Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms24076104 http://hdl.handle.net/11449/248713 |
Resumo: | Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence. |
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Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish ModelCOVID-19cytokine stormsoxidative stressphotobiomodulationrSpikezebrafishAlthough the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Orthodontics São Leopoldo Mandic CollegeInstituto de Química de São Carlos Universidade de São PauloThe National Institute of Science and Technology in Bioanalyses INCTBioInstitute of Biomedical Sciences University of São Paulo (USP)Oswaldo Cruz Foundation (Fiocruz)Laboratório de Fisiologia de Peixes Programa de Pós-Graduação em Bioexperimentação Escola de Ciências Agrárias Inovação e Negócios Universidade de Passo FundoInstitute of Biomedical Sciences Federal University Minas GeraisDepartment of Genomics Faculty of Biosciences and Aquaculture Nord UniversityBiological Sciences Special Academic Unit Federal University of JataiKeizo Asami Institute Federal University of PernambucoSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Institute of Motricity Sciences Department of Physical Therapy Federal University of AlfenasDepartamento de Ciências Biomoleculares Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São PauloIntegrated Structural Biology Platform Carlos Chagas Institute FIOCRUZ ParanáCenter of Innovation and Development Laboratory of Development and Innovation Butantan InstituteFaculty of Medicine University of São Paulo (USP)Laboratory of Genetic and Sanitary Control Technical Board of Support for Teaching and Research Faculty of Medicine University of Sao PauloDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Laboratory of Toxicology Applied to the Environment Goiano Federal Institute, Urutaí CampusDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)CAPES: 0459/20CAPES: 110CAPES: 465389/2014-7CAPES: 88887.504531/2020-00CAPES: Project CG 19Universidade Estadual Paulista (UNESP)São Leopoldo Mandic CollegeUniversidade de São Paulo (USP)INCTBioOswaldo Cruz Foundation (Fiocruz)Universidade de Passo FundoFederal University Minas GeraisNord UniversityFederal University of JataiUniversidade Federal de Pernambuco (UFPE)Federal University of AlfenasFIOCRUZ ParanáLaboratory of Development and Innovation Butantan InstituteGoiano Federal InstituteRosa, Ivana F. [UNESP]Peçanha, Ana P. B.Carvalho, Tábata R. B.Alexandre, Leonardo S.Ferreira, Vinícius G.Doretto, Lucas B. [UNESP]Souza, Beatriz M. [UNESP]Nakajima, Rafael T. [UNESP]da Silva, PatrickBarbosa, Ana P.Gomes-de-Pontes, LeticiaBomfim, Camila G.Machado-Santelli, Glaucia M.Condino-Neto, AntonioGuzzo, Cristiane R.Peron, Jean P. S.Andrade-Silva, MagaiverCâmara, Niels O. S.Garnique, Anali M. B.Medeiros, Renata J.Ferraris, Fausto K.Barcellos, Leonardo J. G.Correia-Junior, Jose D.Galindo-Villegas, JorgeMachado, Mônica F. R.Castoldi, AngelaOliveira, Susana L. [UNESP]Costa, Camila C. [UNESP]Belo, Marco A. A. [UNESP]Galdino, GiovaneSgro, Germán G.Bueno, Natalia F.Eto, Silas F.Veras, Flávio P.Fernandes, Bianca H. V.Sanches, Paulo R. S. [UNESP]Cilli, Eduardo M. [UNESP]Malafaia, GuilhermeNóbrega, Rafael H. [UNESP]Garcez, Aguinaldo S.Carrilho, EmanuelCharlie-Silva, Ives [UNESP]2023-07-29T13:51:36Z2023-07-29T13:51:36Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24076104International Journal of Molecular Sciences, v. 24, n. 7, 2023.1422-00671661-6596http://hdl.handle.net/11449/24871310.3390/ijms240761042-s2.0-85152799400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:51:36Zoai:repositorio.unesp.br:11449/248713Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:10.639353Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
title |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
spellingShingle |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model Rosa, Ivana F. [UNESP] COVID-19 cytokine storms oxidative stress photobiomodulation rSpike zebrafish |
title_short |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
title_full |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
title_fullStr |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
