Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees?
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2017.06.113 http://hdl.handle.net/11449/174823 |
Resumo: | The use of insecticides on crops can affect non-target insects, such as bees. In addition to the adult bees, larvae can be exposed to the insecticide through contaminated floral resources. Therefore, this study aimed to investigate the possible effects of the exposure of A. mellifera larvae to a field concentration of thiamethoxam (0.001 ng/μL thiamethoxam) on larval and pupal survival and on the percentage of adult emergence. Additionally, its cytotoxic effects on the digestive cells of midgut, Malpighian tubules cells and Kenyon cells of the brain of newly emerged A. mellifera bees were analyzed. The results showed that larval exposure to this concentration of thiamethoxam did not influence larval and pupal survival or the percentage of adult bee emergence. However, this exposure caused ultra-structural alterations in the target and non-target organs of newly emerged bees. The digestive cell of bees that were exposed to the insecticide exhibited a basal labyrinth without long and thin channels and compromised mitochondria. In Malpighian tubules cells, disorganized basal labyrinth, dilated mitochondria with a deformed shape and a loss of cristae, and disorganized microvilli were observed. The results showed that the exposed bees presented Kenyon cells with alterations in the nucleus and mitochondria. These alterations indicate possible tissue degeneration, demonstrating the cytotoxicity of thiamethoxam in the target and non-target organs of newly emerged bees. Such results suggest cellular organelle impairment that can compromise cellular function of the midgut cells, Malpighian tubules cells and Kenyon cells, and, consequently, can compromise the longevity of the bees of the whole colony. |
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Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees?BeesMalpighian tubulesMidgutMushroom bodyNeonicotinoidUlstrastrucutureThe use of insecticides on crops can affect non-target insects, such as bees. In addition to the adult bees, larvae can be exposed to the insecticide through contaminated floral resources. Therefore, this study aimed to investigate the possible effects of the exposure of A. mellifera larvae to a field concentration of thiamethoxam (0.001 ng/μL thiamethoxam) on larval and pupal survival and on the percentage of adult emergence. Additionally, its cytotoxic effects on the digestive cells of midgut, Malpighian tubules cells and Kenyon cells of the brain of newly emerged A. mellifera bees were analyzed. The results showed that larval exposure to this concentration of thiamethoxam did not influence larval and pupal survival or the percentage of adult bee emergence. However, this exposure caused ultra-structural alterations in the target and non-target organs of newly emerged bees. The digestive cell of bees that were exposed to the insecticide exhibited a basal labyrinth without long and thin channels and compromised mitochondria. In Malpighian tubules cells, disorganized basal labyrinth, dilated mitochondria with a deformed shape and a loss of cristae, and disorganized microvilli were observed. The results showed that the exposed bees presented Kenyon cells with alterations in the nucleus and mitochondria. These alterations indicate possible tissue degeneration, demonstrating the cytotoxicity of thiamethoxam in the target and non-target organs of newly emerged bees. Such results suggest cellular organelle impairment that can compromise cellular function of the midgut cells, Malpighian tubules cells and Kenyon cells, and, consequently, can compromise the longevity of the bees of the whole colony.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP - Univ. Estadual Paulista campus de Rio Claro Instituto de Biociências Departamento de Biologia Centro de Estudos de Insetos SociaisUNESP - Univ. Estadual Paulista campus de Rio Claro Instituto de Biociências Departamento de Biologia Centro de Estudos de Insetos SociaisFAPESP: 2012/13370-8FAPESP: 2012/50197-2FAPESP: 2015/09691-1Universidade Estadual Paulista (Unesp)Friol, Priscila Sepúlveda [UNESP]Catae, Aline Fernanda [UNESP]Tavares, Daiana Antonia [UNESP]Malaspina, Osmar [UNESP]Roat, Thaisa Cristina [UNESP]2018-12-11T17:13:01Z2018-12-11T17:13:01Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article56-66application/pdfhttp://dx.doi.org/10.1016/j.chemosphere.2017.06.113Chemosphere, v. 185, p. 56-66.1879-12980045-6535http://hdl.handle.net/11449/17482310.1016/j.chemosphere.2017.06.1132-s2.0-850216330062-s2.0-85021633006.pdf75385560855058190000-0002-1650-257XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphere1,435info:eu-repo/semantics/openAccess2023-11-11T06:11:29Zoai:repositorio.unesp.br:11449/174823Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:22:39.979811Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
