Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10787-018-0443-7 http://hdl.handle.net/11449/170674 |
Resumo: | The study aimed to evaluate the metabolic and inflammatory effects of short-term treatments (10 days) with metformin (MET) on the NAFLD caused by a high-fat diet (HFD) in C57BL/6 mice. After the treatment, histological liver slices were obtained, hepatocytes and macrophages were extracted and cultured with phosphate buffered saline, LPS (2.5 µg/mL) and MET (1 µM) for 24 h. Cytokine levels were determined by ELISA. NAFLD caused by the HFD was partially reduced by MET. The lipid accumulation induced by the HFD was not associated with liver inflammation; however, MET seemed to promote pro-inflammatory effects in liver, since it increased hepatic concentration of IL-1β, TNF-α, IL-6, MCP-1 and IFN-γ. Similarly, MET increased the concentration of IL-1β, IL-6 in hepatocyte cultures. However, in macrophages culture, MET lowered levels of IL-1β, IL-6 and TNF-α stimulated by LPS. Overall, MET reduced liver NAFLD but promoted hepatocyte increase in pro-inflammatory cytokines, thus, leading to liver inflammation. |
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Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese miceInflammationLiverMetforminObesityThe study aimed to evaluate the metabolic and inflammatory effects of short-term treatments (10 days) with metformin (MET) on the NAFLD caused by a high-fat diet (HFD) in C57BL/6 mice. After the treatment, histological liver slices were obtained, hepatocytes and macrophages were extracted and cultured with phosphate buffered saline, LPS (2.5 µg/mL) and MET (1 µM) for 24 h. Cytokine levels were determined by ELISA. NAFLD caused by the HFD was partially reduced by MET. The lipid accumulation induced by the HFD was not associated with liver inflammation; however, MET seemed to promote pro-inflammatory effects in liver, since it increased hepatic concentration of IL-1β, TNF-α, IL-6, MCP-1 and IFN-γ. Similarly, MET increased the concentration of IL-1β, IL-6 in hepatocyte cultures. However, in macrophages culture, MET lowered levels of IL-1β, IL-6 and TNF-α stimulated by LPS. Overall, MET reduced liver NAFLD but promoted hepatocyte increase in pro-inflammatory cytokines, thus, leading to liver inflammation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Immunometabolism Research Group Department of Cell Biology and Development Institute of Biomedical Sciences University of São PauloExercise and Immunometabolism Research Group Department of Physical Education UNESPDepartment of Physiology and Biophysics Institute of Biomedical Sciences University of São PauloCruzeiro do Sul UniversityExercise and Immunometabolism Research Group Department of Physical Education UNESPFAPESP: 2015/16777-0FAPESP: 2016/01409-8Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Cruzeiro do Sul Universityde Souza Teixeira, Alexandre AbilioSouza, Camila O.Biondo, Luana A.Sanches Silveira, Loreana [UNESP]Lima, Edson A.Batatinha, Helena A.Araujo, Adriane PereiraAlves, Michele JoanaHirabara, Sandro MassaoCuri, RuiNeto, José Cesar Rosa2018-12-11T16:51:56Z2018-12-11T16:51:56Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1103-1115application/pdfhttp://dx.doi.org/10.1007/s10787-018-0443-7Inflammopharmacology, v. 26, n. 4, p. 1103-1115, 2018.1568-56080925-4692http://hdl.handle.net/11449/17067410.1007/s10787-018-0443-72-s2.0-850421368212-s2.0-85042136821.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammopharmacology0,9250,925info:eu-repo/semantics/openAccess2023-11-24T06:13:42Zoai:repositorio.unesp.br:11449/170674Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:35:11.655956Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
title |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
spellingShingle |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice de Souza Teixeira, Alexandre Abilio Inflammation Liver Metformin Obesity |
title_short |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
title_full |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
title_fullStr |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
title_full_unstemmed |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
title_sort |
Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice |
author |
de Souza Teixeira, Alexandre Abilio |
author_facet |
de Souza Teixeira, Alexandre Abilio Souza, Camila O. Biondo, Luana A. Sanches Silveira, Loreana [UNESP] Lima, Edson A. Batatinha, Helena A. Araujo, Adriane Pereira Alves, Michele Joana Hirabara, Sandro Massao Curi, Rui Neto, José Cesar Rosa |
author_role |
author |
author2 |
Souza, Camila O. Biondo, Luana A. Sanches Silveira, Loreana [UNESP] Lima, Edson A. Batatinha, Helena A. Araujo, Adriane Pereira Alves, Michele Joana Hirabara, Sandro Massao Curi, Rui Neto, José Cesar Rosa |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Cruzeiro do Sul University |
dc.contributor.author.fl_str_mv |
de Souza Teixeira, Alexandre Abilio Souza, Camila O. Biondo, Luana A. Sanches Silveira, Loreana [UNESP] Lima, Edson A. Batatinha, Helena A. Araujo, Adriane Pereira Alves, Michele Joana Hirabara, Sandro Massao Curi, Rui Neto, José Cesar Rosa |
dc.subject.por.fl_str_mv |
Inflammation Liver Metformin Obesity |
topic |
Inflammation Liver Metformin Obesity |
description |
The study aimed to evaluate the metabolic and inflammatory effects of short-term treatments (10 days) with metformin (MET) on the NAFLD caused by a high-fat diet (HFD) in C57BL/6 mice. After the treatment, histological liver slices were obtained, hepatocytes and macrophages were extracted and cultured with phosphate buffered saline, LPS (2.5 µg/mL) and MET (1 µM) for 24 h. Cytokine levels were determined by ELISA. NAFLD caused by the HFD was partially reduced by MET. The lipid accumulation induced by the HFD was not associated with liver inflammation; however, MET seemed to promote pro-inflammatory effects in liver, since it increased hepatic concentration of IL-1β, TNF-α, IL-6, MCP-1 and IFN-γ. Similarly, MET increased the concentration of IL-1β, IL-6 in hepatocyte cultures. However, in macrophages culture, MET lowered levels of IL-1β, IL-6 and TNF-α stimulated by LPS. Overall, MET reduced liver NAFLD but promoted hepatocyte increase in pro-inflammatory cytokines, thus, leading to liver inflammation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:51:56Z 2018-12-11T16:51:56Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10787-018-0443-7 Inflammopharmacology, v. 26, n. 4, p. 1103-1115, 2018. 1568-5608 0925-4692 http://hdl.handle.net/11449/170674 10.1007/s10787-018-0443-7 2-s2.0-85042136821 2-s2.0-85042136821.pdf |
url |
http://dx.doi.org/10.1007/s10787-018-0443-7 http://hdl.handle.net/11449/170674 |
identifier_str_mv |
Inflammopharmacology, v. 26, n. 4, p. 1103-1115, 2018. 1568-5608 0925-4692 10.1007/s10787-018-0443-7 2-s2.0-85042136821 2-s2.0-85042136821.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Inflammopharmacology 0,925 0,925 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1103-1115 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128952313577472 |