title_full_unstemmed |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
title_sort |
Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model |
author |
Rosa, Ivana F. [UNESP] |
author_facet |
Rosa, Ivana F. [UNESP] Peçanha, Ana P. B. Carvalho, Tábata R. B. Alexandre, Leonardo S. Ferreira, Vinícius G. Doretto, Lucas B. [UNESP] Souza, Beatriz M. [UNESP] Nakajima, Rafael T. [UNESP] da Silva, Patrick Barbosa, Ana P. Gomes-de-Pontes, Leticia Bomfim, Camila G. Machado-Santelli, Glaucia M. Condino-Neto, Antonio Guzzo, Cristiane R. Peron, Jean P. S. Andrade-Silva, Magaiver Câmara, Niels O. S. Garnique, Anali M. B. Medeiros, Renata J. Ferraris, Fausto K. Barcellos, Leonardo J. G. Correia-Junior, Jose D. Galindo-Villegas, Jorge Machado, Mônica F. R. Castoldi, Angela Oliveira, Susana L. [UNESP] Costa, Camila C. [UNESP] Belo, Marco A. A. [UNESP] Galdino, Giovane Sgro, Germán G. Bueno, Natalia F. Eto, Silas F. Veras, Flávio P. Fernandes, Bianca H. V. Sanches, Paulo R. S. [UNESP] Cilli, Eduardo M. [UNESP] Malafaia, Guilherme Nóbrega, Rafael H. [UNESP] Garcez, Aguinaldo S. Carrilho, Emanuel Charlie-Silva, Ives [UNESP] |
author_role |
author |
author2 |
Peçanha, Ana P. B. Carvalho, Tábata R. B. Alexandre, Leonardo S. Ferreira, Vinícius G. Doretto, Lucas B. [UNESP] Souza, Beatriz M. [UNESP] Nakajima, Rafael T. [UNESP] da Silva, Patrick Barbosa, Ana P. Gomes-de-Pontes, Leticia Bomfim, Camila G. Machado-Santelli, Glaucia M. Condino-Neto, Antonio Guzzo, Cristiane R. Peron, Jean P. S. Andrade-Silva, Magaiver Câmara, Niels O. S. Garnique, Anali M. B. Medeiros, Renata J. Ferraris, Fausto K. Barcellos, Leonardo J. G. Correia-Junior, Jose D. Galindo-Villegas, Jorge Machado, Mônica F. R. Castoldi, Angela Oliveira, Susana L. [UNESP] Costa, Camila C. [UNESP] Belo, Marco A. A. [UNESP] Galdino, Giovane Sgro, Germán G. Bueno, Natalia F. Eto, Silas F. Veras, Flávio P. Fernandes, Bianca H. V. Sanches, Paulo R. S. [UNESP] Cilli, Eduardo M. [UNESP] Malafaia, Guilherme Nóbrega, Rafael H. [UNESP] Garcez, Aguinaldo S. Carrilho, Emanuel Charlie-Silva, Ives [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) São Leopoldo Mandic College Universidade de São Paulo (USP) INCTBio Oswaldo Cruz Foundation (Fiocruz) Universidade de Passo Fundo Federal University Minas Gerais Nord University Federal University of Jatai Universidade Federal de Pernambuco (UFPE) Federal University of Alfenas FIOCRUZ Paraná Laboratory of Development and Innovation Butantan Institute Goiano Federal Institute |
dc.contributor.author.fl_str_mv |
Rosa, Ivana F. [UNESP] Peçanha, Ana P. B. Carvalho, Tábata R. B. Alexandre, Leonardo S. Ferreira, Vinícius G. Doretto, Lucas B. [UNESP] Souza, Beatriz M. [UNESP] Nakajima, Rafael T. [UNESP] da Silva, Patrick Barbosa, Ana P. Gomes-de-Pontes, Leticia Bomfim, Camila G. Machado-Santelli, Glaucia M. Condino-Neto, Antonio Guzzo, Cristiane R. Peron, Jean P. S. Andrade-Silva, Magaiver Câmara, Niels O. S. Garnique, Anali M. B. Medeiros, Renata J. Ferraris, Fausto K. Barcellos, Leonardo J. G. Correia-Junior, Jose D. Galindo-Villegas, Jorge Machado, Mônica F. R. Castoldi, Angela Oliveira, Susana L. [UNESP] Costa, Camila C. [UNESP] Belo, Marco A. A. [UNESP] Galdino, Giovane Sgro, Germán G. Bueno, Natalia F. Eto, Silas F. Veras, Flávio P. Fernandes, Bianca H. V. Sanches, Paulo R. S. [UNESP] Cilli, Eduardo M. [UNESP] Malafaia, Guilherme Nóbrega, Rafael H. [UNESP] Garcez, Aguinaldo S. Carrilho, Emanuel Charlie-Silva, Ives [UNESP] |
dc.subject.por.fl_str_mv |
COVID-19 cytokine storms oxidative stress photobiomodulation rSpike zebrafish |
topic |
COVID-19 cytokine storms oxidative stress photobiomodulation rSpike zebrafish |
description |
Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:51:36Z 2023-07-29T13:51:36Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms24076104 International Journal of Molecular Sciences, v. 24, n. 7, 2023. 1422-0067 1661-6596 http://hdl.handle.net/11449/248713 10.3390/ijms24076104 2-s2.0-85152799400 |
url |
http://dx.doi.org/10.3390/ijms24076104 http://hdl.handle.net/11449/248713 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 24, n. 7, 2023. 1422-0067 1661-6596 10.3390/ijms24076104 2-s2.0-85152799400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129538851340288 |