title |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
spellingShingle |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? Friol, Priscila Sepúlveda [UNESP] Bees Malpighian tubules Midgut Mushroom body Neonicotinoid Ulstrastrucuture |
title_short |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
title_full |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
title_fullStr |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
title_full_unstemmed |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
title_sort |
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees? |
author |
Friol, Priscila Sepúlveda [UNESP] |
author_facet |
Friol, Priscila Sepúlveda [UNESP] Catae, Aline Fernanda [UNESP] Tavares, Daiana Antonia [UNESP] Malaspina, Osmar [UNESP] Roat, Thaisa Cristina [UNESP] |
author_role |
author |
author2 |
Catae, Aline Fernanda [UNESP] Tavares, Daiana Antonia [UNESP] Malaspina, Osmar [UNESP] Roat, Thaisa Cristina [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Friol, Priscila Sepúlveda [UNESP] Catae, Aline Fernanda [UNESP] Tavares, Daiana Antonia [UNESP] Malaspina, Osmar [UNESP] Roat, Thaisa Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Bees Malpighian tubules Midgut Mushroom body Neonicotinoid Ulstrastrucuture |
topic |
Bees Malpighian tubules Midgut Mushroom body Neonicotinoid Ulstrastrucuture |
description |
The use of insecticides on crops can affect non-target insects, such as bees. In addition to the adult bees, larvae can be exposed to the insecticide through contaminated floral resources. Therefore, this study aimed to investigate the possible effects of the exposure of A. mellifera larvae to a field concentration of thiamethoxam (0.001 ng/μL thiamethoxam) on larval and pupal survival and on the percentage of adult emergence. Additionally, its cytotoxic effects on the digestive cells of midgut, Malpighian tubules cells and Kenyon cells of the brain of newly emerged A. mellifera bees were analyzed. The results showed that larval exposure to this concentration of thiamethoxam did not influence larval and pupal survival or the percentage of adult bee emergence. However, this exposure caused ultra-structural alterations in the target and non-target organs of newly emerged bees. The digestive cell of bees that were exposed to the insecticide exhibited a basal labyrinth without long and thin channels and compromised mitochondria. In Malpighian tubules cells, disorganized basal labyrinth, dilated mitochondria with a deformed shape and a loss of cristae, and disorganized microvilli were observed. The results showed that the exposed bees presented Kenyon cells with alterations in the nucleus and mitochondria. These alterations indicate possible tissue degeneration, demonstrating the cytotoxicity of thiamethoxam in the target and non-target organs of newly emerged bees. Such results suggest cellular organelle impairment that can compromise cellular function of the midgut cells, Malpighian tubules cells and Kenyon cells, and, consequently, can compromise the longevity of the bees of the whole colony. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:13:01Z 2018-12-11T17:13:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2017.06.113 Chemosphere, v. 185, p. 56-66. 1879-1298 0045-6535 http://hdl.handle.net/11449/174823 10.1016/j.chemosphere.2017.06.113 2-s2.0-85021633006 2-s2.0-85021633006.pdf 7538556085505819 0000-0002-1650-257X |
url |
http://dx.doi.org/10.1016/j.chemosphere.2017.06.113 http://hdl.handle.net/11449/174823 |
identifier_str_mv |
Chemosphere, v. 185, p. 56-66. 1879-1298 0045-6535 10.1016/j.chemosphere.2017.06.113 2-s2.0-85021633006 2-s2.0-85021633006.pdf 7538556085505819 0000-0002-1650-257X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere 1,435 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
56-66 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128801872281600 |