Detecção molecular de espécies de Anaplasma em cães na Colômbia

Detalhes bibliográficos
Autor(a) principal: Vargas-Hernandez, Giovanni [UNESP]
Data de Publicação: 2016
Outros Autores: André, Marcos Rogério [UNESP], Cendales, Diana Maria, de Sousa, Keyla Carstens Marques [UNESP], Gonçalves, Luiz Ricardo [UNESP], Rondelli, Mariana Cristina Hoeppner, Machado, Rosangela Zacarias [UNESP], Tinucci-Costa, Mirela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612016066
http://hdl.handle.net/11449/178510
Resumo: Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors’ knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.
id UNSP_31fc83629341fb86e53d64d3a3019d4a
oai_identifier_str oai:repositorio.unesp.br:11449/178510
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Detecção molecular de espécies de Anaplasma em cães na ColômbiaMolecular detection of Anaplasma species in dogs in ColombiaAnaplasma platysAnaplasma sppColombiaDogsAnaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors’ knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Salud Animal Facultad de Medicina Veterinaria y de Zootecnia Universidad Nacional de ColombiaFaculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPInstituto Federal de Educação Ciência e Tecnologia do Sul de Minas Gerais-IFSULDEMINASFaculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPUniversidad Nacional de ColombiaUniversidade Estadual Paulista (Unesp)Ciência e Tecnologia do Sul de Minas Gerais-IFSULDEMINASVargas-Hernandez, Giovanni [UNESP]André, Marcos Rogério [UNESP]Cendales, Diana Mariade Sousa, Keyla Carstens Marques [UNESP]Gonçalves, Luiz Ricardo [UNESP]Rondelli, Mariana Cristina HoeppnerMachado, Rosangela Zacarias [UNESP]Tinucci-Costa, Mirela [UNESP]2018-12-11T17:30:41Z2018-12-11T17:30:41Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article459-464application/pdfhttp://dx.doi.org/10.1590/S1984-29612016066Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 459-464, 2016.0103-846Xhttp://hdl.handle.net/11449/17851010.1590/S1984-29612016066S1984-296120160004004592-s2.0-85006741515S1984-29612016000400459.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:27Zoai:repositorio.unesp.br:11449/178510Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:11:00.156282Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detecção molecular de espécies de Anaplasma em cães na Colômbia
Molecular detection of Anaplasma species in dogs in Colombia
title Detecção molecular de espécies de Anaplasma em cães na Colômbia
spellingShingle Detecção molecular de espécies de Anaplasma em cães na Colômbia
Vargas-Hernandez, Giovanni [UNESP]
Anaplasma platys
Anaplasma spp
Colombia
Dogs
title_short Detecção molecular de espécies de Anaplasma em cães na Colômbia
title_full Detecção molecular de espécies de Anaplasma em cães na Colômbia
title_fullStr Detecção molecular de espécies de Anaplasma em cães na Colômbia
title_full_unstemmed Detecção molecular de espécies de Anaplasma em cães na Colômbia
title_sort Detecção molecular de espécies de Anaplasma em cães na Colômbia
author Vargas-Hernandez, Giovanni [UNESP]
author_facet Vargas-Hernandez, Giovanni [UNESP]
André, Marcos Rogério [UNESP]
Cendales, Diana Maria
de Sousa, Keyla Carstens Marques [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Rondelli, Mariana Cristina Hoeppner
Machado, Rosangela Zacarias [UNESP]
Tinucci-Costa, Mirela [UNESP]
author_role author
author2 André, Marcos Rogério [UNESP]
Cendales, Diana Maria
de Sousa, Keyla Carstens Marques [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Rondelli, Mariana Cristina Hoeppner
Machado, Rosangela Zacarias [UNESP]
Tinucci-Costa, Mirela [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Nacional de Colombia
Universidade Estadual Paulista (Unesp)
Ciência e Tecnologia do Sul de Minas Gerais-IFSULDEMINAS
dc.contributor.author.fl_str_mv Vargas-Hernandez, Giovanni [UNESP]
André, Marcos Rogério [UNESP]
Cendales, Diana Maria
de Sousa, Keyla Carstens Marques [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Rondelli, Mariana Cristina Hoeppner
Machado, Rosangela Zacarias [UNESP]
Tinucci-Costa, Mirela [UNESP]
dc.subject.por.fl_str_mv Anaplasma platys
Anaplasma spp
Colombia
Dogs
topic Anaplasma platys
Anaplasma spp
Colombia
Dogs
description Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors’ knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2018-12-11T17:30:41Z
2018-12-11T17:30:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612016066
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 459-464, 2016.
0103-846X
http://hdl.handle.net/11449/178510
10.1590/S1984-29612016066
S1984-29612016000400459
2-s2.0-85006741515
S1984-29612016000400459.pdf
9139899895580513
url http://dx.doi.org/10.1590/S1984-29612016066
http://hdl.handle.net/11449/178510
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 459-464, 2016.
0103-846X
10.1590/S1984-29612016066
S1984-29612016000400459
2-s2.0-85006741515
S1984-29612016000400459.pdf
9139899895580513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 459-464
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128229524897792