Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model

Detalhes bibliográficos
Autor(a) principal: Rosa, Ivana F. [UNESP]
Data de Publicação: 2023
Outros Autores: Peçanha, Ana P. B., Carvalho, Tábata R. B., Alexandre, Leonardo S., Ferreira, Vinícius G., Doretto, Lucas B. [UNESP], Souza, Beatriz M. [UNESP], Nakajima, Rafael T. [UNESP], da Silva, Patrick, Barbosa, Ana P., Gomes-de-Pontes, Leticia, Bomfim, Camila G., Machado-Santelli, Glaucia M., Condino-Neto, Antonio, Guzzo, Cristiane R., Peron, Jean P. S., Andrade-Silva, Magaiver, Câmara, Niels O. S., Garnique, Anali M. B., Medeiros, Renata J., Ferraris, Fausto K., Barcellos, Leonardo J. G., Correia-Junior, Jose D., Galindo-Villegas, Jorge, Machado, Mônica F. R., Castoldi, Angela, Oliveira, Susana L. [UNESP], Costa, Camila C. [UNESP], Belo, Marco A. A. [UNESP], Galdino, Giovane, Sgro, Germán G., Bueno, Natalia F., Eto, Silas F., Veras, Flávio P., Fernandes, Bianca H. V., Sanches, Paulo R. S. [UNESP], Cilli, Eduardo M. [UNESP], Malafaia, Guilherme, Nóbrega, Rafael H. [UNESP], Garcez, Aguinaldo S., Carrilho, Emanuel, Charlie-Silva, Ives [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ijms24076104
http://hdl.handle.net/11449/248713
Resumo: Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.
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spelling Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish ModelCOVID-19cytokine stormsoxidative stressphotobiomodulationrSpikezebrafishAlthough the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Orthodontics São Leopoldo Mandic CollegeInstituto de Química de São Carlos Universidade de São PauloThe National Institute of Science and Technology in Bioanalyses INCTBioInstitute of Biomedical Sciences University of São Paulo (USP)Oswaldo Cruz Foundation (Fiocruz)Laboratório de Fisiologia de Peixes Programa de Pós-Graduação em Bioexperimentação Escola de Ciências Agrárias Inovação e Negócios Universidade de Passo FundoInstitute of Biomedical Sciences Federal University Minas GeraisDepartment of Genomics Faculty of Biosciences and Aquaculture Nord UniversityBiological Sciences Special Academic Unit Federal University of JataiKeizo Asami Institute Federal University of PernambucoSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Institute of Motricity Sciences Department of Physical Therapy Federal University of AlfenasDepartamento de Ciências Biomoleculares Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São PauloIntegrated Structural Biology Platform Carlos Chagas Institute FIOCRUZ ParanáCenter of Innovation and Development Laboratory of Development and Innovation Butantan InstituteFaculty of Medicine University of São Paulo (USP)Laboratory of Genetic and Sanitary Control Technical Board of Support for Teaching and Research Faculty of Medicine University of Sao PauloDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Laboratory of Toxicology Applied to the Environment Goiano Federal Institute, Urutaí CampusDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)CAPES: 0459/20CAPES: 110CAPES: 465389/2014-7CAPES: 88887.504531/2020-00CAPES: Project CG 19Universidade Estadual Paulista (UNESP)São Leopoldo Mandic CollegeUniversidade de São Paulo (USP)INCTBioOswaldo Cruz Foundation (Fiocruz)Universidade de Passo FundoFederal University Minas GeraisNord UniversityFederal University