Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000600006&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/127397 |
Resumo: | This study analysed the effect of pastes formulated with calcium hydroxide P.A. and different vehicles (saline solution - paste A and Copaifera langsdorffii Desfon oil - paste B) on oral microorganisms and dentin bridge formation in dogs. The antimicrobial action of the pastes and their components was analysed by the minimum inhibitory concentration in agar gel technique. The components were diluted and tested on fifteen standard strains of microorganisms associated with endodontic diseases. The microorganisms were cultivated and after incubation data was analysed using One-Way ANOVA and Turkey's test (P≤0.05). Four superior incisors of ten animals were used to evaluate dentin bridge formation. Two incisors were capped with paste A (GA) and two with paste B (GB). After 90 days, the teeth were extracted for histological analysis and the degree of dentin bridge formation evaluated. Data was analysed by the Kruskal-Wallis test (P<0.05). The pastes and their components were classified in the following decreasing order of antimicrobial action: calcium hydroxide P.A., paste A, paste B and Copaifera langsdorffii Desfon oil. Calcium hydroxide P.A. showed significantly higher antimicrobial action than the pastes or their vehicles. No significant difference was observed between the two pastes in dentin bridge formation. Based on the microorganisms studied, it can be concluded that the pastes analysed showed similar antimicrobial potential but differed significantly from their individual components. No significant difference was observed in dentin bridge formation between the different pastes tested. |
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Repositório Institucional da UNESP |
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Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogsPastas endodônticas formuladas com óleo de copaíba: ação na microbiota bucal e formação de ponte dentinária em cãesMicrobiologyDental pulp cappingMedicinal plantsMicrobiologiaCapeamento da polpa dentáriaPlantas medicinaisThis study analysed the effect of pastes formulated with calcium hydroxide P.A. and different vehicles (saline solution - paste A and Copaifera langsdorffii Desfon oil - paste B) on oral microorganisms and dentin bridge formation in dogs. The antimicrobial action of the pastes and their components was analysed by the minimum inhibitory concentration in agar gel technique. The components were diluted and tested on fifteen standard strains of microorganisms associated with endodontic diseases. The microorganisms were cultivated and after incubation data was analysed using One-Way ANOVA and Turkey's test (P≤0.05). Four superior incisors of ten animals were used to evaluate dentin bridge formation. Two incisors were capped with paste A (GA) and two with paste B (GB). After 90 days, the teeth were extracted for histological analysis and the degree of dentin bridge formation evaluated. Data was analysed by the Kruskal-Wallis test (P<0.05). The pastes and their components were classified in the following decreasing order of antimicrobial action: calcium hydroxide P.A., paste A, paste B and Copaifera langsdorffii Desfon oil. Calcium hydroxide P.A. showed significantly higher antimicrobial action than the pastes or their vehicles. No significant difference was observed between the two pastes in dentin bridge formation. Based on the microorganisms studied, it can be concluded that the pastes analysed showed similar antimicrobial potential but differed significantly from their individual components. No significant difference was observed in dentin bridge formation between the different pastes tested.Foi avaliada a ação de pastas formuladas com hidróxido de cálcio P.A. e diferentes veículos (solução fisiológica - pasta A e óleo de Copaifera langsdorffii Desfon - pasta B) sobre microrganismos bucais e formação de ponte dentinária em cães. A ação antimicrobiana das pastas e de seus componentes individuais foi avaliada pela técnica da concentração inibitória mínima pela diluição em ágar. Os materiais foram diluídos e avaliados contra quinze cepas padrão de microrganismos relacionados a doenças endodônticas. Os microrganismos foram cultivados e, após a incubação, os dados foram analisados (Anova One-Way, Tukey, P≤0,05). Para a avaliação da formação de ponte dentinária, quatro incisivos superiores de dez animais foram tratados, sendo dois capeados com pasta A (GA) e dois com a pasta B (GB). Após 90 dias, os dentes foram extraídos para obtenção de cortes histológicos, com o objetivo de se avaliar o grau de formação de ponte dentinária. Os dados obtidos foram submetidos à análise estatística (Kruskal-Wallis, P<0,05). Segundo a ação antimicrobiana, os materiais foram classificados em ordem decrescente: hidróxido de cálcio P.A., pasta A, pasta B e óleo de Copaifera langsdorffii Desfon, com diferenças estatisticamente significantes entre o hidróxido de cálcio P.A., as pastas e os veículos. Ao final do estudo, observou-se que as duas pastas avaliadas foram semelhantes quanto à formação de ponte dentinária. Considerando-se os microrganismos selecionados, conclui-se que as pastas avaliadas foram semelhantes entre si em termos de potencial antimicrobiano, diferindo dos componentes individuais. Em relação à formação de ponte dentinária, não foram observadas diferenças significantes entre os dentes tratados com as diferentes pastas.Universidade de Franca (UNIFRAN) Programa de Pós-graduação em CiênciasUNIFRAN Curso de OdontologiaUniversidade Estadual Paulista (UNESP) Departamento de Clínica e Cirurgia VeterináriaUNIFRAN Programa de Pós-graduação em Ciência AnimalUNESP