Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules

Detalhes bibliográficos
Autor(a) principal: Pereira, Melissa Carolina [UNESP]
Data de Publicação: 2021
Outros Autores: Nodari, Elen Fernanda [UNESP], de Abreu, Marina Rodrigues [UNESP], Paiatto, Lisiery Negrini, Simioni, Patrícia Ucelli, Camargo-Mathias, Maria Izabel [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10493-021-00591-w
http://hdl.handle.net/11449/205947
Resumo: Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks.
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spelling Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory moleculesEctoparasite-host relationshipsNitric oxideSalivaTicksRhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biology Institute of Biosciences São Paulo State University – UNESPDepartment of Genetics Evolution and Bioagents Institute of Biology University of Campinas – UNICAMPDepartment of Biology Institute of Biosciences São Paulo State University – UNESPFAPESP: 2014/02843-8FAPESP: 2015/20745-6FAPESP: 2018/2999-9FAPESP: 2019/02831-3CNPq: 300625/2012-0Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Pereira, Melissa Carolina [UNESP]Nodari, Elen Fernanda [UNESP]de Abreu, Marina Rodrigues [UNESP]Paiatto, Lisiery NegriniSimioni, Patrícia UcelliCamargo-Mathias, Maria Izabel [UNESP]2021-06-25T10:24:08Z2021-06-25T10:24:08Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-398http://dx.doi.org/10.1007/s10493-021-00591-wExperimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021.1572-97020168-8162http://hdl.handle.net/11449/20594710.1007/s10493-021-00591-w2-s2.0-85101456658Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental and Applied Acarologyinfo:eu-repo/semantics/openAccess2021-10-22T20:11:14Zoai:repositorio.unesp.br:11449/205947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:43:51.215472Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
title Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
spellingShingle Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
Pereira, Melissa Carolina [UNESP]
Ectoparasite-host relationships
Nitric oxide
Saliva
Ticks
title_short Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
title_full Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
title_fullStr Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
title_full_unstemmed Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
title_sort Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
author Pereira, Melissa Carolina [UNESP]
author_facet Pereira, Melissa Carolina [UNESP]
Nodari, Elen Fernanda [UNESP]
de Abreu, Marina Rodrigues [UNESP]
Paiatto, Lisiery Negrini
Simioni, Patrícia Ucelli
Camargo-Mathias, Maria Izabel [UNESP]
author_role author
author2 Nodari, Elen Fernanda [UNESP]
de Abreu, Marina Rodrigues [UNESP]
Paiatto, Lisiery Negrini
Simioni, Patrícia Ucelli
Camargo-Mathias, Maria Izabel [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Pereira, Melissa Carolina [UNESP]
Nodari, Elen Fernanda [UNESP]
de Abreu, Marina Rodrigues [UNESP]
Paiatto, Lisiery Negrini
Simioni, Patrícia Ucelli
Camargo-Mathias, Maria Izabel [UNESP]
dc.subject.por.fl_str_mv Ectoparasite-host relationships
Nitric oxide
Saliva
Ticks
topic Ectoparasite-host relationships
Nitric oxide
Saliva
Ticks
description Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:24:08Z
2021-06-25T10:24:08Z
2021-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10493-021-00591-w
Experimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021.
1572-9702
0168-8162
http://hdl.handle.net/11449/205947
10.1007/s10493-021-00591-w
2-s2.0-85101456658
url http://dx.doi.org/10.1007/s10493-021-00591-w
http://hdl.handle.net/11449/205947
identifier_str_mv Experimental and Applied Acarology, v. 83, n. 3, p. 387-398, 2021.
1572-9702
0168-8162
10.1007/s10493-021-00591-w
2-s2.0-85101456658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental and Applied Acarology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 387-398
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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