of JataiUniversidade Federal de Pernambuco (UFPE)Federal University of AlfenasFIOCRUZ ParanáLaboratory of Development and Innovation Butantan InstituteGoiano Federal InstituteRosa, Ivana F. [UNESP]Peçanha, Ana P. B.Carvalho, Tábata R. B.Alexandre, Leonardo S.Ferreira, Vinícius G.Doretto, Lucas B. [UNESP]Souza, Beatriz M. [UNESP]Nakajima, Rafael T. [UNESP]da Silva, PatrickBarbosa, Ana P.Gomes-de-Pontes, LeticiaBomfim, Camila G.Machado-Santelli, Glaucia M.Condino-Neto, AntonioGuzzo, Cristiane R.Peron, Jean P. S.Andrade-Silva, MagaiverCâmara, Niels O. S.Garnique, Anali M. B.Medeiros, Renata J.Ferraris, Fausto K.Barcellos, Leonardo J. G.Correia-Junior, Jose D.Galindo-Villegas, JorgeMachado, Mônica F. R.Castoldi, AngelaOliveira, Susana L. [UNESP]Costa, Camila C. [UNESP]Belo, Marco A. A. [UNESP]Galdino, GiovaneSgro, Germán G.Bueno, Natalia F.Eto, Silas F.Veras, Flávio P.Fernandes, Bianca H. V.Sanches, Paulo R. S. [UNESP]Cilli, Eduardo M. [UNESP]Malafaia, GuilhermeNóbrega, Rafael H. [UNESP]Garcez, Aguinaldo S.Carrilho, EmanuelCharlie-Silva, Ives [UNESP]2023-07-29T13:51:36Z2023-07-29T13:51:36Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24076104International Journal of Molecular Sciences, v. 24, n. 7, 2023.1422-00671661-6596http://hdl.handle.net/11449/24871310.3390/ijms240761042-s2.0-85152799400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:51:36Zoai:repositorio.unesp.br:11449/248713Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:10.639353Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
title Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
spellingShingle Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
Rosa, Ivana F. [UNESP]
COVID-19
cytokine storms
oxidative stress
photobiomodulation
rSpike
zebrafish
title_short Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
title_full Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
title_fullStr Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
title_full_unstemmed Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
title_sort Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
author Rosa, Ivana F. [UNESP]
author_facet Rosa, Ivana F. [UNESP]
Peçanha, Ana P. B.
Carvalho, Tábata R. B.
Alexandre, Leonardo S.
Ferreira, Vinícius G.
Doretto, Lucas B. [UNESP]
Souza, Beatriz M. [UNESP]
Nakajima, Rafael T. [UNESP]
da Silva, Patrick
Barbosa, Ana P.
Gomes-de-Pontes, Leticia
Bomfim, Camila G.
Machado-Santelli, Glaucia M.
Condino-Neto, Antonio
Guzzo, Cristiane R.
Peron, Jean P. S.
Andrade-Silva, Magaiver
Câmara, Niels O. S.
Garnique, Anali M. B.
Medeiros, Renata J.
Ferraris, Fausto K.
Barcellos, Leonardo J. G.
Correia-Junior, Jose D.
Galindo-Villegas, Jorge
Machado, Mônica F. R.
Castoldi, Angela
Oliveira, Susana L. [UNESP]
Costa, Camila C. [UNESP]
Belo, Marco A. A. [UNESP]
Galdino, Giovane
Sgro, Germán G.
Bueno, Natalia F.
Eto, Silas F.
Veras, Flávio P.
Fernandes, Bianca H. V.
Sanches, Paulo R. S. [UNESP]
Cilli, Eduardo M. [UNESP]
Malafaia, Guilherme
Nóbrega, Rafael H. [UNESP]
Garcez, Aguinaldo S.
Carrilho, Emanuel
Charlie-Silva, Ives [UNESP]
author_role author
author2 Peçanha, Ana P. B.
Carvalho, Tábata R. B.
Alexandre, Leonardo S.
Ferreira, Vinícius G.
Doretto, Lucas B. [UNESP]
Souza, Beatriz M. [UNESP]
Nakajima, Rafael T. [UNESP]
da Silva, Patrick
Barbosa, Ana P.
Gomes-de-Pontes, Leticia
Bomfim, Camila G.
