Genetic structure of Aedes aegypti populations determined using pairwise comparisons
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/2013.September.19.9 http://hdl.handle.net/11449/76599 |
Resumo: | The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated. Considering the six primers and the five populations, the mean value of inter-population genetic diversity (Gst) was 0.277, which is considered high according to the Wright classification. However, pairwise comparisons of the populations gave variable Gst values ranging from 0.044 to 0.289. This variation followed the population's geographic distance to some extent but was also influenced by human activity. The lowest Gst values were obtained in the comparison of populations from cities with intensive commercial and medical contacts. These mosquito populations were previously classified as insecticide resistant, susceptible, or with decreased susceptibility; this parameter apparently had an effect on the Gst values obtained in the pairwise comparisons. ©FUNPEC-RP. |
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Genetic structure of Aedes aegypti populations determined using pairwise comparisonsGenetic markersGeographic isolationInsecticide mortality percentagePassive migrationPolymorphic lociThe biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated. Considering the six primers and the five populations, the mean value of inter-population genetic diversity (Gst) was 0.277, which is considered high according to the Wright classification. However, pairwise comparisons of the populations gave variable Gst values ranging from 0.044 to 0.289. This variation followed the population's geographic distance to some extent but was also influenced by human activity. The lowest Gst values were obtained in the comparison of populations from cities with intensive commercial and medical contacts. These mosquito populations were previously classified as insecticide resistant, susceptible, or with decreased susceptibility; this parameter apparently had an effect on the Gst values obtained in the pairwise comparisons. ©FUNPEC-RP.Departamento de Biologia, Letras e Ciências Exatas Instituto de Biociências Universidade Estadual Paulista, São José do Rio Preto, SPSuperintendência de Controle de Endemias, Araçatuba, SPDepartamento de Biologia, Letras e Ciências Exatas Instituto de Biociências Universidade Estadual Paulista, São José do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Superintendência de Controle de Endemiasde Patarro, T. F. [UNESP]Guirado, M. M.Ravazzi, L. M. [UNESP]de Bicudo, H. E M C [UNESP]2014-05-27T11:30:42Z2014-05-27T11:30:42Z2013-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3775-3787application/pdfhttp://dx.doi.org/10.4238/2013.September.19.9Genetics and Molecular Research, v. 12, n. 3, p. 3775-3787, 2013.1676-5680http://hdl.handle.net/11449/7659910.4238/2013.September.19.9WOS:0003317174001692-s2.0-848845679202-s2.0-84884567920.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-10-17T06:07:48Zoai:repositorio.unesp.br:11449/76599Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:44.781661Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
title |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
spellingShingle |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons de Patarro, T. F. [UNESP] Genetic markers Geographic isolation Insecticide mortality percentage Passive migration Polymorphic loci |
title_short |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
title_full |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
title_fullStr |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
title_full_unstemmed |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
title_sort |
Genetic structure of Aedes aegypti populations determined using pairwise comparisons |
author |
de Patarro, T. F. [UNESP] |
author_facet |
de Patarro, T. F. [UNESP] Guirado, M. M. Ravazzi, L. M. [UNESP] de Bicudo, H. E M C [UNESP] |
author_role |
author |
author2 |
Guirado, M. M. Ravazzi, L. M. [UNESP] de Bicudo, H. E M C [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Superintendência de Controle de Endemias |
dc.contributor.author.fl_str_mv |
de Patarro, T. F. [UNESP] Guirado, M. M. Ravazzi, L. M. [UNESP] de Bicudo, H. E M C [UNESP] |
dc.subject.por.fl_str_mv |
Genetic markers Geographic isolation Insecticide mortality percentage Passive migration Polymorphic loci |
topic |
Genetic markers Geographic isolation Insecticide mortality percentage Passive migration Polymorphic loci |
description |
The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated. Considering the six primers and the five populations, the mean value of inter-population genetic diversity (Gst) was 0.277, which is considered high according to the Wright classification. However, pairwise comparisons of the populations gave variable Gst values ranging from 0.044 to 0.289. This variation followed the population's geographic distance to some extent but was also influenced by human activity. The lowest Gst values were obtained in the comparison of populations from cities with intensive commercial and medical contacts. These mosquito populations were previously classified as insecticide resistant, susceptible, or with decreased susceptibility; this parameter apparently had an effect on the Gst values obtained in the pairwise comparisons. ©FUNPEC-RP. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-19 2014-05-27T11:30:42Z 2014-05-27T11:30:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/2013.September.19.9 Genetics and Molecular Research, v. 12, n. 3, p. 3775-3787, 2013. 1676-5680 http://hdl.handle.net/11449/76599 10.4238/2013.September.19.9 WOS:000331717400169 2-s2.0-84884567920 2-s2.0-84884567920.pdf |
url |
http://dx.doi.org/10.4238/2013.September.19.9 http://hdl.handle.net/11449/76599 |
identifier_str_mv |
Genetics and Molecular Research, v. 12, n. 3, p. 3775-3787, 2013. 1676-5680 10.4238/2013.September.19.9 WOS:000331717400169 2-s2.0-84884567920 2-s2.0-84884567920.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3775-3787 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128475683356672 |