Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri

Detalhes bibliográficos
Autor(a) principal: de Gregorio, Lara S. [UNESP]
Data de Publicação: 2016
Outros Autores: Franco-Belussi, Lilian [UNESP], Gomes, Fernando R., de Oliveira, Classius [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquatox.2016.04.022
http://hdl.handle.net/11449/168652
Resumo: Water contamination is one of the factors influencing the decline of amphibians. Flutamide is an antiandrogenic medicine that occurs as water contaminant. This compound especially affects the reproductive organs, but it can also show hepatotoxic effects. The Bufonidae family has a peculiar organ named Bidder's organ, considered by some authors as a rudimentary ovary, but capable to respond to some external stimuli. This study investigated flutamide effects on testes and Bidder's organ germ cells, liver pigmentation, and sexual hormones levels in Rhinella schneideri males. We randomly divided 15 males in three groups (N = 5): two groups were injected with flutamide, at 1 and 5 mg/kg, while the control group received only mineral oil, for 7 days. After euthanasia, blood samples were collected and the organs were sent to histological routine. In the testes, both treatments caused an increase in spermatogonia and spermatocytes, and a decrease in spermatozoa and locular area. In the Bidder's organ, the final diplotene oocytes increased, but the initial diplotene, degrading and atresic oocytes reduced in both treatments. The lipofuscin in the Bidder's organ was not affected. In the liver, melanin and lipofuscin increased only for the 1 mg/kg flutamide treatment. The 5 mg/kg treatment did not affect the liver. Serum testosterone and estradiol levels did not vary compared with the control group. This compound has antiandrogenic activity, which can affect the spermatogenetic process. The decrease in degrading and atresic Bidderian oocytes indicated that flutamide could stimulate the organ, retarding the degradation processes. The increase in liver melanin, which has protective role, and lipofuscin, a sign of degradation, indicates that flutamide cause hepatotoxic effects. So we conclude that flutamide negatively affects the testes, especially by reducing the sperm area, and the liver, inducing cell degradation and producing protective responses. Furthermore, the compound encourages lower degradation rates of the Bidder's organ germ cells.
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spelling Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideriBidder's organBufonidaeEndocrine disruptingEstradiolHepatic pigmentationTestisTestosteroneWater contamination is one of the factors influencing the decline of amphibians. Flutamide is an antiandrogenic medicine that occurs as water contaminant. This compound especially affects the reproductive organs, but it can also show hepatotoxic effects. The Bufonidae family has a peculiar organ named Bidder's organ, considered by some authors as a rudimentary ovary, but capable to respond to some external stimuli. This study investigated flutamide effects on testes and Bidder's organ germ cells, liver pigmentation, and sexual hormones levels in Rhinella schneideri males. We randomly divided 15 males in three groups (N = 5): two groups were injected with flutamide, at 1 and 5 mg/kg, while the control group received only mineral oil, for 7 days. After euthanasia, blood samples were collected and the organs were sent to histological routine. In the testes, both treatments caused an increase in spermatogonia and spermatocytes, and a decrease in spermatozoa and locular area. In the Bidder's organ, the final diplotene oocytes increased, but the initial diplotene, degrading and atresic oocytes reduced in both treatments. The lipofuscin in the Bidder's organ was not affected. In the liver, melanin and lipofuscin increased only for the 1 mg/kg flutamide treatment. The 5 mg/kg treatment did not affect the liver. Serum testosterone and estradiol levels did not vary compared with the control group. This compound has antiandrogenic activity, which can affect the spermatogenetic process. The decrease in degrading and atresic Bidderian oocytes indicated that flutamide could stimulate the organ, retarding the degradation processes. The increase in liver melanin, which has protective role, and lipofuscin, a sign of degradation, indicates that flutamide cause hepatotoxic effects. So we conclude that flutamide negatively affects the testes, especially by reducing the sperm area, and the liver, inducing cell degradation and producing protective responses. Furthermore, the compound encourages lower degradation rates of the Bidder's organ germ cells.Graduate Program in Animal Biology UNESP-Universidade Estadual Paulista Biology Department, Rua Cristóvão Colombo, 2265UNESP-Universidade Estadual Paulista Biology Department, Rua Cristóvão Colombo, 2265USP-Universidade de São Paulo Instituto de Biociências Physiology DepartmentGraduate Program in Animal Biology UNESP-Universidade Estadual Paulista Biology Department, Rua Cristóvão Colombo, 2265UNESP-Universidade Estadual Paulista Biology Department, Rua Cristóvão Colombo, 2265Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)de Gregorio, Lara S. [UNESP]Franco-Belussi, Lilian [UNESP]Gomes, Fernando R.de Oliveira, Classius [UNESP]2018-12-11T16:42:23Z2018-12-11T16:42:23Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article181-189application/pdfhttp://dx.doi.org/10.1016/j.aquatox.2016.04.022Aquatic Toxicology, v. 176, p. 181-189.1879-15140166-445Xhttp://hdl.handle.net/11449/16865210.1016/j.aquatox.2016.04.0222-s2.0-849667024372-s2.0-84966702437.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicology1,456info:eu-repo/semantics/openAccess2024-01-17T06:26:59Zoai:repositorio.unesp.br:11449/168652Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:16:19.156956Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
title Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
spellingShingle Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
de Gregorio, Lara S. [UNESP]
Bidder's organ
Bufonidae
Endocrine disrupting
Estradiol
Hepatic pigmentation
Testis
Testosterone
title_short Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
title_full Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
title_fullStr Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
title_full_unstemmed Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
title_sort Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri
author de Gregorio, Lara S. [UNESP]
author_facet de Gregorio, Lara S. [UNESP]
Franco-Belussi, Lilian [UNESP]
Gomes, Fernando R.
