESP] de Carvalho Reis, Erik Neiva Ribeiro [UNESP] Barbara, Tânia Aparecida Bonardi, João Paulo [UNESP] Garcia, Idelmo Rangel [UNESP] de Carvalho, Paulo Sérgio Perri Ponzoni, Daniela [UNESP] |
author_role |
author |
author2 |
de Carvalho Reis, Erik Neiva Ribeiro [UNESP] Barbara, Tânia Aparecida Bonardi, João Paulo [UNESP] Garcia, Idelmo Rangel [UNESP] de Carvalho, Paulo Sérgio Perri Ponzoni, Daniela [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) MSc student on Dental Implants from São Leopoldo Mandic Dental School Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Freitas Silva, Leonardo [UNESP] de Carvalho Reis, Erik Neiva Ribeiro [UNESP] Barbara, Tânia Aparecida Bonardi, João Paulo [UNESP] Garcia, Idelmo Rangel [UNESP] de Carvalho, Paulo Sérgio Perri Ponzoni, Daniela [UNESP] |
dc.subject.por.fl_str_mv |
Animal experiments Biomaterials Bone substitutes |
topic |
Animal experiments Biomaterials Bone substitutes |
description |
Objectives Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. Materials and Methods Critical-size defects of 7 mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty. The animals were euthanized at two different time points (30 and 60 days post-operation). All defects were recovered with a absorbable membrane of bovine cortical bone. Histological, histometric, and immunohistochemical (osteocalcin) assessments were carried out at 30 and 60 days post-operation. Results At 30 days post-operation, all groups showed areas of bone formation, predominantly when autogenous grafts were used. However, there were no statistically significant differences between the treatment groups (p > 0.05). After 60 days, there were similarities in the bone formation patterns between the β-TCP (26.32±) and Bio-Oss (17.35±) groups (p = 0.549). In terms of the immunohistochemical assessment of osteocalcin, the clot group showed light to moderate staining at 30 and 60 days. The autogenous group showed moderate staining at 30 days and moderate to intense staining after 60 days. The Bio-Oss group showed light to moderate staining after 30 days and intense staining at 60 days. The β-TCP group showed moderate staining at 30 and 60 days post-operation. Conclusion β-TCP is a good osteoconductive material with similar effects to those of inorganic bovine bone graft and is suitable for utilization in the repair of bone defects. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:13:28Z 2018-12-11T17:13:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.acthis.2017.07.003 Acta Histochemica, v. 119, n. 6, p. 624-631, 2017. 1618-0372 0065-1281 http://hdl.handle.net/11449/174924 10.1016/j.acthis.2017.07.003 2-s2.0-85024505254 2-s2.0-85024505254.pdf |
url |
http://dx.doi.org/10.1016/j.acthis.2017.07.003 http://hdl.handle.net/11449/174924 |
identifier_str_mv |
Acta Histochemica, v. 119, n. 6, p. 624-631, 2017. 1618-0372 0065-1281 10.1016/j.acthis.2017.07.003 2-s2.0-85024505254 2-s2.0-85024505254.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Histochemica 0,661 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
624-631 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964758887104512 |