Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino

Detalhes bibliográficos
Autor(a) principal: Mello-Peixoto, Erika Cosendey Toledo de
Data de Publicação: 2014
Outros Autores: Moreira, Giovanna Melatti Bermal, Matsumoto, Leopoldo Sussumu, Silva, Regildo Márcio Gonçalves da [UNESP], Domingues, Paulo Francisco [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.aba-agroecologia.org.br/revistas/index.php/cad/article/view/15492
http://hdl.handle.net/11449/123432
Resumo: Mastitis is characterized by mammary inflammation in response to bacterial infection. The aim of this study was to investigate the antibacterial activity of pomegranate extract on Staphylococcus spp. The milk samples were plated and the colonies were adjusted to 1.0 x106 UFC.mL-1 to Standard no. 6 McFarland scale. The sensitivity of the isolates was determined by disk diffusion and the minimum inhibitory concentration was determined by the halo of inhibition greater than 15 mm. The extracts were evaluated by ANOVA and Tukey test 5%. Additionally it was evaluated antioxidant activity, levels of total phenols and flavonoids. The bacterial growth was inhibited and the antioxidant activity was observed from 50 μg.mL-1. However, it was not possible to correlate this activity to the levels of total phenols and flavonoids, probably other substances were responsible for antioxidant activity. It was concluded that the hydroalcoholic extract of pomegranate has antimicrobial activity against Staphylococcus spp.
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spelling Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovinoMastitePlantas medicinaisProdução orgânicaRomãMastitesMedicinal plantsOrganic productionPomegranateMastitis is characterized by mammary inflammation in response to bacterial infection. The aim of this study was to investigate the antibacterial activity of pomegranate extract on Staphylococcus spp. The milk samples were plated and the colonies were adjusted to 1.0 x106 UFC.mL-1 to Standard no. 6 McFarland scale. The sensitivity of the isolates was determined by disk diffusion and the minimum inhibitory concentration was determined by the halo of inhibition greater than 15 mm. The extracts were evaluated by ANOVA and Tukey test 5%. Additionally it was evaluated antioxidant activity, levels of total phenols and flavonoids. The bacterial growth was inhibited and the antioxidant activity was observed from 50 μg.mL-1. However, it was not possible to correlate this activity to the levels of total phenols and flavonoids, probably other substances were responsible for antioxidant activity. It was concluded that the hydroalcoholic extract of pomegranate has antimicrobial activity against Staphylococcus spp.Mastite é caraterizada por inflamação mamária em resposta à infecção bacteriana. Objetivou-se verificar ação antibacteriana do extrato de romã sobre sobre Staphylococcus spp. Amostras de leite foram semeadas e as colônias ajustadas a 1,0x106 UFC.mL-1 ao padrão nº 6 da escala de MacFarland. A sensibilidade dos isolados foi determinada por difusão em discos e a concentração inibitória mínima foi determinada pelo halo de inibição superior a 15 mm. Os extratos foram avaliados pelo método ANOVA e teste de Tukey 5%. Adicionalmente avaliou-se atividade antioxidante, teores de fenóis e flavonoides totais. O crescimento bacteriano foi inibido e a ação antioxidante foi verificada a partir de 50 μg.mL-1. Porém, não foi possível correlacionar esta atividade aos teores de fenóis e flavonoides totais, provavelmente outras substâncias presentes foram responsáveis pela ação antioxidante. Conclui-se que o extrato hidroalcoólico de romã apresenta atividade antimicrobiana contra Staphylococcus spp.