Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela

Detalhes bibliográficos
Autor(a) principal: Mariguela, T. C. [UNESP]
Data de Publicação: 2010
Outros Autores: Nirchio, M., Ron, E., Gaviria, J. I., Foresti, Fausto [UNESP], Oliveira, C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.pensoft.net/journals/compcytogen/article/1097/
http://hdl.handle.net/11449/18761
Resumo: A cytogenetic analysis by conventional Giemsa staining, silver staining, C-banding, and fluorescence in situ hybridization (FISH) was carried out on Brycon amazonicus from Caicara del Orinoco, Venezuela. The karyotype of this species is characterized by the presence of 2n = 50 chromosomes, a karyotypic formula 22m+14sm+14st, and a fundamental number of 100 chromosomal arms. Nucleolar organizer regions (NORs) and 18S rDNA genes are located in the terminal regions of the long arms of the second pair of subtelocentric chromosomes, corresponding to pair 13. C-banding revealed heterochromatin distribution in only seven chromosome pairs. The largest metacentric pair (number 1) possesses a paracentromeric block equilocally distributed in both chromosome arms, whereas in pairs 12 to 17 positive C band blocks were located in the paracentromeric region of the long arm, close to the centromere. Analysis performed with 5S rDNA gene revealed a terminal site located on the short arm of one small submetacentric chromosome (pair 15) corroborating previous studies with this repetitive gene showing an apparent conservation of 5S rDNA in the genome of these fish species. The data obtained in this study reinforce the chromosomal conservativeness in the species of the genus Brycon, related not only to the macro-chromosomal structure (diploid number, karyotypic formula, and fundamental number), but also to the repeated DNAs, such as heterochromatic regions and ribosomal DNAs. These data will contribute to a better understanding of chromosomal evolution in both Brycon and Characidae fishes.
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spelling Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, VenezuelaCharacidaeC-bandingkaryotypeNORs18S rDNA5S rDNAA cytogenetic analysis by conventional Giemsa staining, silver staining, C-banding, and fluorescence in situ hybridization (FISH) was carried out on Brycon amazonicus from Caicara del Orinoco, Venezuela. The karyotype of this species is characterized by the presence of 2n = 50 chromosomes, a karyotypic formula 22m+14sm+14st, and a fundamental number of 100 chromosomal arms. Nucleolar organizer regions (NORs) and 18S rDNA genes are located in the terminal regions of the long arms of the second pair of subtelocentric chromosomes, corresponding to pair 13. C-banding revealed heterochromatin distribution in only seven chromosome pairs. The largest metacentric pair (number 1) possesses a paracentromeric block equilocally distributed in both chromosome arms, whereas in pairs 12 to 17 positive C band blocks were located in the paracentromeric region of the long arm, close to the centromere. Analysis performed with 5S rDNA gene revealed a terminal site located on the short arm of one small submetacentric chromosome (pair 15) corroborating previous studies with this repetitive gene showing an apparent conservation of 5S rDNA in the genome of these fish species. The data obtained in this study reinforce the chromosomal conservativeness in the species of the genus Brycon, related not only to the macro-chromosomal structure (diploid number, karyotypic formula, and fundamental number), but also to the repeated DNAs, such as heterochromatic regions and ribosomal DNAs. These data will contribute to a better understanding of chromosomal evolution in both Brycon and Characidae fishes.Consejo de InvestigacionUniversidad de Oriente, VenezuelaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista UNESP, Dept Morfol, Lab Biol & Genet Peixes, BR-18618970 Botucatu, SP, BrazilUniv Oriente, Escuela Ciencias Aplicadas Mar, Nucleo Nueva Esparta, Porlamar, Margarita, VenezuelaUniv Estadual Paulista UNESP, Dept Morfol, Lab Biol & Genet Peixes, BR-18618970 Botucatu, SP, BrazilZoological InstUniversidade Estadual Paulista (Unesp)Univ OrienteMariguela, T. C. [UNESP]Nirchio, M.Ron, E.Gaviria, J. I.Foresti, Fausto [UNESP]Oliveira, C. [UNESP]2014-05-20T13:52:33Z2014-05-20T13:52:33Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article185-193application/pdfhttp://www.pensoft.net/journals/compcytogen/article/1097/Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 4, n. 2, p. 185-193, 2010.1993-0771http://hdl.handle.net/11449/1876110.3897/compcytogen.v4i2.49WOS:000288103800009WOS000288103800009.pdf0804793944846367Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogenetics1.3190,431info:eu-repo/semantics/openAccess2023-12-05T06:14:37Zoai:repositorio.unesp.br:11449/18761Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:44.424151Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
title Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
spellingShingle Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
Mariguela, T. C. [UNESP]
Characidae
C-banding
karyotype
NORs
18S rDNA
5S rDNA
title_short Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
title_full Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
title_fullStr Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
title_full_unstemmed Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
title_sort Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela
author Mariguela, T. C. [UNESP]
author_facet Mariguela, T. C. [UNESP]
Nirchio, M.
