Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis

Detalhes bibliográficos
Autor(a) principal: Balthazar, Celso F.
Data de Publicação: 2021
Outros Autores: de Moura, Nelci A. [UNESP], Romualdo, Guilherme R. [UNESP], Rocha, Ramon S., Pimentel, Tatiana C., Esmerino, Erick A., Freitas, Mônica Q., Santillo, Antonella, Silva, Marcia Cristina, Barbisan, Luis F., Cruz, Adriano G., Albenzio, Marzia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3168/jds.2020-19979
http://hdl.handle.net/11449/206257
Resumo: Sheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis.
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spelling Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesisazoxymethanecolon carcinogenesissheep dairy foodsynbioticSheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis.Department of Food Science and Nutrition Faculty of Food Engineering University of CampinasDepartment of Veterinary Hygiene and Technological Processing of Animal Products Veterinary School Federal Fluminense University (UFF)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Food Federal Institute of Rio de Janeiro (IFRJ)Department of Food Science Federal Institute of Paraná (IFPR), ParanavaíDepartment of Food Technology Rural Federal University of Rio de Janeiro (UFRRJ)Department of Agriculture Food Natural Resources and Engineering (DAFNE) University of Foggia (UNIFG)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)Federal Fluminense University (UFF)Universidade Estadual Paulista (Unesp)Federal Institute of Rio de Janeiro (IFRJ)Federal Institute of Paraná (IFPR)Rural Federal University of Rio de Janeiro (UFRRJ)University of Foggia (UNIFG)Balthazar, Celso F.de Moura, Nelci A. [UNESP]Romualdo, Guilherme R. [UNESP]Rocha, Ramon S.Pimentel, Tatiana C.Esmerino, Erick A.Freitas, Mônica Q.Santillo, AntonellaSilva, Marcia CristinaBarbisan, Luis F.Cruz, Adriano G.Albenzio, Marzia2021-06-25T10:29:10Z2021-06-25T10:29:10Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3168/jds.2020-19979Journal of Dairy Science.1525-31980022-0302http://hdl.handle.net/11449/20625710.3168/jds.2020-199792-s2.0-85104947682Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Scienceinfo:eu-repo/semantics/openAccess2021-10-23T02:05:30Zoai:repositorio.unesp.br:11449/206257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:54.027691Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
title Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
spellingShingle Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
Balthazar, Celso F.
azoxymethane
colon carcinogenesis
sheep dairy food
synbiotic
title_short Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
title_full Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
title_fullStr Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
title_full_unstemmed Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
title_sort Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis
author Balthazar, Celso F.
author_facet Balthazar, Celso F.
de Moura, Nelci A. [UNESP]
Romualdo, Guilherme R. [UNESP]
Rocha, Ramon S.
Pimentel, Tatiana C.
Esmerino, Erick A.
Freitas, Mônica Q.
Santillo, Antonella
Silva, Marcia Cristina
Barbisan, Luis F.
Cruz, Adriano G.
Albenzio, Marzia
author_role author
author2 de Moura, Nelci A. [UNESP]
Romualdo, Guilherme R. [UNESP]
Rocha, Ramon S.
Pimentel, Tatiana C.
Esmerino, Erick A.
Freitas, Mônica Q.
Santillo, Antonella
Silva, Marcia Cristina
Barbisan, Luis F.
Cruz, Adriano G.
Albenzio, Marzia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Federal Fluminense University (UFF)
Universidade Estadual Paulista (Unesp)
Federal Institute of Rio de Janeiro (IFRJ)
Federal Institute of Paraná (IFPR)
Rural Federal University of Rio de Janeiro (UFRRJ)
University of Foggia (UNIFG)
dc.contributor.author.fl_str_mv Balthazar, Celso F.
de Moura, Nelci A. [UNESP]
Romualdo, Guilherme R. [UNESP]
Rocha, Ramon S.
Pimentel, Tatiana C.
Esmerino, Erick A.
Freitas, Mônica Q.
Santillo, Antonella
Silva, Marcia Cristina
Barbisan, Luis F.
Cruz, Adriano G.
Albenzio, Marzia
dc.subject.por.fl_str_mv azoxymethane
colon carcinogenesis
sheep dairy food
synbiotic
topic azoxymethane
colon carcinogenesis
sheep dairy food
synbiotic
description Sheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:29:10Z
2021-06-25T10:29:10Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3168/jds.2020-19979
Journal of Dairy Science.
1525-3198
0022-0302
http://hdl.handle.net/11449/206257
10.3168/jds.2020-19979
2-s2.0-85104947682
url http://dx.doi.org/10.3168/jds.2020-19979
http://hdl.handle.net/11449/206257
identifier_str_mv Journal of Dairy Science.
1525-3198
0022-0302
10.3168/jds.2020-19979
2-s2.0-85104947682
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Dairy Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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