Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-017-0729-0 http://hdl.handle.net/11449/175470 |
Resumo: | The present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities. The results revealed low transportability in vitro, a high percentage of monocytes and eosinophils, and increased frequency of leukocytes and nuclear abnormalities in fish from the polluted pond. However, the frequency of thrombocytes and erythroblasts and the percentage of lymphocytes and neutrophils were significantly lower. It is possible to conclude that changes in fish are due to poor water quality and that these non-destructive biomarkers can be used for the biomonitoring of aquatic environments vulnerable to contamination. |
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Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pondAquatic pollutionBiomarkersCytotoxicityHematologyMucusOreochromis niloticusThe present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities. The results revealed low transportability in vitro, a high percentage of monocytes and eosinophils, and increased frequency of leukocytes and nuclear abnormalities in fish from the polluted pond. However, the frequency of thrombocytes and erythroblasts and the percentage of lymphocytes and neutrophils were significantly lower. It is possible to conclude that changes in fish are due to poor water quality and that these non-destructive biomarkers can be used for the biomonitoring of aquatic environments vulnerable to contamination.Pós-Graduacão em Ecogestão Campus Paraíso Universidade Paulista—UNIPEscola Municipal de Ensino Fundamental Olavo Fontoura, Prefeitura do Município de São PauloCampus do Litoral Paulista—Núcleo de Estudos em Poluição e Ecotoxicologia Aquática Universidade Estadual Paulista—UNESPFaculdade das Américas—FAMDepartamento de Patologia Faculdade de Medicina Laboratório de Investigação Médica LIM05 Universidade de São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira CésarCampus do Litoral Paulista—Núcleo de Estudos em Poluição e Ecotoxicologia Aquática Universidade Estadual Paulista—UNESPUniversidade Paulista—UNIPEscola Municipal de Ensino Fundamental Olavo FontouraUniversidade Estadual Paulista (Unesp)Faculdade das Américas—FAMUniversidade de São Paulo (USP)da Silva, Edison Bezerrada Silva Corrêa, Sandra Aparecidade Souza Abessa, Denis Moledo [UNESP]da Silva, Bruno Ferreira XavierRivero, Dolores Helena Rodriguez FerreiraSeriani, Robson2018-12-11T17:15:57Z2018-12-11T17:15:57Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2683-2690application/pdfhttp://dx.doi.org/10.1007/s11356-017-0729-0Environmental Science and Pollution Research, v. 25, n. 3, p. 2683-2690, 2018.1614-74990944-1344http://hdl.handle.net/11449/17547010.1007/s11356-017-0729-02-s2.0-850335610082-s2.0-85033561008.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2023-10-04T06:01:12Zoai:repositorio.unesp.br:11449/175470Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:56:57.715998Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
title |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
spellingShingle |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond da Silva, Edison Bezerra Aquatic pollution Biomarkers Cytotoxicity Hematology Mucus Oreochromis niloticus |
title_short |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
title_full |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
title_fullStr |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
title_full_unstemmed |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
title_sort |
Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond |
author |
da Silva, Edison Bezerra |
author_facet |
da Silva, Edison Bezerra da Silva Corrêa, Sandra Aparecida de Souza Abessa, Denis Moledo [UNESP] da Silva, Bruno Ferreira Xavier Rivero, Dolores Helena Rodriguez Ferreira Seriani, Robson |
author_role |
author |
author2 |
da Silva Corrêa, Sandra Aparecida de Souza Abessa, Denis Moledo [UNESP] da Silva, Bruno Ferreira Xavier Rivero, Dolores Helena Rodriguez Ferreira Seriani, Robson |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Paulista—UNIP Escola Municipal de Ensino Fundamental Olavo Fontoura Universidade Estadual Paulista (Unesp) Faculdade das Américas—FAM Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
da Silva, Edison Bezerra da Silva Corrêa, Sandra Aparecida de Souza Abessa, Denis Moledo [UNESP] da Silva, Bruno Ferreira Xavier Rivero, Dolores Helena Rodriguez Ferreira Seriani, Robson |
dc.subject.por.fl_str_mv |
Aquatic pollution Biomarkers Cytotoxicity Hematology Mucus Oreochromis niloticus |
topic |
Aquatic pollution Biomarkers Cytotoxicity Hematology Mucus Oreochromis niloticus |
description |
The present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities. The results revealed low transportability in vitro, a high percentage of monocytes and eosinophils, and increased frequency of leukocytes and nuclear abnormalities in fish from the polluted pond. However, the frequency of thrombocytes and erythroblasts and the percentage of lymphocytes and neutrophils were significantly lower. It is possible to conclude that changes in fish are due to poor water quality and that these non-destructive biomarkers can be used for the biomonitoring of aquatic environments vulnerable to contamination. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:57Z 2018-12-11T17:15:57Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-017-0729-0 Environmental Science and Pollution Research, v. 25, n. 3, p. 2683-2690, 2018. 1614-7499 0944-1344 http://hdl.handle.net/11449/175470 10.1007/s11356-017-0729-0 2-s2.0-85033561008 2-s2.0-85033561008.pdf |
url |
http://dx.doi.org/10.1007/s11356-017-0729-0 http://hdl.handle.net/11449/175470 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 25, n. 3, p. 2683-2690, 2018. 1614-7499 0944-1344 10.1007/s11356-017-0729-0 2-s2.0-85033561008 2-s2.0-85033561008.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2683-2690 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128293584502784 |