Departamento de Reprodução AnimalUniversidade Estadual Paulista (UNESP) Departamento de Clínica e Cirurgia VeterináriaUNESP Departamento de Reprodução AnimalUniversidade Federal de Santa MariaUniversidade de Franca (UNIFRAN)Universidade Estadual Paulista (Unesp)Dias, Fernanda Gosuen GonçalvesCasemiro, Luciana AssiratiMartins, Carlos Henrique GomesDias, Luis Gustavo Gosuen Gonçalves [UNESP]Pereira, Lucas De FreitasNishimura, Lilian ToshikoSouza, Fabiana Ferreira De [UNESP]Honsho, Cristiane Dos Santos2015-08-26T19:21:43Z2015-08-26T19:21:43Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1073-1078application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000600006&lng=en&nrm=iso&tlng=enCiência Rural, v. 45, n. 6, p. 1073-1078, 2015.0103-8478http://hdl.handle.net/11449/12739710.1590/0103-8478cr20141284S0103-84782015000601073S0103-84782015000601073.pdf627611148293541472945822894030490000-0003-3993-16490000-0003-4721-1801SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiência Rural0.5250,337info:eu-repo/semantics/openAccess2023-12-05T06:18:41Zoai:repositorio.unesp.br:11449/127397Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-05T06:18:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs Pastas endodônticas formuladas com óleo de copaíba: ação na microbiota bucal e formação de ponte dentinária em cães |
title |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
spellingShingle |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs Dias, Fernanda Gosuen Gonçalves Microbiology Dental pulp capping Medicinal plants Microbiologia Capeamento da polpa dentária Plantas medicinais |
title_short |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
title_full |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
title_fullStr |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
title_full_unstemmed |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
title_sort |
Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs |
author |
Dias, Fernanda Gosuen Gonçalves |
author_facet |
Dias, Fernanda Gosuen Gonçalves Casemiro, Luciana Assirati Martins, Carlos Henrique Gomes Dias, Luis Gustavo Gosuen Gonçalves [UNESP] Pereira, Lucas De Freitas Nishimura, Lilian Toshiko Souza, Fabiana Ferreira De [UNESP] Honsho, Cristiane Dos Santos |
author_role |
author |
author2 |
Casemiro, Luciana Assirati Martins, Carlos Henrique Gomes Dias, Luis Gustavo Gosuen Gonçalves [UNESP] Pereira, Lucas De Freitas Nishimura, Lilian Toshiko Souza, Fabiana Ferreira De [UNESP] Honsho, Cristiane Dos Santos |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Franca (UNIFRAN) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dias, Fernanda Gosuen Gonçalves Casemiro, Luciana Assirati Martins, Carlos Henrique Gomes Dias, Luis Gustavo Gosuen Gonçalves [UNESP] Pereira, Lucas De Freitas Nishimura, Lilian Toshiko Souza, Fabiana Ferreira De [UNESP] Honsho, Cristiane Dos Santos |
dc.subject.por.fl_str_mv |
Microbiology Dental pulp capping Medicinal plants Microbiologia Capeamento da polpa dentária Plantas medicinais |
topic |
Microbiology Dental pulp capping Medicinal plants Microbiologia Capeamento da polpa dentária Plantas medicinais |
description |
This study analysed the effect of pastes formulated with calcium hydroxide P.A. and different vehicles (saline solution - paste A and Copaifera langsdorffii Desfon oil - paste B) on oral microorganisms and dentin bridge formation in dogs. The antimicrobial action of the pastes and their components was analysed by the minimum inhibitory concentration in agar gel technique. The components were diluted and tested on fifteen standard strains of microorganisms associated with endodontic diseases. The microorganisms were cultivated and after incubation data was analysed using One-Way ANOVA and Turkey's test (P≤0.05). Four superior incisors of ten animals were used to evaluate dentin bridge formation. Two incisors were capped with paste A (GA) and two with paste B (GB). After 90 days, the teeth were extracted for histological analysis and the degree of dentin bridge formation evaluated. Data was analysed by the Kruskal-Wallis test (P<0.05). The pastes and their components were classified in the following decreasing order of antimicrobial action: calcium hydroxide P.A., paste A, paste B and Copaifera langsdorffii Desfon oil. Calcium hydroxide P.A. showed significantly higher antimicrobial action than the pastes or their vehicles. No significant difference was observed between the two pastes in dentin bridge formation. Based on the microorganisms studied, it can be concluded that the pastes analysed showed similar antimicrobial potential but differed significantly from their individual components. No significant difference was observed in dentin bridge formation between the different pastes tested. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-26T19:21:43Z 2015-08-26T19:21:43Z 2015-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000600006&lng=en&nrm=iso&tlng=en Ciência Rural, v. 45, n. 6, p. 1073-1078, 2015. 0103-8478 http://hdl.handle.net/11449/127397 10.1590/0103-8478cr20141284 S0103-84782015000601073 S0103-84782015000601073.pdf 6276111482935414 7294582289403049 0000-0003-3993-1649 0000-0003-4721-1801 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000600006&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/127397 |
identifier_str_mv |
Ciência Rural, v. 45, n. 6, p. 1073-1078, 2015. 0103-8478 10.1590/0103-8478cr20141284 S0103-84782015000601073 S0103-84782015000601073.pdf 6276111482935414 7294582289403049 0000-0003-3993-1649 0000-0003-4721-1801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciência Rural 0.525 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1073-1078 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046809001525248 |