Ron, E.
Gaviria, J. I.
Foresti, Fausto [UNESP]
Oliveira, C. [UNESP]
author_role author
author2 Nirchio, M.
Ron, E.
Gaviria, J. I.
Foresti, Fausto [UNESP]
Oliveira, C. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Oriente
dc.contributor.author.fl_str_mv Mariguela, T. C. [UNESP]
Nirchio, M.
Ron, E.
Gaviria, J. I.
Foresti, Fausto [UNESP]
Oliveira, C. [UNESP]
dc.subject.por.fl_str_mv Characidae
C-banding
karyotype
NORs
18S rDNA
5S rDNA
topic Characidae
C-banding
karyotype
NORs
18S rDNA
5S rDNA
description A cytogenetic analysis by conventional Giemsa staining, silver staining, C-banding, and fluorescence in situ hybridization (FISH) was carried out on Brycon amazonicus from Caicara del Orinoco, Venezuela. The karyotype of this species is characterized by the presence of 2n = 50 chromosomes, a karyotypic formula 22m+14sm+14st, and a fundamental number of 100 chromosomal arms. Nucleolar organizer regions (NORs) and 18S rDNA genes are located in the terminal regions of the long arms of the second pair of subtelocentric chromosomes, corresponding to pair 13. C-banding revealed heterochromatin distribution in only seven chromosome pairs. The largest metacentric pair (number 1) possesses a paracentromeric block equilocally distributed in both chromosome arms, whereas in pairs 12 to 17 positive C band blocks were located in the paracentromeric region of the long arm, close to the centromere. Analysis performed with 5S rDNA gene revealed a terminal site located on the short arm of one small submetacentric chromosome (pair 15) corroborating previous studies with this repetitive gene showing an apparent conservation of 5S rDNA in the genome of these fish species. The data obtained in this study reinforce the chromosomal conservativeness in the species of the genus Brycon, related not only to the macro-chromosomal structure (diploid number, karyotypic formula, and fundamental number), but also to the repeated DNAs, such as heterochromatic regions and ribosomal DNAs. These data will contribute to a better understanding of chromosomal evolution in both Brycon and Characidae fishes.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2014-05-20T13:52:33Z
2014-05-20T13:52:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.pensoft.net/journals/compcytogen/article/1097/
Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 4, n. 2, p. 185-193, 2010.
1993-0771
http://hdl.handle.net/11449/18761
10.3897/compcytogen.v4i2.49
WOS:000288103800009
WOS000288103800009.pdf
0804793944846367
url http://www.pensoft.net/journals/compcytogen/article/1097/
http://hdl.handle.net/11449/18761
identifier_str_mv Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 4, n. 2, p. 185-193, 2010.
1993-0771
10.3897/compcytogen.v4i2.49
WOS:000288103800009
WOS000288103800009.pdf
0804793944846367
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Comparative Cytogenetics
1.319
0,431
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 185-193
application/pdf
dc.publisher.none.fl_str_mv Zoological Inst
publisher.none.fl_str_mv Zoological Inst
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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