Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0074268 http://hdl.handle.net/11449/76566 |
Resumo: | Background:Lutzomyia longipalpis (Diptera: Psychodidae) is the major vector of Leishmania (Leishmania) infantum and thus plays a crucial role in the epidemiology of American visceral leishmaniasis (AVL). This vector is the best studied species of sand fly in the Neotropical region. Many studies claim that this vector is in fact a species complex; however there is still no consensus regarding the number of species that belong into this complex or the geographical distribution of sibling species. The aim of the present study was to analyze the genetic relationships within Lu. longipalpis populations in the state of Mato Grosso do Sul (MS), Brazil.Methodology/Principal Findings:We collected 30 Lu. longipalpis (15 females and 15 males) from five localities (Campo Grande, Três Lagoas, Aquidauana, Miranda and Bonito) and 30 Lu. Cruzi from Corumbá, totaling 180 sandflies from MS, and 30 Lu. longipalpis from Estrela de Alagoas, state of Alagoas (AL), Northeast Brazil. We show that eight previously described microsatellite loci were sufficient in distinguishing Lu. longipalpis from Lu. Cruzi, which is a closely related species, and in differentiating between Lu. longipalpis collected in MS versus Estrela de Alagoas. Analyses of the genotypes revealed introgression between sympatric Lu. longipalpis and Lu. Cruzi.Conclusions/Significance:Our findings support the hypothesis of cryptic species within the Lu. longipalpis complex. Furthermore, our data revealed introgression between Lu. longipalpis and Lu. cruzi. This phenomenon should be further investigated to determine the level and incidence of hybridization between these two species. We also demonstrated that microsatellite markers are a powerful tool for differentiating sand fly populations and species. The present study has elucidated the population structure of Lu. longipalpis in MS and, by extension, the Neotropical Lu. longipalpis complex itself. © 2013 Santos et al. |
id |
UNSP_31ae3ffc8130f305eba6d07e7cdb7559 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/76566 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite MarkersBackground:Lutzomyia longipalpis (Diptera: Psychodidae) is the major vector of Leishmania (Leishmania) infantum and thus plays a crucial role in the epidemiology of American visceral leishmaniasis (AVL). This vector is the best studied species of sand fly in the Neotropical region. Many studies claim that this vector is in fact a species complex; however there is still no consensus regarding the number of species that belong into this complex or the geographical distribution of sibling species. The aim of the present study was to analyze the genetic relationships within Lu. longipalpis populations in the state of Mato Grosso do Sul (MS), Brazil.Methodology/Principal Findings:We collected 30 Lu. longipalpis (15 females and 15 males) from five localities (Campo Grande, Três Lagoas, Aquidauana, Miranda and Bonito) and 30 Lu. Cruzi from Corumbá, totaling 180 sandflies from MS, and 30 Lu. longipalpis from Estrela de Alagoas, state of Alagoas (AL), Northeast Brazil. We show that eight previously described microsatellite loci were sufficient in distinguishing Lu. longipalpis from Lu. Cruzi, which is a closely related species, and in differentiating between Lu. longipalpis collected in MS versus Estrela de Alagoas. Analyses of the genotypes revealed introgression between sympatric Lu. longipalpis and Lu. Cruzi.Conclusions/Significance:Our findings support the hypothesis of cryptic species within the Lu. longipalpis complex. Furthermore, our data revealed introgression between Lu. longipalpis and Lu. cruzi. This phenomenon should be further investigated to determine the level and incidence of hybridization between these two species. We also demonstrated that microsatellite markers are a powerful tool for differentiating sand fly populations and species. The present study has elucidated the population structure of Lu. longipalpis in MS and, by extension, the Neotropical Lu. longipalpis complex itself. © 2013 Santos et al.Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do SulDepartamento de Parasitologia Universidade Estadual Paulista Júlio de Mesquita Filho Unesp, Botucatu, São PauloReferencia Nacional e Internacional de Flebotomíneos Centro de Pesquisas René Rachou CCPQRR-FIOCRUZ, Belo Horizonte, Minas GeraisLaboratório de Imunologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do SulLaboratório de Patologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do SulLaboratório de Doenças Parasitárias Instituto Oswaldo Cruz FIOCRUZ, Rio de Janeiro, Rio de JaneiroLaboratório de Parasitologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do SulDepartamento de Parasitologia Universidade Estadual Paulista Júlio de Mesquita Filho Unesp, Botucatu, São PauloUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)CCPQRR-FIOCRUZFIOCRUZSantos, Mirella F. C.Ribolla, Paulo E. M. [UNESP]Alonso, Diego P. [UNESP]Andrade-Filho, José D.Casaril, Aline E.Ferreira, Alda M. T.Fernandes, Carlos E. S.Brazil, Reginaldo P.Oliveira, Alessandra G.2014-05-27T11:30:41Z2014-05-27T11:30:41Z2013-09-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0074268PLoS ONE, v. 8, n. 9, 2013.1932-6203http://hdl.handle.net/11449/7656610.1371/journal.pone.0074268WOS:0003244940001112-s2.0-848841855112-s2.0-84884185511.pdf35771497484568800000-0001-8735-6090Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-10-12T06:03:01Zoai:repositorio.unesp.br:11449/76566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:39:42.969620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
title |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
spellingShingle |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers Santos, Mirella F. C. |
title_short |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
title_full |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
title_fullStr |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
title_full_unstemmed |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
title_sort |
Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers |
author |
Santos, Mirella F. C. |
author_facet |
Santos, Mirella F. C. Ribolla, Paulo E. M. [UNESP] Alonso, Diego P. [UNESP] Andrade-Filho, José D. Casaril, Aline E. Ferreira, Alda M. T. Fernandes, Carlos E. S. Brazil, Reginaldo P. Oliveira, Alessandra G. |
author_role |
author |
author2 |
Ribolla, Paulo E. M. [UNESP] Alonso, Diego P. [UNESP] Andrade-Filho, José D. Casaril, Aline E. Ferreira, Alda M. T. Fernandes, Carlos E. S. Brazil, Reginaldo P. Oliveira, Alessandra G. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) CCPQRR-FIOCRUZ FIOCRUZ |
dc.contributor.author.fl_str_mv |
Santos, Mirella F. C. Ribolla, Paulo E. M. [UNESP] Alonso, Diego P. [UNESP] Andrade-Filho, José D. Casaril, Aline E. Ferreira, Alda M. T. Fernandes, Carlos E. S. Brazil, Reginaldo P. Oliveira, Alessandra G. |
description |
Background:Lutzomyia longipalpis (Diptera: Psychodidae) is the major vector of Leishmania (Leishmania) infantum and thus plays a crucial role in the epidemiology of American visceral leishmaniasis (AVL). This vector is the best studied species of sand fly in the Neotropical region. Many studies claim that this vector is in fact a species complex; however there is still no consensus regarding the number of species that belong into this complex or the geographical distribution of sibling species. The aim of the present study was to analyze the genetic relationships within Lu. longipalpis populations in the state of Mato Grosso do Sul (MS), Brazil.Methodology/Principal Findings:We collected 30 Lu. longipalpis (15 females and 15 males) from five localities (Campo Grande, Três Lagoas, Aquidauana, Miranda and Bonito) and 30 Lu. Cruzi from Corumbá, totaling 180 sandflies from MS, and 30 Lu. longipalpis from Estrela de Alagoas, state of Alagoas (AL), Northeast Brazil. We show that eight previously described microsatellite loci were sufficient in distinguishing Lu. longipalpis from Lu. Cruzi, which is a closely related species, and in differentiating between Lu. longipalpis collected in MS versus Estrela de Alagoas. Analyses of the genotypes revealed introgression between sympatric Lu. longipalpis and Lu. Cruzi.Conclusions/Significance:Our findings support the hypothesis of cryptic species within the Lu. longipalpis complex. Furthermore, our data revealed introgression between Lu. longipalpis and Lu. cruzi. This phenomenon should be further investigated to determine the level and incidence of hybridization between these two species. We also demonstrated that microsatellite markers are a powerful tool for differentiating sand fly populations and species. The present study has elucidated the population structure of Lu. longipalpis in MS and, by extension, the Neotropical Lu. longipalpis complex itself. © 2013 Santos et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-16 2014-05-27T11:30:41Z 2014-05-27T11:30:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0074268 PLoS ONE, v. 8, n. 9, 2013. 1932-6203 http://hdl.handle.net/11449/76566 10.1371/journal.pone.0074268 WOS:000324494000111 2-s2.0-84884185511 2-s2.0-84884185511.pdf 3577149748456880 0000-0001-8735-6090 |
url |
http://dx.doi.org/10.1371/journal.pone.0074268 http://hdl.handle.net/11449/76566 |
identifier_str_mv |
PLoS ONE, v. 8, n. 9, 2013. 1932-6203 10.1371/journal.pone.0074268 WOS:000324494000111 2-s2.0-84884185511 2-s2.0-84884185511.pdf 3577149748456880 0000-0001-8735-6090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128397089439744 |