Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Bruno V.M.
Data de Publicação: 2016
Outros Autores: Silva, Aline S., Melo, Gabriela F.S. [UNESP], Vasconscellos, Luana M.R. [UNESP], Marciano, Fernanda R., Lobo, Anderson O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.msec.2015.10.075
http://hdl.handle.net/11449/172174
Resumo: The use of poly (butylene adipate-co-terephthalate) (PBAT) in tissue engineering, more specifically in bone regeneration, has been underexplored to date due to its poor mechanical resistance. In order to overcome this drawback, this investigation presents an approach into the preparation of electrospun nanocomposite fibers from PBAT and low contents of superhydrophilic multi-walled carbon nanotubes (sMWCNT) (0.1-0.5 wt.%) as reinforcing agent. We employed a wide range of characterization techniques to evaluate the properties of the resulting electrospun nanocomposites, including Field Emission Scanning Electronic Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM), tensile tests, contact angle measurements (CA) and biological assays. FE-SEM micrographs showed that while the addition of sMWCNT increased the presence of beads on the electrospun fibers' surfaces, the increase of the neat charge density due to their presence reduced the fibers' average diameter. The tensile test results pointed that sMWCNT acted as reinforcement in the PBAT electrospun matrix, enhancing its tensile strength (from 1.3 to 3.6 MPa with addition of 0.5 wt.% of sMWCNT) and leading to stiffer materials (lower elongation at break). An evaluation using MG63 cells revealed cell attachment into the biomaterials and that all samples were viable for biomedical applications, once no cytotoxic effect was observed. MG-63 cells osteogenic differentiation, measured by ALP activity, showed that mineralized nodules formation was increased in PBAT/0.5%CNTs when compared to control group (cells). This investigation demonstrated a feasible novel approach for producing electrospun nanocomposites from PBAT and sMWCNT with enhanced mechanical properties and adequate cell viability levels, which allows for a wide range of biomedical applications for these materials.
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spelling Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibersElectrospinningMechanical properties, osteogenesisNanocompositesPBATSuperhydrophilic MWCNTThe use of poly (butylene adipate-co-terephthalate) (PBAT) in tissue engineering, more specifically in bone regeneration, has been underexplored to date due to its poor mechanical resistance. In order to overcome this drawback, this investigation presents an approach into the preparation of electrospun nanocomposite fibers from PBAT and low contents of superhydrophilic multi-walled carbon nanotubes (sMWCNT) (0.1-0.5 wt.%) as reinforcing agent. We employed a wide range of characterization techniques to evaluate the properties of the resulting electrospun nanocomposites, including Field Emission Scanning Electronic Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM), tensile tests, contact angle measurements (CA) and biological assays. FE-SEM micrographs showed that while the addition of sMWCNT increased the presence of beads on the electrospun fibers' surfaces, the increase of the neat charge density due to their presence reduced the fibers' average diameter. The tensile test results pointed that sMWCNT acted as reinforcement in the PBAT electrospun matrix, enhancing its tensile strength (from 1.3 to 3.6 MPa with addition of 0.5 wt.% of sMWCNT) and leading to stiffer materials (lower elongation at break). An evaluation using MG63 cells revealed cell attachment into the biomaterials and that all samples were viable for biomedical applications, once no cytotoxic effect was observed. MG-63 cells osteogenic differentiation, measured by ALP activity, showed that mineralized nodules formation was increased in PBAT/0.5%CNTs when compared to control group (cells). This investigation demonstrated a feasible novel approach for producing electrospun nanocomposites from PBAT and sMWCNT with enhanced mechanical properties and adequate cell viability levels, which allows for a wide range of biomedical applications for these materials.Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IPandD), University of Vale Do Paraiba (UNIVAP), Sao Jose Dos Campos, Av. Shishima Hifumi 2911Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University (UNESP), Sao Jose Dos Campos, Av. Engenheiro Francisco Jose Longo 777Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University (UNESP), Sao Jose Dos Campos, Av. Engenheiro Francisco Jose Longo 777Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IPandD), University of Vale Do Paraiba (UNIVAP), Sao Jose Dos CamposUniversidade Estadual Paulista (Unesp)Rodrigues, Bruno V.M.Silva, Aline S.Melo, Gabriela F.S. [UNESP]Vasconscellos, Luana M.R. [UNESP]Marciano, Fernanda R.Lobo, Anderson O.2018-12-11T16:59:02Z2018-12-11T16:59:02Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article782-791application/pdfhttp://dx.doi.org/10.1016/j.msec.2015.10.075Materials Science and Engineering C, v. 59, p. 782-791.0928-4931http://hdl.handle.net/11449/17217410.1016/j.msec.2015.10.0752-s2.0-849461033872-s2.0-84946103387.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering C1,110info:eu-repo/semantics/openAccess2023-11-16T06:07:31Zoai:repositorio.unesp.br:11449/172174Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:47:46.419771Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
title Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
spellingShingle Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
Rodrigues, Bruno V.M.
