Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs

Detalhes bibliográficos
Autor(a) principal: Felix de Lima, Valeria Marcal [UNESP]
Data de Publicação: 2012
Outros Autores: Fattori, Karina Reinaldo [UNESP], de Souza, Faust [UNESP], Eugênio, Flávia de Rezende [UNESP], Patto dos Santos, Paulo Sergio [UNESP], Rozza, Daniele Bernadete [UNESP], Machado, Gisele Fabrino [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2011.08.024
http://hdl.handle.net/11449/24742
Resumo: Dogs are the main domestic reservoirs of L. (L) chagasi. Once in the vertebrate host, the parasite may cause visceral leishmaniasis, which can also be transmitted to humans. Infected symptomatic dogs show disorganization in the white pulp in spleen tissue and a reduction in T lymphocytes in peripheral blood. To investigate whether apoptosis is involved in white pulp disorganization and diminished T cell counts in peripheral blood, apoptotic T cells from the spleen and peripheral blood of dogs naturally infected with L. (L) chagasi and presenting clinical manifestations were quantified and compared with healthy dogs. Thirteen symptomatic adult dogs infected by L. (L) chagasi and six healthy dogs from a nonendemic area (controls) were included in the study. Samples from spleen and peripheral blood were used to quantify apoptosis in CD3 lymphocytes by flow cytometry using Anexin V and Multicas-pase kits; the results were compared using the Mann Whitney test. The percentage of total T cells was lower in Leishmania infected dogs compared to healthy controls (P<0.05). Apoptosis levels in T cells from PBMC and spleen were higher in infected dogs than in controls (P < 0.05). The least squares method test was used to determine the effect between the degree of structural organization of spleen white pulp and the percentage of apoptosis in the spleen. A significant effect on the level of white pulp morphological disorganization and percentage of apoptosis in spleen T cells was observed (F = 20.45: P = 0.0014). These data suggest that apoptosis is an important for the immunopathogenesis of canine visceral leishmaniasis. (C) 2011 Elsevier B.V. All rights reserved.
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spelling Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogsLeishmaniasisApoptosisDogsSpleenPeripheral bloodDogs are the main domestic reservoirs of L. (L) chagasi. Once in the vertebrate host, the parasite may cause visceral leishmaniasis, which can also be transmitted to humans. Infected symptomatic dogs show disorganization in the white pulp in spleen tissue and a reduction in T lymphocytes in peripheral blood. To investigate whether apoptosis is involved in white pulp disorganization and diminished T cell counts in peripheral blood, apoptotic T cells from the spleen and peripheral blood of dogs naturally infected with L. (L) chagasi and presenting clinical manifestations were quantified and compared with healthy dogs. Thirteen symptomatic adult dogs infected by L. (L) chagasi and six healthy dogs from a nonendemic area (controls) were included in the study. Samples from spleen and peripheral blood were used to quantify apoptosis in CD3 lymphocytes by flow cytometry using Anexin V and Multicas-pase kits; the results were compared using the Mann Whitney test. The percentage of total T cells was lower in Leishmania infected dogs compared to healthy controls (P<0.05). Apoptosis levels in T cells from PBMC and spleen were higher in infected dogs than in controls (P < 0.05). The least squares method test was used to determine the effect between the degree of structural organization of spleen white pulp and the percentage of apoptosis in the spleen. A significant effect on the level of white pulp morphological disorganization and percentage of apoptosis in spleen T cells was observed (F = 20.45: P = 0.0014). These data suggest that apoptosis is an important for the immunopathogenesis of canine visceral leishmaniasis. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Univ Estadual Paulista, Inst Fis Teor, Dept Clin Cirurgia & Reprod Anim, Fac Vet Med, BR-16050400 São Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr Vet, Programa Posgrad Microbiol Agr, BR-14884900 São Paulo, BrazilUniv Estadual Paulista, Inst Fis Teor, Dept Clin Cirurgia & Reprod Anim, Fac Vet Med, BR-16050400 São Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr Vet, Programa Posgrad Microbiol Agr, BR-14884900 São Paulo, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Felix de Lima, Valeria Marcal [UNESP]Fattori, Karina Reinaldo [UNESP]de Souza, Faust [UNESP]Eugênio, Flávia de Rezende [UNESP]Patto dos Santos, Paulo Sergio [UNESP]Rozza, Daniele Bernadete [UNESP]Machado, Gisele Fabrino [UNESP]2013-09-30T19:05:11Z2014-05-20T14:15:04Z2013-09-30T19:05:11Z2014-05-20T14:15:04Z2012-03-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-153application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2011.08.024Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 184, n. 2-4, p. 147-153, 2012.0304-4017http://hdl.handle.net/11449/2474210.1016/j.vetpar.2011.08.024WOS:000300331600008WOS000300331600008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2023-10-17T06:04:03Zoai:repositorio.unesp.br:11449/24742Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:16.487099Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
title Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
spellingShingle Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
Felix de Lima, Valeria Marcal [UNESP]
Leishmaniasis
Apoptosis
Dogs
Spleen
Peripheral blood
title_short Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
title_full Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
title_fullStr Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
title_full_unstemmed Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
title_sort Apoptosis in T lymphocytes from spleen tissue and peripheral blood of L. (L.) chagasi naturally infected dogs
author Felix de Lima, Valeria Marcal [UNESP]
author_facet Felix de Lima, Valeria Marcal [UNESP]
Fattori, Karina Reinaldo [UNESP]
de Souza, Faust [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto dos Santos, Paulo Sergio [UNESP]
Rozza, Daniele Bernadete [UNESP]
Machado, Gisele Fabrino [UNESP]
author_role author
author2 Fattori, Karina Reinaldo [UNESP]
de Souza, Faust [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto dos Santos, Paulo Sergio [UNESP]
Rozza, Daniele Bernadete [UNESP]
Machado, Gisele Fabrino [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Felix de Lima, Valeria Marcal [UNESP]
Fattori, Karina Reinaldo [UNESP]
de Souza, Faust [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto dos Santos, Paulo Sergio [UNESP]
Rozza, Daniele Bernadete [UNESP]
Machado, Gisele Fabrino [UNESP]
dc.subject.por.fl_str_mv Leishmaniasis
Apoptosis
Dogs
Spleen
Peripheral blood
topic Leishmaniasis
Apoptosis
Dogs
Spleen
Peripheral blood
description Dogs are the main domestic reservoirs of L. (L) chagasi. Once in the vertebrate host, the parasite may cause visceral leishmaniasis, which can also be transmitted to humans. Infected symptomatic dogs show disorganization in the white pulp in spleen tissue and a reduction in T lymphocytes in peripheral blood. To investigate whether apoptosis is involved in white pulp disorganization and diminished T cell counts in peripheral blood, apoptotic T cells from the spleen and peripheral blood of dogs naturally infected with L. (L) chagasi and presenting clinical manifestations were quantified and compared with healthy dogs. Thirteen symptomatic adult dogs infected by L. (L) chagasi and six healthy dogs from a nonendemic area (controls) were included in the study. Samples from spleen and peripheral blood were used to quantify apoptosis in CD3 lymphocytes by flow cytometry using Anexin V and Multicas-pase kits; the results were compared using the Mann Whitney test. The percentage of total T cells was lower in Leishmania infected dogs compared to healthy controls (P<0.05). Apoptosis levels in T cells from PBMC and spleen were higher in infected dogs than in controls (P < 0.05). The least squares method test was used to determine the effect between the degree of structural organization of spleen white pulp and the percentage of apoptosis in the spleen. A significant effect on the level of white pulp morphological disorganization and percentage of apoptosis in spleen T cells was observed (F = 20.45: P = 0.0014). These data suggest that apoptosis is an important for the immunopathogenesis of canine visceral leishmaniasis. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-23
2013-09-30T19:05:11Z
2013-09-30T19:05:11Z
2014-05-20T14:15:04Z
2014-05-20T14:15:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2011.08.024
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 184, n. 2-4, p. 147-153, 2012.
0304-4017
http://hdl.handle.net/11449/24742
10.1016/j.vetpar.2011.08.024
WOS:000300331600008
WOS000300331600008.pdf
url http://dx.doi.org/10.1016/j.vetpar.2011.08.024
http://hdl.handle.net/11449/24742
identifier_str_mv Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 184, n. 2-4, p. 147-153, 2012.
0304-4017
10.1016/j.vetpar.2011.08.024
WOS:000300331600008
WOS000300331600008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
2.422
1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-153
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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