Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

• Autor(a) principal: Farac, Roberta Vieira [UNESP]
• Data de Publicação: 2013
• Outros Autores: Pizzolitto, Antonio Carlos [UNESP], Tanomaru, Juliane Maria Guerreiro [UNESP], Morgental, Renata Dornelles, Lima, Regina Karla de Pontes [UNESP], Bonetti-Filho, Idomeo [UNESP]
• Tipo de documento: Artigo
• Idioma: eng; por
• Título da fonte: Repositório Institucional da UNESP
• DOI: 10.1590/0103-6440201301992
• Texto Completo: http://dx.doi.org/10.1590/0103-6440201301992; http://hdl.handle.net/11449/74736
• topic: Calcium hydroxide; Endodontics; Enterococcus faecalis; Ozone; Root canal preparation

This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.