Machado-Santelli, Glaucia M.
Condino-Neto, Antonio
Guzzo, Cristiane R.
Peron, Jean P. S.
Andrade-Silva, Magaiver
Câmara, Niels O. S.
Garnique, Anali M. B.
Medeiros, Renata J.
Ferraris, Fausto K.
Barcellos, Leonardo J. G.
Correia-Junior, Jose D.
Galindo-Villegas, Jorge
Machado, Mônica F. R.
Castoldi, Angela
Oliveira, Susana L. [UNESP]
Costa, Camila C. [UNESP]
Belo, Marco A. A. [UNESP]
Galdino, Giovane
Sgro, Germán G.
Bueno, Natalia F.
Eto, Silas F.
Veras, Flávio P.
Fernandes, Bianca H. V.
Sanches, Paulo R. S. [UNESP]
Cilli, Eduardo M. [UNESP]
Malafaia, Guilherme
Nóbrega, Rafael H. [UNESP]
Garcez, Aguinaldo S.
Carrilho, Emanuel
Charlie-Silva, Ives [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
São Leopoldo Mandic College
Universidade de São Paulo (USP)
INCTBio
Oswaldo Cruz Foundation (Fiocruz)
Universidade de Passo Fundo
Federal University Minas Gerais
Nord University
Federal University of Jatai
Universidade Federal de Pernambuco (UFPE)
Federal University of Alfenas
FIOCRUZ Paraná
Laboratory of Development and Innovation Butantan Institute
Goiano Federal Institute
dc.contributor.author.fl_str_mv Rosa, Ivana F. [UNESP]
Peçanha, Ana P. B.
Carvalho, Tábata R. B.
Alexandre, Leonardo S.
Ferreira, Vinícius G.
Doretto, Lucas B. [UNESP]
Souza, Beatriz M. [UNESP]
Nakajima, Rafael T. [UNESP]
da Silva, Patrick
Barbosa, Ana P.
Gomes-de-Pontes, Leticia
Bomfim, Camila G.
Machado-Santelli, Glaucia M.
Condino-Neto, Antonio
Guzzo, Cristiane R.
Peron, Jean P. S.
Andrade-Silva, Magaiver
Câmara, Niels O. S.
Garnique, Anali M. B.
Medeiros, Renata J.
Ferraris, Fausto K.
Barcellos, Leonardo J. G.
Correia-Junior, Jose D.
Galindo-Villegas, Jorge
Machado, Mônica F. R.
Castoldi, Angela
Oliveira, Susana L. [UNESP]
Costa, Camila C. [UNESP]
Belo, Marco A. A. [UNESP]
Galdino, Giovane
Sgro, Germán G.
Bueno, Natalia F.
Eto, Silas F.
Veras, Flávio P.
Fernandes, Bianca H. V.
Sanches, Paulo R. S. [UNESP]
Cilli, Eduardo M. [UNESP]
Malafaia, Guilherme
Nóbrega, Rafael H. [UNESP]
Garcez, Aguinaldo S.
Carrilho, Emanuel
Charlie-Silva, Ives [UNESP]
dc.subject.por.fl_str_mv COVID-19
cytokine storms
oxidative stress
photobiomodulation
rSpike
zebrafish
topic COVID-19
cytokine storms
oxidative stress
photobiomodulation
rSpike
zebrafish
description Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:51:36Z
2023-07-29T13:51:36Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ijms24076104
International Journal of Molecular Sciences, v. 24, n. 7, 2023.
1422-0067
1661-6596
http://hdl.handle.net/11449/248713
10.3390/ijms24076104
2-s2.0-85152799400
url http://dx.doi.org/10.3390/ijms24076104
http://hdl.handle.net/11449/248713
identifier_str_mv International Journal of Molecular Sciences, v. 24, n. 7, 2023.
1422-0067
1661-6596
10.3390/ijms24076104
2-s2.0-85152799400
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Molecular Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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