Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function

Detalhes bibliográficos
Autor(a) principal: Oliveira, Maria Emilia Franco [UNESP]
Data de Publicação: 2022
Outros Autores: Arrais, Aline Matos, Vergani, Gabriel Brun [UNESP], Novita Esteves, Sergio, Schinaider Do Amaral Pereira, Verônica, Garcia, Alexandre Rossetto, Bastos, Rosemary, Roberto Bourg Melo, Marco, Rios Coelho Alves, Bruna, Ferreira Fonseca, Jeferson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/10888705.2022.2141576
http://hdl.handle.net/11449/246268
Resumo: Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.
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spelling Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Functionanimal welfareEmbryo transfernon-surgicalpharmacologicaltranscervicalCervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)Embrapa Caprinos e Ovinos (CNPC)Universidade Federal Rural do Rio de Janeiro (UFRRJ)Embrapa Pecuária Sudeste (CPPSE)Universidade Estadual do Norte Fluminense Darcy RibeiroUniversidade Federal FluminenseFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal Rural do Rio de Janeiro (UFRRJ)Universidade Estadual do Norte Fluminense Darcy RibeiroUniversidade Federal Fluminense (UFF)Oliveira, Maria Emilia Franco [UNESP]Arrais, Aline MatosVergani, Gabriel Brun [UNESP]Novita Esteves, SergioSchinaider Do Amaral Pereira, VerônicaGarcia, Alexandre RossettoBastos, RosemaryRoberto Bourg Melo, MarcoRios Coelho Alves, BrunaFerreira Fonseca, Jeferson2023-07-29T12:36:12Z2023-07-29T12:36:12Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/10888705.2022.2141576Journal of Applied Animal Welfare Science.1532-76041088-8705http://hdl.handle.net/11449/24626810.1080/10888705.2022.21415762-s2.0-85141643567Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Animal Welfare Scienceinfo:eu-repo/semantics/openAccess2023-07-29T12:36:12Zoai:repositorio.unesp.br:11449/246268Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:30:33.151685Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
title Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
spellingShingle Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
Oliveira, Maria Emilia Franco [UNESP]
animal welfare
Embryo transfer
non-surgical
pharmacological
transcervical
title_short Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
title_full Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
title_fullStr Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
title_full_unstemmed Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
title_sort Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function
author Oliveira, Maria Emilia Franco [UNESP]
author_facet Oliveira, Maria Emilia Franco [UNESP]
Arrais, Aline Matos
Vergani, Gabriel Brun [UNESP]
Novita Esteves, Sergio
Schinaider Do Amaral Pereira, Verônica
Garcia, Alexandre Rossetto
Bastos, Rosemary
Roberto Bourg Melo, Marco
Rios Coelho Alves, Bruna
Ferreira Fonseca, Jeferson
author_role author
author2 Arrais, Aline Matos
Vergani, Gabriel Brun [UNESP]
Novita Esteves, Sergio
Schinaider Do Amaral Pereira, Verônica
Garcia, Alexandre Rossetto
Bastos, Rosemary
Roberto Bourg Melo, Marco
Rios Coelho Alves, Bruna
Ferreira Fonseca, Jeferson
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
Universidade Estadual do Norte Fluminense Darcy Ribeiro
Universidade Federal Fluminense (UFF)
dc.contributor.author.fl_str_mv Oliveira, Maria Emilia Franco [UNESP]
Arrais, Aline Matos
Vergani, Gabriel Brun [UNESP]
Novita Esteves, Sergio
Schinaider Do Amaral Pereira, Verônica
Garcia, Alexandre Rossetto
Bastos, Rosemary
Roberto Bourg Melo, Marco
Rios Coelho Alves, Bruna
Ferreira Fonseca, Jeferson
dc.subject.por.fl_str_mv animal welfare
Embryo transfer
non-surgical
pharmacological
transcervical
topic animal welfare
Embryo transfer
non-surgical
pharmacological
transcervical
description Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T12:36:12Z
2023-07-29T12:36:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/10888705.2022.2141576
Journal of Applied Animal Welfare Science.
1532-7604
1088-8705
http://hdl.handle.net/11449/246268
10.1080/10888705.2022.2141576
2-s2.0-85141643567
url http://dx.doi.org/10.1080/10888705.2022.2141576
http://hdl.handle.net/11449/246268
identifier_str_mv Journal of Applied Animal Welfare Science.
1532-7604
1088-8705
10.1080/10888705.2022.2141576
2-s2.0-85141643567
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Animal Welfare Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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