Electrospinning
Mechanical properties, osteogenesis
Nanocomposites
PBAT
Superhydrophilic MWCNT
title_short Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
title_full Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
title_fullStr Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
title_full_unstemmed Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
title_sort Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers
author Rodrigues, Bruno V.M.
author_facet Rodrigues, Bruno V.M.
Silva, Aline S.
Melo, Gabriela F.S. [UNESP]
Vasconscellos, Luana M.R. [UNESP]
Marciano, Fernanda R.
Lobo, Anderson O.
author_role author
author2 Silva, Aline S.
Melo, Gabriela F.S. [UNESP]
Vasconscellos, Luana M.R. [UNESP]
Marciano, Fernanda R.
Lobo, Anderson O.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IPandD), University of Vale Do Paraiba (UNIVAP), Sao Jose Dos Campos
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rodrigues, Bruno V.M.
Silva, Aline S.
Melo, Gabriela F.S. [UNESP]
Vasconscellos, Luana M.R. [UNESP]
Marciano, Fernanda R.
Lobo, Anderson O.
dc.subject.por.fl_str_mv Electrospinning
Mechanical properties, osteogenesis
Nanocomposites
PBAT
Superhydrophilic MWCNT
topic Electrospinning
Mechanical properties, osteogenesis
Nanocomposites
PBAT
Superhydrophilic MWCNT
description The use of poly (butylene adipate-co-terephthalate) (PBAT) in tissue engineering, more specifically in bone regeneration, has been underexplored to date due to its poor mechanical resistance. In order to overcome this drawback, this investigation presents an approach into the preparation of electrospun nanocomposite fibers from PBAT and low contents of superhydrophilic multi-walled carbon nanotubes (sMWCNT) (0.1-0.5 wt.%) as reinforcing agent. We employed a wide range of characterization techniques to evaluate the properties of the resulting electrospun nanocomposites, including Field Emission Scanning Electronic Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM), tensile tests, contact angle measurements (CA) and biological assays. FE-SEM micrographs showed that while the addition of sMWCNT increased the presence of beads on the electrospun fibers' surfaces, the increase of the neat charge density due to their presence reduced the fibers' average diameter. The tensile test results pointed that sMWCNT acted as reinforcement in the PBAT electrospun matrix, enhancing its tensile strength (from 1.3 to 3.6 MPa with addition of 0.5 wt.% of sMWCNT) and leading to stiffer materials (lower elongation at break). An evaluation using MG63 cells revealed cell attachment into the biomaterials and that all samples were viable for biomedical applications, once no cytotoxic effect was observed. MG-63 cells osteogenic differentiation, measured by ALP activity, showed that mineralized nodules formation was increased in PBAT/0.5%CNTs when compared to control group (cells). This investigation demonstrated a feasible novel approach for producing electrospun nanocomposites from PBAT and sMWCNT with enhanced mechanical properties and adequate cell viability levels, which allows for a wide range of biomedical applications for these materials.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
2018-12-11T16:59:02Z
2018-12-11T16:59:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.msec.2015.10.075
Materials Science and Engineering C, v. 59, p. 782-791.
0928-4931
http://hdl.handle.net/11449/172174
10.1016/j.msec.2015.10.075
2-s2.0-84946103387
2-s2.0-84946103387.pdf
url http://dx.doi.org/10.1016/j.msec.2015.10.075
http://hdl.handle.net/11449/172174
identifier_str_mv Materials Science and Engineering C, v. 59, p. 782-791.
0928-4931
10.1016/j.msec.2015.10.075
2-s2.0-84946103387
2-s2.0-84946103387.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Science and Engineering C
1,110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 782-791
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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