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências e Letras de Assis, Assis, Avenida Dom Antonio, 2100, Vila Universitária, CEP 19806-900, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências e Letras de Assis, Assis, Avenida Dom Antonio, 2100, Vila Universitária, CEP 19806-900, SP, BrasilUniversidade Estadual do Norte do Paraná - UENP, emellopeixoto@uenp.edu.brUniversidade Estadual do Norte do Paraná- UENP, gimelatti@hotmail.comUniversidade Estadual do Norte do Paraná Campus Bandeirantes - UENP, leopoldo@uenp.edu.brUniversidade Estadual Paulista Júlio Mesquita Filho, Campus Assis – UNESP, regildos@yahoo.com.brUniversidade Estadual Paulista Júlio Mesquita Filho, Campus Botucatu – UNESP, domingues@fmvz.unesp.brUniversidade Estadual Paulista (Unesp)Mello-Peixoto, Erika Cosendey Toledo deMoreira, Giovanna Melatti BermalMatsumoto, Leopoldo SussumuSilva, Regildo Márcio Gonçalves da [UNESP]Domingues, Paulo Francisco [UNESP]2015-05-15T13:30:11Z2015-05-15T13:30:11Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://www.aba-agroecologia.org.br/revistas/index.php/cad/article/view/15492Cadernos de Agroecologia, v. 9, n. 1, p. 1-5, 2014.2236-7934http://hdl.handle.net/11449/123432ISSN02236-7934-2014-09-01-01-05.pdf528532436116257833423137958829540000-0002-0300-0247Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinária Brasileirainfo:eu-repo/semantics/openAccess2023-11-03T06:08:54Zoai:repositorio.unesp.br:11449/123432Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:48:00.634107Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
title Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
spellingShingle Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
Mello-Peixoto, Erika Cosendey Toledo de
Mastite
Plantas medicinais
Produção orgânica
Romã
Mastites
Medicinal plants
Organic production
Pomegranate
title_short Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
title_full Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
title_fullStr Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
title_full_unstemmed Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
title_sort Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino
author Mello-Peixoto, Erika Cosendey Toledo de
author_facet Mello-Peixoto, Erika Cosendey Toledo de
Moreira, Giovanna Melatti Bermal
Matsumoto, Leopoldo Sussumu
Silva, Regildo Márcio Gonçalves da [UNESP]
Domingues, Paulo Francisco [UNESP]
author_role author
author2 Moreira, Giovanna Melatti Bermal
Matsumoto, Leopoldo Sussumu
Silva, Regildo Márcio Gonçalves da [UNESP]
Domingues, Paulo Francisco [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mello-Peixoto, Erika Cosendey Toledo de
Moreira, Giovanna Melatti Bermal
Matsumoto, Leopoldo Sussumu
Silva, Regildo Márcio Gonçalves da [UNESP]
Domingues, Paulo Francisco [UNESP]
dc.subject.por.fl_str_mv Mastite
Plantas medicinais
Produção orgânica
Romã
Mastites
Medicinal plants
Organic production
Pomegranate
topic Mastite
Plantas medicinais
Produção orgânica
Romã
Mastites
Medicinal plants
Organic production
Pomegranate
description Mastitis is characterized by mammary inflammation in response to bacterial infection. The aim of this study was to investigate the antibacterial activity of pomegranate extract on Staphylococcus spp. The milk samples were plated and the colonies were adjusted to 1.0 x106 UFC.mL-1 to Standard no. 6 McFarland scale. The sensitivity of the isolates was determined by disk diffusion and the minimum inhibitory concentration was determined by the halo of inhibition greater than 15 mm. The extracts were evaluated by ANOVA and Tukey test 5%. Additionally it was evaluated antioxidant activity, levels of total phenols and flavonoids. The bacterial growth was inhibited and the antioxidant activity was observed from 50 μg.mL-1. However, it was not possible to correlate this activity to the levels of total phenols and flavonoids, probably other substances were responsible for antioxidant activity. It was concluded that the hydroalcoholic extract of pomegranate has antimicrobial activity against Staphylococcus spp.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-05-15T13:30:11Z
2015-05-15T13:30:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.aba-agroecologia.org.br/revistas/index.php/cad/article/view/15492
Cadernos de Agroecologia, v. 9, n. 1, p. 1-5, 2014.
2236-7934
http://hdl.handle.net/11449/123432
ISSN02236-7934-2014-09-01-01-05.pdf
5285324361162578
3342313795882954
0000-0002-0300-0247
url http://www.aba-agroecologia.org.br/revistas/index.php/cad/article/view/15492
http://hdl.handle.net/11449/123432
identifier_str_mv Cadernos de Agroecologia, v. 9, n. 1, p. 1-5, 2014.
2236-7934
ISSN02236-7934-2014-09-01-01-05.pdf
5285324361162578
3342313795882954
0000-0002-0300-0247
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Pesquisa Veterinária Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-5
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128703117393920

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