Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3168/jds.2020-19979 http://hdl.handle.net/11449/206257 |
Resumo: | Sheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis. |
id |
UNSP_3159416707466659bee8201805510e97 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/206257 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesisazoxymethanecolon carcinogenesissheep dairy foodsynbioticSheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis.Department of Food Science and Nutrition Faculty of Food Engineering University of CampinasDepartment of Veterinary Hygiene and Technological Processing of Animal Products Veterinary School Federal Fluminense University (UFF)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Food Federal Institute of Rio de Janeiro (IFRJ)Department of Food Science Federal Institute of Paraná (IFPR), ParanavaíDepartment of Food Technology Rural Federal University of Rio de Janeiro (UFRRJ)Department of Agriculture Food Natural Resources and Engineering (DAFNE) University of Foggia (UNIFG)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)Federal Fluminense University (UFF)Universidade Estadual Paulista (Unesp)Federal Institute of Rio de Janeiro (IFRJ)Federal Institute of Paraná (IFPR)Rural Federal University of Rio de Janeiro (UFRRJ)University of Foggia (UNIFG)Balthazar, Celso F.de Moura, Nelci A. [UNESP]Romualdo, Guilherme R. [UNESP]Rocha, Ramon S.Pimentel, Tatiana C.Esmerino, Erick A.Freitas, Mônica Q.Santillo, AntonellaSilva, Marcia CristinaBarbisan, Luis F.Cruz, Adriano G.Albenzio, Marzia2021-06-25T10:29:10Z2021-06-25T10:29:10Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3168/jds.2020-19979Journal of Dairy Science.1525-31980022-0302http://hdl.handle.net/11449/20625710.3168/jds.2020-199792-s2.0-85104947682Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Scienceinfo:eu-repo/semantics/openAccess2021-10-23T02:05:30Zoai:repositorio.unesp.br:11449/206257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:54.027691Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
title |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
spellingShingle |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis Balthazar, Celso F. azoxymethane colon carcinogenesis sheep dairy food synbiotic |
title_short |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
title_full |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
title_fullStr |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
title_full_unstemmed |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
title_sort |
Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis |
author |
Balthazar, Celso F. |
author_facet |
Balthazar, Celso F. de Moura, Nelci A. [UNESP] Romualdo, Guilherme R. [UNESP] Rocha, Ramon S. Pimentel, Tatiana C. Esmerino, Erick A. Freitas, Mônica Q. Santillo, Antonella Silva, Marcia Cristina Barbisan, Luis F. Cruz, Adriano G. Albenzio, Marzia |
author_role |
author |
author2 |
de Moura, Nelci A. [UNESP] Romualdo, Guilherme R. [UNESP] Rocha, Ramon S. Pimentel, Tatiana C. Esmerino, Erick A. Freitas, Mônica Q. Santillo, Antonella Silva, Marcia Cristina Barbisan, Luis F. Cruz, Adriano G. Albenzio, Marzia |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Federal Fluminense University (UFF) Universidade Estadual Paulista (Unesp) Federal Institute of Rio de Janeiro (IFRJ) Federal Institute of Paraná (IFPR) Rural Federal University of Rio de Janeiro (UFRRJ) University of Foggia (UNIFG) |
dc.contributor.author.fl_str_mv |
Balthazar, Celso F. de Moura, Nelci A. [UNESP] Romualdo, Guilherme R. [UNESP] Rocha, Ramon S. Pimentel, Tatiana C. Esmerino, Erick A. Freitas, Mônica Q. Santillo, Antonella Silva, Marcia Cristina Barbisan, Luis F. Cruz, Adriano G. Albenzio, Marzia |
dc.subject.por.fl_str_mv |
azoxymethane colon carcinogenesis sheep dairy food synbiotic |
topic |
azoxymethane colon carcinogenesis sheep dairy food synbiotic |
description |
Sheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:29:10Z 2021-06-25T10:29:10Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3168/jds.2020-19979 Journal of Dairy Science. 1525-3198 0022-0302 http://hdl.handle.net/11449/206257 10.3168/jds.2020-19979 2-s2.0-85104947682 |
url |
http://dx.doi.org/10.3168/jds.2020-19979 http://hdl.handle.net/11449/206257 |
identifier_str_mv |
Journal of Dairy Science. 1525-3198 0022-0302 10.3168/jds.2020-19979 2-s2.0-85104947682 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dairy Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129516639354880 |