de Oliveira, Classius [UNESP]
author_role author
author2 Franco-Belussi, Lilian [UNESP]
Gomes, Fernando R.
de Oliveira, Classius [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv de Gregorio, Lara S. [UNESP]
Franco-Belussi, Lilian [UNESP]
Gomes, Fernando R.
de Oliveira, Classius [UNESP]
dc.subject.por.fl_str_mv Bidder's organ
Bufonidae
Endocrine disrupting
Estradiol
Hepatic pigmentation
Testis
Testosterone
topic Bidder's organ
Bufonidae
Endocrine disrupting
Estradiol
Hepatic pigmentation
Testis
Testosterone
description Water contamination is one of the factors influencing the decline of amphibians. Flutamide is an antiandrogenic medicine that occurs as water contaminant. This compound especially affects the reproductive organs, but it can also show hepatotoxic effects. The Bufonidae family has a peculiar organ named Bidder's organ, considered by some authors as a rudimentary ovary, but capable to respond to some external stimuli. This study investigated flutamide effects on testes and Bidder's organ germ cells, liver pigmentation, and sexual hormones levels in Rhinella schneideri males. We randomly divided 15 males in three groups (N = 5): two groups were injected with flutamide, at 1 and 5 mg/kg, while the control group received only mineral oil, for 7 days. After euthanasia, blood samples were collected and the organs were sent to histological routine. In the testes, both treatments caused an increase in spermatogonia and spermatocytes, and a decrease in spermatozoa and locular area. In the Bidder's organ, the final diplotene oocytes increased, but the initial diplotene, degrading and atresic oocytes reduced in both treatments. The lipofuscin in the Bidder's organ was not affected. In the liver, melanin and lipofuscin increased only for the 1 mg/kg flutamide treatment. The 5 mg/kg treatment did not affect the liver. Serum testosterone and estradiol levels did not vary compared with the control group. This compound has antiandrogenic activity, which can affect the spermatogenetic process. The decrease in degrading and atresic Bidderian oocytes indicated that flutamide could stimulate the organ, retarding the degradation processes. The increase in liver melanin, which has protective role, and lipofuscin, a sign of degradation, indicates that flutamide cause hepatotoxic effects. So we conclude that flutamide negatively affects the testes, especially by reducing the sperm area, and the liver, inducing cell degradation and producing protective responses. Furthermore, the compound encourages lower degradation rates of the Bidder's organ germ cells.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-01
2018-12-11T16:42:23Z
2018-12-11T16:42:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquatox.2016.04.022
Aquatic Toxicology, v. 176, p. 181-189.
1879-1514
0166-445X
http://hdl.handle.net/11449/168652
10.1016/j.aquatox.2016.04.022
2-s2.0-84966702437
2-s2.0-84966702437.pdf
url http://dx.doi.org/10.1016/j.aquatox.2016.04.022
http://hdl.handle.net/11449/168652
identifier_str_mv Aquatic Toxicology, v. 176, p. 181-189.
1879-1514
0166-445X
10.1016/j.aquatox.2016.04.022
2-s2.0-84966702437
2-s2.0-84966702437.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquatic Toxicology
1,456
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 181-189
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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