Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10493-021-00591-w http://hdl.handle.net/11449/205947 |
Resumo: | Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks. |
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Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory moleculesEctoparasite-host relationshipsNitric oxideSalivaTicksRhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biology Institute of Biosciences São Paulo State University – UNESPDepartment of Genetics Evolution and Bioagents Institute of Biology University of Campinas – UNICAMPDepartment of Biology Institute of Biosciences São Paulo State University – UNESPFAPESP: 2014/02843-8FAPESP: 2015/20745-6FAPESP: 2018/2999-9FAPESP: 2019/02831-3CNPq: 300625/2012-0Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Pereira, Melissa Carolina [UNESP]Nodari, Elen Fernanda [UNESP]de Abreu, Marina Rodrigues [UNESP]Paiatto, Lisiery NegriniSimioni, Patrícia UcelliCamargo-Mathias, Maria Izabel [UNESP]2021-06-25T10:24:08Z2021-06-25T10:24:08Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-398http://dx.doi.org/10.1007/s10493-021-00591-wExperimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021.1572-97020168-8162http://hdl.handle.net/11449/20594710.1007/s10493-021-00591-w2-s2.0-85101456658Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental and Applied Acarologyinfo:eu-repo/semantics/openAccess2021-10-22T20:11:14Zoai:repositorio.unesp.br:11449/205947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:43:51.215472Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
title |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
spellingShingle |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules Pereira, Melissa Carolina [UNESP] Ectoparasite-host relationships Nitric oxide Saliva Ticks |
title_short |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
title_full |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
title_fullStr |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
title_full_unstemmed |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
title_sort |
Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules |
author |
Pereira, Melissa Carolina [UNESP] |
author_facet |
Pereira, Melissa Carolina [UNESP] Nodari, Elen Fernanda [UNESP] de Abreu, Marina Rodrigues [UNESP] Paiatto, Lisiery Negrini Simioni, Patrícia Ucelli Camargo-Mathias, Maria Izabel [UNESP] |
author_role |
author |
author2 |
Nodari, Elen Fernanda [UNESP] de Abreu, Marina Rodrigues [UNESP] Paiatto, Lisiery Negrini Simioni, Patrícia Ucelli Camargo-Mathias, Maria Izabel [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Pereira, Melissa Carolina [UNESP] Nodari, Elen Fernanda [UNESP] de Abreu, Marina Rodrigues [UNESP] Paiatto, Lisiery Negrini Simioni, Patrícia Ucelli Camargo-Mathias, Maria Izabel [UNESP] |
dc.subject.por.fl_str_mv |
Ectoparasite-host relationships Nitric oxide Saliva Ticks |
topic |
Ectoparasite-host relationships Nitric oxide Saliva Ticks |
description |
Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:24:08Z 2021-06-25T10:24:08Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10493-021-00591-w Experimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021. 1572-9702 0168-8162 http://hdl.handle.net/11449/205947 10.1007/s10493-021-00591-w 2-s2.0-85101456658 |
url |
http://dx.doi.org/10.1007/s10493-021-00591-w http://hdl.handle.net/11449/205947 |
identifier_str_mv |
Experimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021. 1572-9702 0168-8162 10.1007/s10493-021-00591-w 2-s2.0-85101456658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental and Applied Acarology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
387-398 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129239382228992 |